STIM/Orai-mediated calcium entry elicits spontaneous TSLP overproduction in epidermal cells of atopic dermatitis mice

Aim: Atopic dermatitis (AD) is a pruritic, chronic inflammatory skin disease. Thymic stromal lymphopoietin (TSLP) is highly expressed in the epidermis of patients with AD and induces T helper 2 (Th2) immune responses and itching. Although the mechanism underlying the stimulus-induced TSLP production in normal keratinocytes has been intensively studied, whether the production capability of TSLP is naturally enhanced in epidermal cells in AD conditions remains unclear. Previous studies demonstrated that a deficiency of polyunsaturated fatty acid (PUFA) causes AD-like pruritic skin inflammation in special diet-fed hairless mice. The aim of the study was to examine the TSLP production capability of epidermal cells isolated from diet-induced AD mouse model and its mechanism. Methods: Epidermal cells were isolated from normal and AD mice and incubated under unstimulated culture conditions to assess spontaneous TSLP production. Messenger ribonucleic acid (mRNA) and protein levels of TSLP were determined by real-time polymerase chain reaction (PCR) and enzyme-linked immunosorbent assay (ELISA), respectively. Results: TSLP level was markedly increased in the skin of AD mice. When epidermal cells were isolated from AD mice and cultured without stimulation, Tslp gene expression was upregulated, and a large amount of TSLP protein was extracellularly released. Such TSLP overproduction was not observed in the epidermal cells of normal mice. TSLP overproduction in AD epidermal cells was almost completely inhibited by extracellular calcium chelation, interference with plasma membrane interaction of stromal interaction molecule 1 (STIM1), blockade of the calcium release-activated calcium (CRAC) channels Orai1 and Orai2, or treatment with a PUFA γ-linolenic acid (GLA). Conclusions: Epidermal cells isolated from AD mice can spontaneously produce TSLP through STIM/Orai-mediated calcium entry, and GLA may negatively regulate this TSLP production

Behavior of the Blade Tip Vortices of a Wind Turbine Equipped with a Brimmed-Diffuser Shroud

To clarify the behavior of the blade tip vortices of a wind turbine equipped with a brimmed-diffuser shroud, called a “Wind-Lens turbine”, we conducted a three-dimensional numerical simulation using a large eddy simulation (LES). Since this unique wind turbine consists of not only rotating blades but also a diffuser shroud with a broad-ring brim at the exit periphery, the flow field around the turbine is highly complex and unsteady. Previously, our research group conducted numerical simulations using an actuator-disc approximation, in which the rotating blades were simply modeled as an external force on the fluid. Therefore, the detailed flow patterns around the rotating blades and the shroud, including the blade tip vortices, could not be simulated. Instead of an actuator-disc approximation, we used a moving boundary technique in the present CFD simulation to simulate the flow around a rotating blade in order to focus especially on blade tip vortices. The simulation results showed a pair of vortices consisting of a blade tip vortex and a counter-rotating vortex which was generated between the blade tip and the inner surface of the diffuser. Since these vortices interacted with each other, the blade tip vortex was weakened by the counter-rotating vortex. The results showed good agreement with past wind tunnel experiments

The effect of a running task on muscle shear elastic modulus of posterior lower leg

Abstract Background Medial tibial stress syndrome (MTSS) is one of the most common causes of exercise-related leg pain in runners. Because stopping training due to pain from MTSS could decrease the athlete’s competitiveness, it is necessary to construct MTSS prevention and treatment programs. However, the effect of running, which is believed to cause MTSS, on shear elastic modulus of the posterior lower leg is unclear. Therefore, the purpose of this study was to investigate the effect of 30 min of running on shear elastic modulus of the posterior lower leg in healthy subjects. Methods Twenty healthy males volunteered to participate in this study (age, 20.9 ± 0.6 y; height, 169.6 ± 4.5 cm; weight, 62.6 ± 5.2 kg). The shear elastic modulus of the posterior lower leg was measured using ultrasonic shear wave elastography before and immediately after a 30-min running task. Results Shear elastic moduli of the flexor digitorum longus and tibialis posterior were significantly increased after 30 min running task. However, there were no significant changes in shear elastic moduli of the lateral gastrocnemius, medial gastrocnemius, peroneus longus and peroneus brevis. Conclusion The results suggested that the increases in shear elastic moduli of flexor digitorum longus and tibialis posterior after running could be a risk factor for running-related MTSS development

Two cases of adult onset Schonlein-Henoch purpura with gastrointestinal manifestations

We describe two cases of adult-onset Schonlein-Henoch purpura with gastrointestinal manifestations. Case 1: A 73-year-old man was admitted to our hospital because of abdominal pain, diarrhea and fever. On physical examination, the abdomen was normal, and there were many palpable petechial hemorrhages on the lower extremities.Skin biopsy revealed infiltrations of lymphocytes, neutrophils and eosinophils into the dermis. A computed tomographic (CT) scan of the abdomen disclosed thickening of the intestinal wall in the duodenum and jejunum.Upper gastrointestinal fiberscopy revealed petechiation on the stomach and erosions on the duodenum. Case 2: A 54-year-old man was admitted to our hospital because of hematochezia.The patient had pharyngitis 4 days before admission, and his serum anti-streptolysion O level was increased.On physical examination, there were faint purpuras on the lower extremities.Skin biopsy revealed a histological image of leukocytoclastic vasculitis. A CT scan of the abdomen disclosed thickening of the intestinal wall in the duodenum, jejunum and ileum.Gastrointestinal fiberscopy revealed petechiation on the stomach and ulceration on the cecum

Daily Cisplatin and Weekly Docetaxel versus Weekly Cisplatin Intra-Arterial Chemoradiotherapy for Late T2-3 Tongue Cancer: A Pilot and Feasibility Trial

Background and objectives: The aim of present study was to compare the treatment results of daily cisplatin (CDDP), weekly docetaxel (DOC) intra-arterial infusion chemotherapy combined with radiotherapy (DIACRT) regimen and weekly CDDP intra-arterial infusion chemotherapy combined with radiotherapy (WIACRT) for patients with tongue cancer. Materials and Methods: Between January 2007 and December 2016, a total of 11 patients treated with WIACRT and 45 patients treated with DIACRT were enrolled in the present study. In the DIACRT group, 25 patients had late T2, and 20 patients had T3. A total of nine patients had late T2 and two had T3 in WIACRT (p = NS). In DIACRT, the treatment schedule consisted of intra-arterial chemotherapy (DOC, total 60 mg/m2; CDDP, total 150 mg/m2) and daily concurrent radiotherapy (RT) (total, 60 Gy). In WIACRT, the treatment schedule consisted of intra-arterial chemotherapy (CDDP, total 360 mg/m2) and daily concurrent RT (total, 60 Gy). Results: The median follow-up periods for DIACRT and WIACRT were 61 and 66 months, respectively. The five-year local control (LC) and overall survival (OS) rate were 94.5% and 89.6% for the DIACRT group, and 60.6% and 63.6% for the WIACRT group, respectively. The LC rate and OS of the DIACRT group were significantly higher than those of the WIACRT group. As regards toxicities, no treatment-related deaths were observed during the follow-up periods in both groups. Conclusions: DIACRT was found to be feasible and effective for patients with tongue cancer and could become a new treatment modality