Dialogic Public Relations Practice Through Corporate Websites Of Manufacturing Corporations In China

Corporate website has played an increasingly important role in China. Most Chinese corporate websites pay more attention to disseminating corporate information than building relationships with publics. Dialogic public relations is proved to be effective for relationship building on websites. This research aims to examine the dialogic public relations practice of Chinese manufacturing corporations through their websites. There are four research objectives: (1) to examine the characteristics of corporate websites in promoting communication and public relations; (2) to examine the characteristics of corporate websites in addressing various publics with tailored contents; (3) to examine the dialogic characteristics of corporate websites in facilitating dialogic relationship building; and (4) to provide strategies for corporate websites in promoting dialogic public relations

A New Descriptor for Amino Acids and Its Applications in Peptide QSAR

To establish a new amino acid structure descriptor that can be applied in peptide quantitative structure activity relationship (QSAR) Keywords: amino acids, peptides, quantitative structure-activity relationship (QSAR), SVMW descriptor Peptides are essential substance to sustain life In this paper, SVMW, which derived by principal components analysis of the matrix of 160 MoRSE descriptors and 99 WHIM descriptors of amino acids, were examined through principal component analysis (PCA). Applying SVMW to 58 angiotensin-converting enzyme inhibitors (dipeptide), 55 angiotensin-converting enzyme inhibitors (tri-peptides) and 48 bitter tasting thresholds, satisfying results were obtained from the constructed QSAR models. Experimental part Principle and Methodology Principal component analysis (PCA) Based on quantum chemistry calculation level of density function theory (DFT) Partial least square Partial least square (PLS) Stepwise multiple regression (SMR) was carried out for variable selection because it was less time-consuming and easy to implement. PLS was implemented by software of Simca-P 10.0. Matlab 7.0 was used for PCA, and SPSS 10.0 was used for stepwise multiple variable selection. Results and discussions QSAR model for angiotensin-converting enzyme inhibitors (dipeptide) Angiotensin converting enzyme inhibitor (dipeptide) is an inhibitor of angiotensin-converting enzyme (ACE)

Emerging Potential of Exosomes on Adipogenic Differentiation of Mesenchymal Stem Cells

The mesenchymal stem cells have multidirectional differentiation potential and can differentiate into adipocytes, osteoblasts, cartilage tissue, muscle cells and so on. The adipogenic differentiation of mesenchymal stem cells is of great significance for the construction of tissue-engineered fat and the treatment of soft tissue defects. Exosomes are nanoscale vesicles secreted by cells and widely exist in body fluids. They are mainly involved in cell communication processes and transferring cargo contents to recipient cells. In addition, exosomes can also promote tissue and organ regeneration. Recent studies have shown that various exosomes can influence the adipogenic differentiation of stem cells. In this review, the effects of exosomes on stem cell differentiation, especially on adipogenic differentiation, will be discussed, and the mechanisms and conclusions will be drawn. The main purpose of studying the role of these exosomes is to understand more comprehensively the influencing factors existing in the process of stem cell differentiation into adipocytes and provide a new idea in adipose tissue engineering research

Mechanical Automation Design and Manufacturing of Production Equipment Combined with Artificial Intelligence Technology and Algorithm Implementation

This paper explores the impact of artificial intelligence and machine automation on productivity. It focuses on analyzing the production model of machinery automation based on Petri nets. Through the invariant analysis method, the article constructs a model and presents its structure as a correlation matrix. The study results show that the model has an error rate of only 2.26% and an accuracy of 90.14% in terms of operational performance. Regarding time performance, its training time is 3854 seconds and response time is 351 milliseconds, which are better than other algorithms. In the practical application of automotive parts production, the method significantly reduces the busy probability and blocking probability during order processing and material delivery, and also improves the equipment load rate, waiting rate and blocking rate in the production process. This indicates that the Petri net-based method of machine automation production has significant advantages in improving productivity and reducing costs

Research on CNC programming and machining process based on CAD/CAM technology

Focusing on CNC process data, this paper provides an in-depth analysis, characterization and mining of macro machining processes by associating CAD and CAM models. The article introduces a Bayesian inference method to construct an association relationship between the manufacturing features of the query part and the macroscopic process of the manufactured part, which avoids the need for a direct similarity comparison between the geometry of the query part and the part to be manufactured. In addition, the study calculated the overall similarity between the query part and the CNC programming process data instances through the guidance of the process skeleton, thus realizing the precise evaluation from the CNC programming perspective. The algorithm for generating tool trajectories in multi-axis CNC programming is analyzed. By selecting representative surfaces and performing tool processing calculations, this study explores the process planning and tool parameter selection of usual parts of surfaces in multi-axis CNC programming machining. It optimizes the nonlinear error in tool trajectory processing by combining CAD/CAM technology. The results show that the optimized process proposed in this paper significantly improves the machining efficiency by 36.94%. In the cutting at tool corners, the maximum cutting force generated by the optimized machining process is only 190.3N, which is only 67.2% of the leading cutting force of the Cimatron process. In addition, when the engagement frequency is 780 Hz, the optimized process proposed in this paper has the smallest vibration amplitude, which is only 21.4% of the Cimatron strategy. Therefore, this study significantly improves the machining efficiency while ensuring the machining quality, which has important practical application value

p62 is linked to mitophagy in oleic acid-induced adipogenesis in human adipose-derived stromal cells

Abstract Background Obesity is closely related to the abnormal differentiation of adipocytes, which are subjected to high plasma levels of free fatty acids (FFAs). As the most abundant FFA in the bloodstream, oleic acid (OA) has the ability to induce adipogenic differentiation in human adipose-derived stromal cells (hADSCs). Recently, p62, an autophagy mediator, has been shown to play a role in obesity and adipose tissue metabolism. Therefore, the aim of this study was to investigate the roles of autophagy and mitochondrial function at different stages of OA (in combination with insulin and dexamethasone)-induced adipogenesis in hADSCs. Methods The hADSCs were incubated with OA, insulin, and dexamethasone after pretreatment with autophagy inhibitors or knockdown of p62 with shRNA. The adiposeness level was then analyzed by oil red O staining in the cells. The related proteins or mRNA levels were detected by western blot analysis or quantitative real-time polymerase chain reaction (PCR). Results Treatment with 80 μM OA (substituted for isobutylmethylxantine; IBMX) for 10 days successfully induced hADSCs to adipocytes. During OA-induced adipogenesis, autophagy was induced, with an increased LC3II/I ratio on day 3 and a decreased protein level of p62 on and after day 3. Inhibition of autophagy with 3-methyladenine (3MA) at the early stage (day 0 to day 3) of differentiation, but not at the middle or late stage, significantly decreased OA-induced adipogenesis; while knockdown of p62 with shRNA significantly promoted adipogenesis in hADSCs. Moreover, the copy number of mtDNA (the ND1 gene) and the protein level of TOM20, a mitochondrial membrane protein, were increased following OA treatment, which was related to the stability of mitochondria. Interestingly, knockdown of p62 increased the mito-LC3II/I and cyto-LC3II/I ratios by 110.1% and 73.3%, respectively. The increase in the ratio of mito-LC3II/I was higher than that of cyto-LC3II/I. Furthermore, p62 knockdown-enhanced adipogenesis in hADSCs was abolished by inhibiting mitophagy with cyclosporine A. Conclusions These results suggested that p62 plays a protective role in adipogenesis of hADSCs through regulating mitophagy

Residual Characteristics and Risk Assessment of Hexabromocyclododecane in Humans from 2010 to 2018 in Beijing

BACKGROUND Hexabromocyclododecanes (HBCDs) have been identified as organic pollutants with the properties of persistence, long-distance migration, bioaccumulation, and biological toxicity. HBCDs have been detected in all kinds of biological and abiotic samples around the world, and have adverse effects on the environment and human body. As a non-invasive biological sample, human milk is a good medium for evaluating the exposure of HBCDs in the human body, and is widely used in the study of the exposure level of HBCDs in various countries, especially in nursing infants and young children. According to the survey, the production and use of HBCDs in Beijing has continued to rise since the use and production restriction of HBCDs in 2016, and the total use of HBCDs in 2019 increased by 60.8% compared with 2018. Therefore, in recent years in Beijing, especially before and after the use and production restriction of HBCDs, the residual characteristics and change trend of HBCDs in breast milk have attracted much attention. OBJECTIVES To investigate the residual characteristics and trends of HBCDs in breast milk in Beijing from 2010 to 2018, evaluate the health risks for infants to intake HBCDs via breastfeeding, and provide basic data support for the scientific use, management, control, and human exposure risk assessment of HBCDs. METHODS Breast milk samples (n=233) were obtained from 85 volunteer donors living in Beijing from 2010 to 2018. All volunteers have been living in Beijing for more than 5 years, are healthy, and do not directly engage in HBCDs production, processing, and other related occupations. Each sample was about 30mL, which was manually collected by volunteers into a 30mL clean glass bottle with a Teflon lined screw cap, and then transferred to a laboratory refrigerator for cryopreservation at -20℃. All volunteers were informed of the objective of this study. Pretreatment was conducted by liquid-liquid extraction and ammonia, ethanol, ether and n-hexane were added in turn. After liquid-liquid extraction, concentrated sulfuric acid sulfonation was combined with solid phase extraction (SPE) to purify the samples. Finally, 200μL methanol was added for constant volume. After centrifugation, the upper layer solution was transferred to a brown sample bottle (including lined tube) for storage. The qualitative and quantitative analysis of HBCDs was performed by Waters UPLC CLASS ultra-high performance liquid phase tandem API 4000 triple quadrupole mass spectrometry(LC-MS/MS). The isotope internal standard method was used for quantitative analysis to reduce the matrix effect. RESULTS The detection rate of HBCDs in breast milk was 100%, indicating that HBCDs have been widespread in the human body. The concentration range of HBCDs was 0.46-93.5ng/g lw (lipid weight), with the average and median values of 7.27ng/g lw and 5.77ng/g lw, respectively. The 95th percentile of high-end exposure was 15.6ng/g lw. There were individual differences in the milk samples. The detection rates of α, β and γ-HBCD in all breast milk samples were 100%, 35.6% and 70.8%, respectively. α-HBCD had the most abundant diastereomers in breast milk samples, accounting for 80.1%, followed by γ-HBCD and β-HBCD in order. There were also a small number of breast milk samples that were dominated by γ-HBCD, accounting for 52.2%-83.5%. Even samples taken by the same volunteer in different months had different major diastereomers. The median EDI of HBCDs via human milk for infant was 20.5ng/kg bw/day, and the 95th percentile value was 71.4ng/kg bw/day, which was comparable to the previous research in Beijing, but higher than Japan, Ghana and other cities in China. CONCLUSIONS Compared with the HBCDs in breast milk from other regions at home and abroad, the level of HBCDs in breast milk in this study is close to the previous research data in Beijing, but higher than that of Shanghai, Shenzhen and other cities in China, as well as higher than the results of recent studies in Japan, the United Kingdom, the United States, Canada, India, the Philippines and some African countries, which may be related to the continuous use of HBCDs in China and the large amount of use. In human breast milk, α-HBCD is the main diastereomers, while in a small number of samples, γ-HBCD is the main diastereomers. The differences in the distribution characteristics of these diastereomers may be due to the differences in the absorption, metabolism and excretion mechanisms among individuals, and the transformation of HBCDs among different isomers in vivo, and the differences in the main external exposure pathways. The level of HBCDs showed an obvious upward trend from 2010 to 2013, reached the highest value of 6.84ng/g lw in 2013, and then decreased slightly and remained constant, which may be related to the production, use and prohibition of HBCDs globally and domestically. The time trend of α-HBCD and HBCDs is basically the same, while the content of β-HBCD and γ-HBCD in breast milk is low, and the change trend is stable with time. The concentration of HBCDs in breast milk changed slowly from 2014 to 2018 but did not show a significant downward trend after the initial ban of HBCDs in China in 2016, which may indicate that there are still a large number of HBCDs in production and use on the market, and human intake of HBCDs through air, dust, diet and other ways does not decrease. The risk assessment results indicate that the Hazard Quotient (HQ) of 99% breast milk samples is less than 1, which indicates that the intake of HBCDs through breast-feeding during lactation would not bring significant health risk to infants, but the HQ value of 1% breast milk samples is greater than 1, which suggests that breastfeeding may bring health risks to infants. More research is needed to further investigate the major sources of exposure and the effect of each potential factor

Obstructive jaundice due to a blood clot after ERCP: a case report and review of the literature

Abstract Background Endoscopic retrograde cholangiopancreatography (ERCP) is one of the most frequently performed procedures for the treatment of biliary-pancreatic diseases. The most frequent complications of ERCP include pancreatitis, haemorrhage, perforation and cholangitis. While post-ERCP biliary bleeding leading to biliary obstruction is rare. Case presentation We herein report a case of exceptional post-ERCP cholangitis due to a blood clot in the common bile duct (CBD). This case involves a 75-year-old woman with a history of recurring upper abdominal pain. Abdominal computerized tomography (CT) revealed dilatation of the extrahepatic bile duct with stones at the lower CBD. After ERCP, clearance of stones was obtained. The post-ERCP course was symptomatic with upper abdominal pain and a significant increase in cholestatic parameters. A second CT scan demonstrated a markedly dilated biliary tree with a longitudinal high-density image at the middle CBD. The patient was successfully treated with a repeated ERCP, and a blood clot was extracted. We also present a review of the literature published between 1985 and 2016 in PubMed. Four similar cases were reported during this period from France, Turkey, the USA and the UK, separately. Our case is the first reported in China. Conclusions Post-ERCP biliary bleeding leading to biliary obstruction is rare. We describe a rare case of post-ERCP cholangitis due to a blood clot in the common bile duct (CBD), which is consistent with most clinical presentations of similar cases already described. An analysis of the possible pathophysiological mechanisms and a review of the current literature are provided. We attempt to attract clinicians’ attention to the differential diagnosis of post-ERCP obstruction. The complications might be severe or even fatal. The diagnosis of blood clot is based on clinical and laboratory data, particularly imaging. Repeated ERCP is often necessary and effective