Serum miR-423-5p levels are elavated in the patients with unstable angina pectoris (uAP) compared with stable angina pectoris (sAP) and aortic stenosis (AS).

<p>(A) Expression levels of miR-423-5p in the pericardial fluid (PF) and serum. (B) Expression levels of miR-423-5p in serum of patients with AS, sAP and uAP. miR-423-5p levels were using exogenous cel-miR-39. Data are presented as mean ± SD. *<i>P<0</i>.<i>05</i>. <i>**P<0</i>.<i>01</i>. (C) Expression levels of miR-423-5p in the PF of patients with AS, sAP and uAP. miR-423-5p levels were normalized using exogenous cel-miR-39. Data are presented as median and interquartile range. *<i>P<0</i>.<i>05</i>.</p

Muscle-enriched and vascular-enriched miRNAs are not elevated in PF compared with serum.

<p>(A) Expression levels of miR-133a in serum and PF, (B) Expression levels of miR-133a in the serum of patients with aortic stenosis (AS), stable angina pectoris (sAP) and unstable angina pectoris (uAP), (C) Expression levels of miR-92a in serum and PF, (D) Expression levels of miR-92a in serum of patients with AS, sAP and uAP, (E) Expression levels of miR-126 in serum and PF, (F) Expression levels of miR-126 in the serum of patients with AS, sAP and uAP. Each miRNA level was normalized using exogenous cel-miR-39. Data are presented as median and interquartile range. *<i>P<0</i>.<i>05</i>. <i>**P<0</i>.<i>01</i>. (G) Expression levels of miR-423-5p in cardiomyocytes and fibroblasts from neonatal mice hearts, n = 4. miR-423-5p was normalized using U6. Data are presented as mean ± SEM. <i>* p<0</i>.<i>05</i></p

Baseline Characteristics of Patients with sAP, uAP, and AS.

<p>sAP, stable angina pectoris; uAP, unstable angina pectoris; AS, aortic stenosis; LVDd, left ventricular end-diastolic dimension; LVDs, left ventricular end-systolic dimension; LVEF, left ventricular ejection fraction; ACEI, angiotensin-converting enzyme inhibitor; ARB, angiotensin receptor blocker; AST, aspartate aminotransferase; ALT, alanine aminotransferase; LDL-cho, low density lipoprotein; CK, creatine phosphokinase; eGFR, estimated glomerular filtration rate; Hb, hemoglobin; BNP brain natriuretic peptide; BS, blood sugar; HbA1c, and hemoglobin A1c. All values are expressed as mean ± SD.</p><p>Baseline Characteristics of Patients with sAP, uAP, and AS.</p

miR-423-5p and miR-3184-3p are complementary to each other.

<p>(A) Localization of nuclear speckle splicing regulatory protein 1 (NSRP1) on chromosome 17q11.2. MiR-423-5p is located in intron1 of NSRP1. MiR-423-5p and miR-3184-3p are encoded in opposite direction in the same region. (B) Sequence alignment between miR-423-5p and miR-3184-3p, miR-423-5p and miR-423-3p, and miR-3184-5p and miR-423-5p (C) miR-423-5p:miR-3184-3p and miR-423-5p:miR-133a were treated with 100μg/ml RNase A, and the expression level of miR-423-5p was measured at the indicated time points (n = 3). Data are presented as mean ± SEM. <i>*p<0</i>.<i>05</i>, <i>**p<0</i>.<i>01</i> (D) The correlation between the expression levels of miR-423-5p and miR-3184-3p in PF (n = 31). Each miRNAs was normalized using exogenous cel-miR-39.</p