Additional file 1 of Indigofera suffruticosa aerial parts extract induce G2/M arrest and ATR/CHK1 pathway in Jurkat cells

Additional file 1: Supplementary Table S1. Antibodies used in this study. Supplementary Fig. S1. Representative plot of Annexin V staining results. This figure is related to Fig. 2D. Supplementary Fig. S2. The caspase-3/7 activities in Jurkat cells after 12 h of ISAE treatment were assessed by Caspase-Glo® 3/7 assay (Promega) according to the manufacturer's instructions. The graph was expressed as fold changes to control group. Supplementary Fig. S3. Relative quantitative analysis of tryptanthrin, indigo, and indirubin in ISAE. The selected ion current chromatograms of the ISAE extract, tryptanthrin, indigo, and indirubin were carried out through MS full scan experiment in positive mode. The relative abundance of the three selected compounds in ISAE extract were calculated based on comparing the peak area ratios with standard compounds. Supplementary Fig. S4. Cell gating for cell cycle analysis. For cell cycle analysis, cells were gated using forward scatter (FSC) and side scatter (SSC) properties. This helped in excluding cell debris and selecting the desired cells (within the black circle) for analysis. The gating area was established based on the solvent control group (0 μg/mL of ISAE) and was consistently applied to all other groups. This illustration corresponds to Fig. 2A. Original images of western blot. Multiple exposure images of WB