Integrated transcriptomic and metabolomic data reveal the cold stress responses molecular mechanisms of two coconut varieties

Among tropical fruit trees, coconut holds significant edible and economic importance. The natural growth of coconuts faces a challenge in the form of low temperatures, which is a crucial factor among adverse environmental stresses impacting their geographical distribution. Hence, it is essential to enhance our comprehension of the molecular mechanisms through which cold stress influences various coconut varieties. We employed analyses of leaf growth morphology and physiological traits to examine how coconuts respond to low temperatures over 2-hour, 8-hour, 2-day, and 7-day intervals. Additionally, we performed transcriptome and metabolome analyses to identify the molecular and physiological shifts in two coconut varieties displaying distinct sensitivities to the cold stress. As the length of cold stress extended, there was a prominent escalation within the soluble protein (SP), proline (Pro) concentrations, the activity of peroxidase (POD) and superoxide dismutase (SOD) in the leaves. Contrariwise, the activity of glutathione peroxidase (GSH) underwent a substantial reduction during this period. The widespread analysis of metabolome and transcriptome disclosed a nexus of genes and metabolites intricately cold stress were chiefly involved in pathways centered around amino acid, flavonoid, carbohydrate and lipid metabolism. We perceived several stress-responsive metabolites, such as flavonoids, carbohydrates, lipids, and amino acids, which unveiled considerably, lower in the genotype subtle to cold stress. Furthermore, we uncovered pivotal genes in the amino acid biosynthesis, antioxidant system and flavonoid biosynthesis pathway that presented down-regulation in coconut varieties sensitive to cold stress. This study broadly enriches our contemporary perception of the molecular machinery that contributes to altering levels of cold stress tolerance amid coconut genotypes. It also unlocks several unique prospects for exploration in the areas of breeding or engineering, aiming to identifying tolerant and/or sensitive coconut varieties encompassing multi-omics layers in response to cold stress conditions

Antihypertensive effect of giant embryo brown rice and pre‐germinated giant embryo brown rice on spontaneously hypertensive rats

“Shangshida NO.5” is a giant embryo mutant resulting from giant embryo gene (GE) dysfunction in “Chao2‐10” rice. Here, we compared the antihypertensive effects of “Chao2‐10” brown rice (C2‐10), “Shangshida NO.5” brown rice (GER), and pre‐germinated “Shangshida NO.5” brown rice (PGER) in spontaneously hypertensive rats (SHR). Male SHR at 6 weeks of age were divided into four groups and were fed with (a) a control diet (control), (b) a 40% C2‐10‐supplemented diet (C2‐10), (c) a 40% GER‐supplemented diet (GER), or (d) a 40% PGER‐supplemented diet (PGER) for 8 weeks, and their physiological and biochemical parameters were measured. The results showed that the C2‐10‐, GER‐, and PGER‐supplemented diets significantly decreased systolic blood pressure (SBP) and diastolic blood pressure (DBP) during the experiment. At the end of the experimental period, the SBP and DBP of the C2‐10, GER, and PGER groups were 7.6, 23.3, and 31.1 mmHg and 9.8, 21.1, and 29 mmHg lower than those in the control group, respectively, suggesting the GER and PGER diets were better able to inhibit blood pressure elevation than the C2‐10 diet. The serum creatinine levels in the C2‐10, GER, and PGER groups and the blood urea nitrogen content in the PGER group were significantly lower than those of the control group, indicating that C2‐10‐, GER‐, and especially PGER‐supplemented diets improved renal function. In addition, the antioxidant activity of the C2‐10 group and especially of the GER and PGER groups also improved. The above results suggest that “Shangshida NO.5” rice, particularly pre‐germinated rice, is a good dietary supplement for preventing the development of hypertension

Development of aromatic soft rice “Shangshida No.18” by molecular marker-assisted selection

In this study,we developed a new rice strain,"Shangshida No.18",by molecular marker-assisted gene pyramiding,using aromatic and soft rice "Qingxiangruanjing" as the parent donor of soft rice gene and using aromatic and non-soft rice "Guangmingjing No.2" as a backcross parent.The growth duration of developed "Shangshida No.18" rice was about 148 days,5 days shorter than that of "Qingxiangruanjing" rice,and there were no significant difference in agronomic and yield traits of "Shangshida No.18" rice and "Guangmingjing No.2"rice.Amylose content of "Shangshida No.18" rice was 8.2%,which belongs to range of soft rice.The successful development of the new rice line "Shangshida No.18" has laid a solid foundation for the further production of aromatic and soft rice with shorter growth duration in Shanghai

CircSRSF4 Enhances Proliferation, Invasion, and Migration to Promote the Progression of Osteosarcoma via Rac1

(1) Background: At present, cancer cell metastasis is the main cause of death in patients with malignant tumors, and up to 23% of osteosarcoma patients have died due to lung and lymph node metastasis. Therefore, finding new molecules involved in tumor development can provide new strategies for the diagnosis and treatment of osteosarcoma patients. Circular RNAs (circRNAs) are a type of RNA molecule that are connected head-to-tail to form a closed ring. There is increasing evidence that circRNAs are RNA molecules with many biological functions in various diseases. However, the role and mechanism of circRNAs in osteosarcoma have rarely been reported. (2) Methods: The expression of circSRSF4 in osteosarcoma tissues and cell lines was detected by quantitative real-time PCR (RT-qPCR), and the result of high-throughput sequencing was verified. In order to explore the effect of circSRSF4 on tumor proliferation, invasion, and migration, a dual-luciferase reporter assay, RNA binding protein immunoprecipitation assay, cell counting kit-8 (CCK-8), transwell assay, scratch wound healing assay, Western blot analysis, and other experiments were carried out in vitro. Rescue experiments and a xenograft model confirmed that circSRSF4 directly acted on miR-224 to regulate Rac1 expression. (3) Results: The expression of circSRSF4 was significantly higher in osteosarcoma tissues and cell lines. Down-regulating the expression of circSRSF4 in vitro significantly inhibited the proliferation, invasion, and migration of cells, and also reduced the expression of Rac1, while the overexpression of Rac1 and miR-224 inhibition could reverse these effects. The inhibition of circSRSF4 expression in vivo also attenuated tumor growth. A mechanistic study showed that circSRSF4 can be used as an miR-224 sponge to up-regulate the expression of Rac1, thereby promoting the development of osteosarcoma. (4) Conclusions: CircSRSF4 acting as a ceRNA promotes the malignant behavior of osteosarcoma through the circSRSF4/miR-224/Rac1 axis, which provides a new theoretical basis for the clinical prevention and treatment of osteosarcoma and the study of related markers and intervention targets

Spatiotemporal Characteristics of Drought in Central Asia from 1981 to 2020

Drought is a meteorological phenomenon that threatens ecosystems, agricultural production, and living conditions. Central Asia is highly vulnerable to drought due to its special geographic location, water resource shortages, and extreme weather conditions, and poor management of water resources and reliance on irrigated agriculture exacerbate the effects of drought. In this study, the latest version of the Global Land Data Assimilation System was employed to calculate the Standardized Precipitation Evapotranspiration Index at different time scales during the period from 1981 to 2020. The varimax Rotated Empirical Orthogonal Function was applied for subregional delineation of drought patterns in Central Asia, and various methods were employed for a comparative analysis of the spatiotemporal characteristics of drought in these Central Asian subregions. The results show that drought patterns vary considerably in the Central Asian subregions. Over the past 40 years, alternating wet and dry conditions occurred in Central Asia. North Kazakhstan experienced more drought events with lower severity. East and west differences appear after 2001, the west becoming drier and the east becoming wetter. Some regions near lakes, such as Balkhash, Issyk-Kul, and the Aral Sea, suffer from droughts of long duration and high severity. In the Tianshan region, droughts in the northern slopes occur more frequently, with shorter durations and higher intensity and peaks. Northwestern China and western Mongolia have extensive agricultural land and grasslands with highly fragile ecosystems that have become progressively drier since 2001

Consumption frequencies of beverages and the hypertension risk in adults: a cohort study in China

Objective To explore the associations between the consumption frequencies of alcohol, tea and sugar-sweetened beverages (SSBs) and the hypertension risk among Chinese adults.Design A longitudinal study of the effect of beverage consumption on hypertension risk.Setting Nine provinces in China, including Jiangsu, Hubei, Hunan, Guangxi, Guizhou, Liaoning, Heilongjiang, Shandong and Henan.Participants The longitudinal data of the China Health and Nutrition Survey from 2004 to 2015 were used. A total of 4427 participants from 9 provinces were included at baseline.Outcome First incidence of hypertension.Results During a mean follow-up of 8.7 years, 1478 participants developed hypertension. Alcohol consumption more than twice a week in young men (HR 1.86, 95% CI 1.09 to 3.18) or middle-aged men (HR 1.37, 95% CI 1.01 to 1.87) was associated with a higher hypertension risk. Middle-aged women who consumed tea frequently (HR 0.71, 95% CI 0.52 to 0.97), or young women who consumed SSBs less than once a week (HR 0.31, 95% CI 0.14 to 0.67) had a lower risk of hypertension.Conclusions High-frequency alcohol consumption increased the risk of hypertension in men, and frequent tea consumption and low-frequency SSBs consumption were associated with lower risk of hypertension in women. Consumption frequency of beverages was also suggested to be considered in the prevention and control of hypertension

Interaction effect between overweight/obesity and alcohol consumption on hypertension risk in China: a longitudinal study

Objective To explore the interaction effect between overweight/obesity and alcohol consumption on hypertension risk.Design A longitudinal study of the independent and combined effects of hypertension risk factors.Setting Twelve provinces in China, including Beijing Liaoning, Heilongjiang, Shanghai, Jiangsu, Shandong, Henan, Hubei, Hunan, Guangxi, Guizhou and Chongqing.Participants Longitudinal data of China Health and Nutrition Survey, collected between 2011 and 2015, were used in this study. A total of 13 121 residents from 12 provinces were included and completed physical examinations and questionnaires at baseline.Outcome First incidence of hypertension.Results Over a mean follow-up of 4 years, 690 incident hypertension cases were reported. After adjusting for age, gender, education level, marital status, physical activity, diabetes and smoking, high body mass index (BMI) and light drinking (OR=5.07, 95% CI 3.06 to 8.41), high waist circumference (WC) and light drinking (OR=4.81, 95% CI 2.92 to 7.91), high waist hip ratio and light drinking (OR=2.85, 95% CI 1.84 to 4.42) were the highest risk of all participants in the three combinations. Multiplicative interaction measures were statistically significant in overweight/obesity and drinking/light drinking/heavy drinking categories in men (p<0.05). Additive interactions were observed between high BMI and drinking in men (relative excess risk due to interaction=1.75, 95% CI 0.85 to 2.65, attributable proportion due to interaction=0.56, 95% CI 0.36 to 0.76, synergy index=6.43, 95% CI 1.02 to 28.84).Conclusions Measures of body weight and size, particularly BMI and WC, appear to interact synergistically with alcohol consumption to increase the risk of hypertension in the Chinese population. Given that approximately 245 million people in China have hypertension, and that hypertension is a major cause of cardiovascular disease worldwide, our results may have implications for chronic disease prevention

Engineered biochar derived from lemon peel waste for highly efficient removal of organic pollutants from water

The urgent need for efficient decontamination of organic dyes from polluted waters necessitates the search for a low-cost and suitable adsorbent. The large amount of lemon peel residue in actual production can be an ideal raw material for the preparation of adsorbents. Herein, a novel magnetic nanocomposite derived from lemon (Citrus limon L.) peel residue (Fe3O4/Lp-biochar) was successfully synthesized by one-step hydrothermal method and used for the adsorptive capture of methylene blue (MB) in aqueous solution. The prepared Fe3O4/Lp-biochar are spherical with a particle size of 8.3 nm and have a saturation magnetization intensity of 52.9 emu/g and a specific surface area of 64.30 m2/g. It was found that the maximum MB adsorption capacity of 26.36 mg/g by the Fe3O4/Lp-biochar can be achieved when the amount of nanocomposite was 0.10 mg/mL, the system's pH value was 10, the reaction temperature was 25 °C, and the adsorption time was 60 min. Adsorption kinetics of MB on the Fe3O4/Lp-biochar followed the pseudo-second-order kinetic model whilst the Freundlich isotherm model could well simulate the adsorption behavior of MB on magnetic biochar. The adsorption mechanism of MB by the Fe3O4/Lp-biochar predominantly include electrostatic attraction and mesoporous interaction. Furthermore, the Fe3O4/Lp-biochar exhibited a remarkable retention of 94.7% of the initial adsorption efficiency even after the 10th cycle of repeated use. It was concluded that the lemon peel-derived magnetic biochar can be a promising alternative to conventional adsorbents for wastewater treatment

Circular RNA F-circEA-2a derived from EML4-ALK fusion gene promotes cell migration and invasion in non-small cell lung cancer

Abstract Oncogenic fusion gene Echinoderm Microtubule-associated protein-Like 4-Anaplastic Lymphoma Kinase (EML4-ALK) contributes to tumorigenesis of a subset of non-small cell lung cancer (NSCLC). Recently, we demonstrated that F-circEA-4a, a tumor-promoting circular RNA (circRNA) generated from the back-splicing of EML4-ALK variant 3b (v3b), is a novel liquid biopsy biomarker for NSCLC. However, circRNAs produced from EML4-ALK gene and their roles in NSCLC are not well-characterized. Here, we identify another EML4-ALK-v3b-derived circRNA, F-circEA-2a, harboring “AA” (rather than “AAAA” in F-circEA-4a) motif at the junction site. F-circEA-2a mainly locates in the cytoplasm and promotes cell migration and invasion, but has little effect on cell proliferation. Moreover, F-circEA-2a exists in tumor, but not in the plasma of NSCLC patients with EML4-ALK fusion gene, further supporting the significant diagnostic value of F-circEA-4a for EML4-ALK-positive NSCLC. This work finds a novel oncogenic circRNA generated from EML4-ALK fusion gene, highlighting the pivotal role of circRNA in EML4-ALK-positive NSCLC development

Coral reef applications of Landsat-8: geomorphic zonation and benthic habitat mapping of Xisha Islands, China

Being one of the most significant and valuable coral reef systems in the South China Sea, the Xisha Islands has undergone rapid transformation due to increasing stressors from human impacts and climate change in recent years. However, as indispensable information for coral reef monitoring and management, the detailed reef extent, geomorphic zonation, or benthic composition of the Xisha Islands is not well documented. Considering limited access to the Xisha Islands, the rapid development of optical remote sensing technology provides us with a feasible mean for coral reef observation. This study adopted a water depth substitution index – probabilistic inundation (PI) – combined with depth-invariant index (DII) to achieve reef extent exploration, geomorphologic and benthic habitat types classification with unsupervised classification algorithms based on Landsat-8 time-series satellite data. Compared with two open-access datasets, the extent of each independent reef extracted from PI exhibited higher similarity with the actual boundary conditions displayed in RGB (Red-Green-Blue) composite images from Landsat-8. Based on PI and derived slope, we obtained geomorphic zonation classification results, and similarly benthic compositions were retrieved based on PI, DII, and reflectance. The overall accuracy of geomorphic zonation and benthic habitat classification results were 72% and 86%, respectively. We also interestingly discovered that corals of the Xisha Islands may be capable of an ability to resist chronic heat stress as a growth trend of reef area after two successive stress events in 2014–2015 were observed at most reefs. The proposed mapping framework of this study provides a repeatable and flexible scheme in depicting the comprehensive situation of coral reefs at Xisha Islands based only on publicly available remote sensing data without complicated pre-set parameters, which could be easily extended to coral reef research around the world. Simultaneously, the findings also provide requisite information supporting the sustainable management and conservation of coral reef ecosystems in the Xisha Islands.</p