3 research outputs found
Lipid-Based Liquid Crystalline Nanoparticles Facilitate Cytosolic Delivery of siRNA via Structural Transformation
RNA
interference (RNAi) technology has shown great promise for
the treatment of cancer and other genetic disorders. Despite the efforts
to increase the target tissue distribution, the safe and effective
delivery of siRNA to the diseased cells with sufficient cytosolic
transport is another critical factor for successful RNAi clinical
application. Here, the constructed lipid-based liquid crystalline
nanoparticles, called nano-Transformers, can transform thestructure
in the intracellular acidic environment and perform high-efficient
siRNA delivery for cancer treatment. The developed nano-Transformers
have satisfactory siRNA loading efficiency and low cytotoxicity. Different
from the traditional cationic nanocarriers, the endosomal membrane
fusion induced by the conformational transition of lipids contributes
to the easy dissociation of siRNA from nanocarriers and direct release
of free siRNA into cytoplasm. We show that transfection with cyclin-dependent
kinase 1 (CDK1)-siRNA-loaded nano-Transformers causes up to 95% reduction
of relevant mRNA <i>in vitro</i> and greatly inhibits the
tumor growth without causing any immunogenic response <i>in vivo</i>. This work highlights that the lipid-based nano-Transformers may
become the next generation of siRNA delivery system with higher efficacy
and improved safety profiles
Lipid-Based Liquid Crystalline Nanoparticles Facilitate Cytosolic Delivery of siRNA via Structural Transformation
RNA
interference (RNAi) technology has shown great promise for
the treatment of cancer and other genetic disorders. Despite the efforts
to increase the target tissue distribution, the safe and effective
delivery of siRNA to the diseased cells with sufficient cytosolic
transport is another critical factor for successful RNAi clinical
application. Here, the constructed lipid-based liquid crystalline
nanoparticles, called nano-Transformers, can transform thestructure
in the intracellular acidic environment and perform high-efficient
siRNA delivery for cancer treatment. The developed nano-Transformers
have satisfactory siRNA loading efficiency and low cytotoxicity. Different
from the traditional cationic nanocarriers, the endosomal membrane
fusion induced by the conformational transition of lipids contributes
to the easy dissociation of siRNA from nanocarriers and direct release
of free siRNA into cytoplasm. We show that transfection with cyclin-dependent
kinase 1 (CDK1)-siRNA-loaded nano-Transformers causes up to 95% reduction
of relevant mRNA <i>in vitro</i> and greatly inhibits the
tumor growth without causing any immunogenic response <i>in vivo</i>. This work highlights that the lipid-based nano-Transformers may
become the next generation of siRNA delivery system with higher efficacy
and improved safety profiles
Lipid-Based Liquid Crystalline Nanoparticles Facilitate Cytosolic Delivery of siRNA via Structural Transformation
RNA
interference (RNAi) technology has shown great promise for
the treatment of cancer and other genetic disorders. Despite the efforts
to increase the target tissue distribution, the safe and effective
delivery of siRNA to the diseased cells with sufficient cytosolic
transport is another critical factor for successful RNAi clinical
application. Here, the constructed lipid-based liquid crystalline
nanoparticles, called nano-Transformers, can transform thestructure
in the intracellular acidic environment and perform high-efficient
siRNA delivery for cancer treatment. The developed nano-Transformers
have satisfactory siRNA loading efficiency and low cytotoxicity. Different
from the traditional cationic nanocarriers, the endosomal membrane
fusion induced by the conformational transition of lipids contributes
to the easy dissociation of siRNA from nanocarriers and direct release
of free siRNA into cytoplasm. We show that transfection with cyclin-dependent
kinase 1 (CDK1)-siRNA-loaded nano-Transformers causes up to 95% reduction
of relevant mRNA <i>in vitro</i> and greatly inhibits the
tumor growth without causing any immunogenic response <i>in vivo</i>. This work highlights that the lipid-based nano-Transformers may
become the next generation of siRNA delivery system with higher efficacy
and improved safety profiles