Adaptive changes in the ornithine cycle and amino acid synthesis in sheep liver with different meat productivity

The aim of the research was to study the ornithine cycle as the process of fixing ammonia and the formation of urea in the body of highly productive animals. In our experiments, we used a protein-deficient diet and urea as a nitrogen substitute for nitrogen-containing materials in the diet to reveal the mechanism of action of urea on animals, in particular on the biochemical processes of the ornithine cycle. There are some differences between Bukovinian sheep of the Askanian meat-wool breed and outbreds in terms of the ability to build muscle tissue. Our study reveals that the slaughter yield and the average daily gain consumption of Bukovinian-type meat of the Askanian meat-wool breed were higher in summer and in autumn, compare with purebred sheep. Sheep of the Bukovynian type of Askanian meat-wool breed have the intensity of enzymatic formation of urea in liver homogenates that is much higher in all experiments than in outbred sheep. A sharp drop in the activity of all stages of urea formation and glutamic acid synthesis in liver homogenates and significantly weakened urea formation was found in all experiments of the fourth series in comparison with the experiments in the third series. Increased muscle growth, high nitrogen deposition, and a much lower percentage of urinary excretion of ammonia and urea nitrogen, as well as higher activity of enzymes of the ornithine cycle and glutamic acid synthesis in the Bukovinian sheep type of Askanian meat-wool breed compared to outbreeds allow concluding that ammonia and urea in highly productive animals act less as finishing products of nitrogen metabolism than in low-productive animals

Biochemical parameters of chicken blood under the influence of technological stimuli of various etiologies

Violation of the main technological parameters of keeping and feeding poultry is the main factor in reducing egg productivity and health in the industrial production system. Therefore, the research aims to determine biochemical markers in acute technological stress caused by factors of various etiologies. In this study, serum biochemical parameters were evaluated for the effects of stimuli in egg-laying chickens. The study was conducted on several Hy-Line W-36 cross chickens in the conditions of a production line. Four groups of chickens (101 heads each) aged 52 weeks were formed to conduct the study. The control group was formed from 10 representatives of each of the groups. Further, each group was exposed to a corresponding technological stimulus. Birds of group 2 were not fed for 24 hours, those of group 3 were kept in a darkened place, and 60 additional birds were placed in a cage with the chickens of group 4. The highest effect on the dynamics of biochemical parameters was an increase in their concentration by 1 m2 cage space. The studies identified a substantial increase in the concentration of glucose and creatinine in the blood serum of chickens during over-compaction – by 24-28% (p<0.05) compared to the control group, regardless of the cause of the stressful situation, all birds exposed to acute stress had an increase in these indicators in the blood serum. The main part of the examined enzymes, such as aspartate aminotransferase, alkaline phosphatase, and lactate dehydrogenase, tended to increase their activity depending on the strength of the influence of the technological factor on the body of birds. Dynamic characteristics of glucose and creatinine levels were distinguished among the examined biochemical parameters by rapid and differentiated reactions within the physiological norm, and considering the simplicity of their determination, they may have prospects of becoming the main markers of early diagnosis of stress in industrial poultry farmin

Modeling of Microbiological and Biochemical Processes under the Conditions of Steam Contact Sterilization in Containers of Turkey Meat Pate

The article proposes modes of sterilization of meat pate from turkey meat, which was determined by the method in which the actual lethality Ff relative to the microflora should be equal to or exceed the required lethality of the sterilization process Fn (Ff ≥ Fn) canned turkey pate. The results of the study of the dependence of the kinetics of biochemical reactions on the thermal, chemical and mechanical sensitivity of the processed product, on pressure, temperature  and chemical potential are presented. One of the effective ways to intensify the heat transfer process is the contact heating of the product by means of steam supply, which has a technological effect on the processed products. When comparing the results of the simulated results with experimental data, it is established that the solution of the problem of calculating the temperature field of the product on a computer gives quite satisfactory results between the calculated and experimental data. It is established that as a modification for the processed products it is necessary to solve thermohydromechanical equations with the corresponding initial and boundary conditions in addition to the transfer equation for each scalar quantity