Whither or wither the Sulfur Anomaly in Planetary Nebulae?

We present a thorough investigation of the long standing sulfur anomaly enigma. Our analysis uses chemical abundances from the most extensive dataset available for 126 planetary nebulae (PNe) with improved accuracy and reduced uncertainties from a 10x10 degree Galactic bulge region. By using argon as a superior PNe metallicity indicator, the anomaly is significantly reduced and better constrained. For the first time in PNe we show sulfur alpha-element lock-step with both oxygen and argon. We dispel hypotheses that the anomaly originates from underestimation of higher sulfur ionization stages. Using a machine learning approach, we show that earlier ionization correction factor (ICFs) schemes contributed significantly to the anomaly. We find a correlation between the sulfur anomaly and the age/mass of PNe progenitors, with the anomaly either absent or significantly reduced in PNe with young progenitors. Despite inherent challenges and uncertainties, we link this to PNe dust chemistry, noting those with carbon-dust chemistry show a more pronounced anomaly. By integrating these findings, we provide a plausible explanation for the residual, reduced sulfur anomaly and propose its potential as an indicator of relative galaxy age compositions based on PNe.Comment: 12 pages, 4 figures main paper plus 3 figures in the appendi

Diverse Science from VLT imagery and spectroscopy of PNe in the Galactic Bulge

We have undertaken a deep investigation of a well defined sample of 136 PNe located in a 10x10 degree central region of the Galactic Bulge observed with the ESO VLT and supplemented by archival HST imagery. These studies have provided precise morphologies, major axes position angles and the most robust sample of consistently derived chemical abundances available to date. Using these data we have statistically confirmed, at 5-sigma, the precise PNe population that provides the PNe alignment of major axes previously suggested in the Galactic Bulge, revealed a partial solution to the sulfur anomaly and uncovered interesting morphological, abundance and kinematic features. We summarise the most significant findings here with detailed results appearing in a series of related publications.Comment: 6 pages, 5 figure

When the Stars Align: A 5 {\sigma} Concordance of Planetary Nebulae Major Axes in the Centre of our Galaxy

We report observations of a remarkable major axes alignment nearly parallel to the Galactic plane of 5{\sigma} significance for a subset of bulge "planetary nebulae" (PNe) that host, or are inferred to host, short period binaries. Nearly all are bipolar. It is solely this specific PNe population that accounts for the much weaker statistical alignments previously reported for the more general bulge PNe. It is clear evidence of a persistent, organised process acting on a measurable parameter at the heart of our Galaxy over perhaps cosmologically significant periods of time for this very particular PNe sample. Stable magnetic fields are currently the only plausible mechanism that could affect multiple binary star orbits as revealed by the observed major axes orientations of their eventual PNe. Examples are fed into the current bulge planetary nebulae population at a rate determined by their formation history and mass range of their binary stellar progenitors.Comment: 12 pages, 6 figures, 2 tables, matches version published in ApJ

Low-threshold optically pumped lasing in highly strained Ge nanowires

The integration of efficient, miniaturized group IV lasers into CMOS architecture holds the key to the realization of fully functional photonic-integrated circuits. Despite several years of progress, however, all group IV lasers reported to date exhibit impractically high thresholds owing to their unfavorable bandstructures. Highly strained germanium with its fundamentally altered bandstructure has emerged as a potential low-threshold gain medium, but there has yet to be any successful demonstration of lasing from this seemingly promising material system. Here, we demonstrate a low-threshold, compact group IV laser that employs germanium nanowire under a 1.6% uniaxial tensile strain as the gain medium. The amplified material gain in strained germanium can sufficiently surmount optical losses at 83 K, thus allowing the first observation of multimode lasing with an optical pumping threshold density of ~3.0 kW cm^-^2. Our demonstration opens up a new horizon of group IV lasers for photonic-integrated circuits.Comment: 31 pages, 9 figure

Robust estimation of bacterial cell count from optical density

Optical density (OD) is widely used to estimate the density of cells in liquid culture, but cannot be compared between instruments without a standardized calibration protocol and is challenging to relate to actual cell count. We address this with an interlaboratory study comparing three simple, low-cost, and highly accessible OD calibration protocols across 244 laboratories, applied to eight strains of constitutive GFP-expressing E. coli. Based on our results, we recommend calibrating OD to estimated cell count using serial dilution of silica microspheres, which produces highly precise calibration (95.5% of residuals <1.2-fold), is easily assessed for quality control, also assesses instrument effective linear range, and can be combined with fluorescence calibration to obtain units of Molecules of Equivalent Fluorescein (MEFL) per cell, allowing direct comparison and data fusion with flow cytometry measurements: in our study, fluorescence per cell measurements showed only a 1.07-fold mean difference between plate reader and flow cytometry data

Improvement in Salt Tolerance Ability of <i>Pseudomonas putida</i> KT2440

Pseudomonas putida KT2440 is a popular platform for bioremediation due to its robust tolerance to environmental stress and strong biodegradation capacity. Limited research on the salt tolerance of P. putida KT2440 has hindered its application. In this study, the strain KT2440 was tested to tolerate a maximum of 4% w/v NaCl cultured with minimal salts medium. Transcriptomic data in a high-salinity environment showed significant expression changes in genes in membrane components, redox processes, chemotaxis, and cellular catabolic processes. betB-encoding betaine-aldehyde dehydrogenase was identified from the transcriptome data to overexpress and enhance growth profile of the strain KT2440 in minimal salts medium containing 4% w/v NaCl. Meanwhile, screening for exogenous salt-tolerant genes revealed that the Na+/H+ antiporter EcnhaA from Escherichia coli significantly increased the growth of the strain KT2440 in 4% w/v NaCl. Then, co-expression of EcnhaA and betB (KT2440-EcnhaA-betB) increased the maximum salt tolerance of strain KT2440 to 5% w/v NaCl. Further addition of betaine and proline improved the salt tolerance of the engineered strain to 6% w/v NaCl. Finally, the engineered strain KT2440-EcnhaA-betB was able to degrade 56.70% of benzoic acid and 95.64% of protocatechuic acid in minimal salt medium containing 4% w/v NaCl in 48 h, while no biodegradation was observed in the normal strain KT2440 in the same conditions. However, the strain KT2440-EcnhaA-betB failed to degrade catechol in minimal salt medium containing 3% w/v NaCl. This study illustrated the improvement in the salt tolerance performance of Pseudomonas putida KT2440 and the feasibility of engineered strain KT2440 as a potential salt-tolerant bioremediation platform