La Charente

21 septembre 18861886/09/21 (A15,N5666)-1886/09/21.Appartient à l’ensemble documentaire : PoitouCh

Additional file 2: Figure S2. of Novel functions of LHX2 and PAX6 in the developing telencephalon revealed upon combined loss of both genes

Tamoxifen was administered at E8.5 to CreER;Lhx2 lox/lox ;Pax6 lox/lox animals and the embryos were harvested at E12.5. Near-complete recombination of Pax6 and Lhx2 is seen in a rostro-caudal series of sections adjacent to those examined for Wnt8b expression. (JPEG 807 kb

Additional file 3: Figure S3. of Novel functions of LHX2 and PAX6 in the developing telencephalon revealed upon combined loss of both genes

This figure shows Dbx1 expression in adjacent sections to the images shown in Fig. 3. These images were used for measurements of the hem and the dorsal telencephalic neuroepithelium, taken upto the ventral extent of Dbx1 in adjacent sections in CreERT2; Lhx2 lox/lox (dashed lines, B,D,F) and CreER;Lhx2 lox/lox ;Pax6 lox/lox (H,J,L) embryos. Dbx1 expression in the control brain is not shown. Scale bar is 200 Îźm. (JPEG 856 kb

S2 Fig -

(A, B) KEGG pathway analysis of Ncp and Hcp enriched genes related to Fig 1B. (C-D) Motif analysis shows known motifs from 70 DARs (Ncp) and 14804 DARs (Hcp) related to Fig 2B. (E) Expression of many of the transcription factors identified among the top 10 motifs is undetectable in the E11.5 Ncp or Hcp (as obtained from; Allen Mouse Brain Atlas, http://mouse.brain-map.org/). Links to images represented in E: Dlx1Dlx2Dlx5Lhx1Lhx3Isl1Nkx6.1En1Rfx2Rfx5Xbp1. (TIF)</p

S4 Fig -

(A-F) GO: BPs corresponding to both up-and down-regulated genes upon loss of Lhx2 in the Ncp (A-C) and Hcp (D-F). (A, D) show the GSEA analysis and (B, C, E, F) show the overrepresentation test analysis. (TIF)</p

S5 Fig -

(A, B) KEGG pathway analysis of direct targets of LHX2 from Fig 5 (F) reveal 4 dysregulated pathways common to the E12.5 Ncp and Hcp. (C-F) KEGG pathway analysis for these pathways includes both direct (*) and indirect targets of LHX2. (E, F). (TIF)</p

Transcriptomic analysis of the wild type E12.5 Ncp and Hcp.

(A) Schematic representation of the E12.5 mouse brain. (B) A volcano plot comparing Ncp and Hcp mRNA expression identifies preferentially enriched genes in the Ncp (1248) and the Hcp (1364). (C) A heatmap of the top 100 enriched genes in each tissue type; color bar: blue (low expression), red (high expression), clustering method: K means. (D, E) A tree plot depicting the top GO Biological Processes (GO: BPs) from (B). (F) Bar plot of selected differentially expressed genes between Ncp and Hcp. (G, H). In situ hybridization for selected genes identified in (B).</p

Worksheets containing differential peaks called and annotated by Homer in the wtNcp vs input for histone modifications H3k27Ac, H3k27Me3, H3k4Me3, and H3k4Me1.

Worksheets containing differential peaks called and annotated by Homer in the wtNcp vs input for histone modifications H3k27Ac, H3k27Me3, H3k4Me3, and H3k4Me1.</p

Worksheet containing raw data of DARs identified in Fig 2B, a list of annotated DARs identified in Fig 2B, a comparative analysis of genes associated with DARs and gene expression in wtHcp vs wtNcp related to S1B Fig.

Worksheet containing raw data of DARs identified in Fig 2B, a list of annotated DARs identified in Fig 2B, a comparative analysis of genes associated with DARs and gene expression in wtHcp vs wtNcp related to S1B Fig.</p

Worksheets containing raw data of DARs identified in Fig 4B, a list of annotated DARs identified in Fig 4B, an intersection list of DAR-associated genes that are also occupied by LHX2 in the wtHcp (311 DAGs) identified in Fig 4E.

Worksheets containing raw data of DARs identified in Fig 4B, a list of annotated DARs identified in Fig 4B, an intersection list of DAR-associated genes that are also occupied by LHX2 in the wtHcp (311 DAGs) identified in Fig 4E.</p