Histological, Physiological, and Comparative Proteomic Analyses Provide Insights into Leaf Rolling in <i>Brassica napus</i>

Moderate leaf rolling is important in ideotype breeding, as it improves photosynthetic efficiency and therefore increases crop yields. To understand the regulatory network of leaf rolling in <i>Brassica napus</i>, a down-curved leaf mutant (<i>Bndcl1</i>) has been investigated. Physiological analyses indicated that the chlorophyll contents and antioxidant enzyme activities were remarkably increased and the photosynthetic performance was significantly improved in <i>Bndcl1.</i> Consistent with these findings, 943 differentially accumulated proteins (DAPs) were identified in the <i>Bndcl1</i> mutant and its wild-type plants using iTRAQ-based comparative proteomic analyses. Enrichment analysis of proteins with higher abundance in <i>Bndcl1</i> revealed that the functional category “photosynthesis” was significantly overrepresented. Moreover, proteins associated with oxidative stress response and photosystem II repairing were also up-accumulated in <i>Bndcl1</i>, which might help the mutant to sustain the photosynthetic efficiency under unfavorable conditions. Histological observation showed that the mutant displayed defects in adaxial-abaxial patterning. Important DAPs associated with leaf polarity establishment were detected in <i>Bndcl1</i>, including ribosomal proteins, proteins involved in post-transcriptional gene silencing, and proteins related to brassinosteroid. Together, our findings may help clarify the mechanisms underlying leaf rolling and its physiological effects on plants and may facilitate ideotype breeding in <i>Brassica napus</i>

Histological, Physiological, and Comparative Proteomic Analyses Provide Insights into Leaf Rolling in <i>Brassica napus</i>

Moderate leaf rolling is important in ideotype breeding, as it improves photosynthetic efficiency and therefore increases crop yields. To understand the regulatory network of leaf rolling in <i>Brassica napus</i>, a down-curved leaf mutant (<i>Bndcl1</i>) has been investigated. Physiological analyses indicated that the chlorophyll contents and antioxidant enzyme activities were remarkably increased and the photosynthetic performance was significantly improved in <i>Bndcl1.</i> Consistent with these findings, 943 differentially accumulated proteins (DAPs) were identified in the <i>Bndcl1</i> mutant and its wild-type plants using iTRAQ-based comparative proteomic analyses. Enrichment analysis of proteins with higher abundance in <i>Bndcl1</i> revealed that the functional category “photosynthesis” was significantly overrepresented. Moreover, proteins associated with oxidative stress response and photosystem II repairing were also up-accumulated in <i>Bndcl1</i>, which might help the mutant to sustain the photosynthetic efficiency under unfavorable conditions. Histological observation showed that the mutant displayed defects in adaxial-abaxial patterning. Important DAPs associated with leaf polarity establishment were detected in <i>Bndcl1</i>, including ribosomal proteins, proteins involved in post-transcriptional gene silencing, and proteins related to brassinosteroid. Together, our findings may help clarify the mechanisms underlying leaf rolling and its physiological effects on plants and may facilitate ideotype breeding in <i>Brassica napus</i>

Histological, Physiological, and Comparative Proteomic Analyses Provide Insights into Leaf Rolling in <i>Brassica napus</i>

Moderate leaf rolling is important in ideotype breeding, as it improves photosynthetic efficiency and therefore increases crop yields. To understand the regulatory network of leaf rolling in <i>Brassica napus</i>, a down-curved leaf mutant (<i>Bndcl1</i>) has been investigated. Physiological analyses indicated that the chlorophyll contents and antioxidant enzyme activities were remarkably increased and the photosynthetic performance was significantly improved in <i>Bndcl1.</i> Consistent with these findings, 943 differentially accumulated proteins (DAPs) were identified in the <i>Bndcl1</i> mutant and its wild-type plants using iTRAQ-based comparative proteomic analyses. Enrichment analysis of proteins with higher abundance in <i>Bndcl1</i> revealed that the functional category “photosynthesis” was significantly overrepresented. Moreover, proteins associated with oxidative stress response and photosystem II repairing were also up-accumulated in <i>Bndcl1</i>, which might help the mutant to sustain the photosynthetic efficiency under unfavorable conditions. Histological observation showed that the mutant displayed defects in adaxial-abaxial patterning. Important DAPs associated with leaf polarity establishment were detected in <i>Bndcl1</i>, including ribosomal proteins, proteins involved in post-transcriptional gene silencing, and proteins related to brassinosteroid. Together, our findings may help clarify the mechanisms underlying leaf rolling and its physiological effects on plants and may facilitate ideotype breeding in <i>Brassica napus</i>

Histological, Physiological, and Comparative Proteomic Analyses Provide Insights into Leaf Rolling in <i>Brassica napus</i>

Moderate leaf rolling is important in ideotype breeding, as it improves photosynthetic efficiency and therefore increases crop yields. To understand the regulatory network of leaf rolling in <i>Brassica napus</i>, a down-curved leaf mutant (<i>Bndcl1</i>) has been investigated. Physiological analyses indicated that the chlorophyll contents and antioxidant enzyme activities were remarkably increased and the photosynthetic performance was significantly improved in <i>Bndcl1.</i> Consistent with these findings, 943 differentially accumulated proteins (DAPs) were identified in the <i>Bndcl1</i> mutant and its wild-type plants using iTRAQ-based comparative proteomic analyses. Enrichment analysis of proteins with higher abundance in <i>Bndcl1</i> revealed that the functional category “photosynthesis” was significantly overrepresented. Moreover, proteins associated with oxidative stress response and photosystem II repairing were also up-accumulated in <i>Bndcl1</i>, which might help the mutant to sustain the photosynthetic efficiency under unfavorable conditions. Histological observation showed that the mutant displayed defects in adaxial-abaxial patterning. Important DAPs associated with leaf polarity establishment were detected in <i>Bndcl1</i>, including ribosomal proteins, proteins involved in post-transcriptional gene silencing, and proteins related to brassinosteroid. Together, our findings may help clarify the mechanisms underlying leaf rolling and its physiological effects on plants and may facilitate ideotype breeding in <i>Brassica napus</i>

Histological, Physiological, and Comparative Proteomic Analyses Provide Insights into Leaf Rolling in <i>Brassica napus</i>

Moderate leaf rolling is important in ideotype breeding, as it improves photosynthetic efficiency and therefore increases crop yields. To understand the regulatory network of leaf rolling in <i>Brassica napus</i>, a down-curved leaf mutant (<i>Bndcl1</i>) has been investigated. Physiological analyses indicated that the chlorophyll contents and antioxidant enzyme activities were remarkably increased and the photosynthetic performance was significantly improved in <i>Bndcl1.</i> Consistent with these findings, 943 differentially accumulated proteins (DAPs) were identified in the <i>Bndcl1</i> mutant and its wild-type plants using iTRAQ-based comparative proteomic analyses. Enrichment analysis of proteins with higher abundance in <i>Bndcl1</i> revealed that the functional category “photosynthesis” was significantly overrepresented. Moreover, proteins associated with oxidative stress response and photosystem II repairing were also up-accumulated in <i>Bndcl1</i>, which might help the mutant to sustain the photosynthetic efficiency under unfavorable conditions. Histological observation showed that the mutant displayed defects in adaxial-abaxial patterning. Important DAPs associated with leaf polarity establishment were detected in <i>Bndcl1</i>, including ribosomal proteins, proteins involved in post-transcriptional gene silencing, and proteins related to brassinosteroid. Together, our findings may help clarify the mechanisms underlying leaf rolling and its physiological effects on plants and may facilitate ideotype breeding in <i>Brassica napus</i>

Pleurospermum camtschaticum Hoffm.

原著和名: オホカサモチ科名: セリ科 = Umbelliferae採集地: 青森県 下北郡 東通村 尻屋 (陸奥 下北郡 東通村 尻屋)採集日: 1972/6/11採集者: 萩庭丈壽整理番号: JH004982国立科学博物館整理番号: TNS-VS-95498

Histological, Physiological, and Comparative Proteomic Analyses Provide Insights into Leaf Rolling in <i>Brassica napus</i>

Moderate leaf rolling is important in ideotype breeding, as it improves photosynthetic efficiency and therefore increases crop yields. To understand the regulatory network of leaf rolling in <i>Brassica napus</i>, a down-curved leaf mutant (<i>Bndcl1</i>) has been investigated. Physiological analyses indicated that the chlorophyll contents and antioxidant enzyme activities were remarkably increased and the photosynthetic performance was significantly improved in <i>Bndcl1.</i> Consistent with these findings, 943 differentially accumulated proteins (DAPs) were identified in the <i>Bndcl1</i> mutant and its wild-type plants using iTRAQ-based comparative proteomic analyses. Enrichment analysis of proteins with higher abundance in <i>Bndcl1</i> revealed that the functional category “photosynthesis” was significantly overrepresented. Moreover, proteins associated with oxidative stress response and photosystem II repairing were also up-accumulated in <i>Bndcl1</i>, which might help the mutant to sustain the photosynthetic efficiency under unfavorable conditions. Histological observation showed that the mutant displayed defects in adaxial-abaxial patterning. Important DAPs associated with leaf polarity establishment were detected in <i>Bndcl1</i>, including ribosomal proteins, proteins involved in post-transcriptional gene silencing, and proteins related to brassinosteroid. Together, our findings may help clarify the mechanisms underlying leaf rolling and its physiological effects on plants and may facilitate ideotype breeding in <i>Brassica napus</i>