Analysis and comparison of amino acid mutations in ORF5 gene of 32 Chinese isolates and 9 reference strains.

<p>Dots (.) indicate the same amino acids as in VR2332 and substitutions are indicated by the amino acid letter codes. The functional domains indicated by color boxes are reported according to Zhou et al. <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0033756#pone.0033756-Zhou1" target="_blank">[11]</a>.</p

Phylogenetic relationship of PRRSVs.

<p>A neighbor-joining tree was constructed based on sequences of the ORF5 gene of PRRSV, with bootstrap values calculated from 1,000 replicates. Subgroups are marked with different colors and the strains isolated in this study are indicated in bold.</p

Representative PRRSV Strains used in the phylogenetic and sequence analyses.

*<p>JX-A1 is the representative strain of HP-PRRSV in China since 2006;</p>#<p>Ch-1a is the first PRRSV isolate in mainland China in 1996.</p

Analysis of nucleotide and amino acid identity of ORF5 among 32 PRRSV isolates and with represent PRRSV isolates LV, VR-2332, RespPRRSV, Ch-1a, and highly pathogenic strain JX-A1.

<p>Analysis of nucleotide and amino acid identity of ORF5 among 32 PRRSV isolates and with represent PRRSV isolates LV, VR-2332, RespPRRSV, Ch-1a, and highly pathogenic strain JX-A1.</p