Ligustrazine Inhibits the Migration and Invasion of Renal Cell Carcinoma

Ligustrazine is a Chinese herb (Chuanxiong) approved for use as a medical drug in China. Recent evidence suggests that ligustrazine has promising antitumor properties. Our preliminary results showed that ligustrazine could inhibit the growth of human renal cell carcinoma (RCC) cell lines. However, the complicated molecular mechanism has not been fully revealed. Therefore, the purpose of this study to investigate the mechanism of ligustrazine resistance in human RCC cells. Cell proliferation, migration, invasion, and colony-formation ability of RCC cells A498 were detected by MTT assay, clonal formation rates, and transwell chamber assay in vitro. The expression of epithelial–mesenchymal transition (EMT)–related proteins were analyzed using western blot test. The effect of ligustrazine on the growth of A498 cells in nude mice was investigated in vivo. Our results showed that ligustrazine could significantly inhibit the proliferation, migration, and invasion of A498 both in vivo and vitro. Western blot analysis showed that the expressions of EMT-related, N-cadherin, snail, and slug proteins were significantly decreased in A498 in the ligustrazine treatment group. This study indicated that ligustrazine could significantly inhibit the malignant biological behaviors of RCC cell lines, possibly by inhibiting the EMT process

Ligustrazine Inhibits the Migration and Invasion of Renal Cell Carcinoma

Ligustrazine is a Chinese herb (Chuanxiong) approved for use as a medical drug in China. Recent evidence suggests that ligustrazine has promising antitumor properties. Our preliminary results showed that ligustrazine could inhibit the growth of human renal cell carcinoma (RCC) cell lines. However, the complicated molecular mechanism has not been fully revealed. Therefore, the purpose of this study to investigate the mechanism of ligustrazine resistance in human RCC cells. Cell proliferation, migration, invasion, and colony-formation ability of RCC cells A498 were detected by MTT assay, clonal formation rates, and transwell chamber assay in vitro. The expression of epithelial–mesenchymal transition (EMT)–related proteins were analyzed using western blot test. The effect of ligustrazine on the growth of A498 cells in nude mice was investigated in vivo. Our results showed that ligustrazine could significantly inhibit the proliferation, migration, and invasion of A498 both in vivo and vitro. Western blot analysis showed that the expressions of EMT-related, N-cadherin, snail, and slug proteins were significantly decreased in A498 in the ligustrazine treatment group. This study indicated that ligustrazine could significantly inhibit the malignant biological behaviors of RCC cell lines, possibly by inhibiting the EMT process

mechanismofdichloromethanedisproportionationovermesoporoustio2underlowtemperature

Mesoporous TiO2 was synthesized via nonhydrolytic template-mediated sol-gel route.Catalytic degradation performance upon dichloromethane over as-prepared mesoporous TiO2,pure anatase and rutile were investigated respectively.Disproportionation took place over as-made mesoporous TiO2 and pure anatase under the presence of water.The mechanism of disproportionation was studied by in situ FTIR.The interaction between chloromethoxy species and bridge coordinated methylenes was the key step of disproportionation.Formate species and methoxy groups would be formed and further turned into carbon monoxide and methyl chloride.Anatase(001)played an important role for disproportionation in that water could be dissociated into surface hydroxyl groups on such structure.As a result,the consumed hydroxyl groups would be replenished.In addition,there was another competitive oxidation route governed by free hydroxyl radicals.In this route,chloromethoxy groups would be oxidized into formate species by hydroxyl radicals transfering from the surface of TiO2.The latter route would be more favorable at higher temperature

mechanismofdichloromethanedisproportionationovermesoporoustio2underlowtemperature

Mesoporous tio2 was synthesized via nonhydrolytic template-mediated sol-gel route. catalytic degradation performance upon dichloromethane over as-prepared mesoporous tio2, pure anatase and rutile were investigated respectively. disproportionation took place over as-made mesoporous tio2 and pure anatase under the presence of water. the mechanism of disproportionation was studied by in situ ftir. the interaction between chloromethoxy species and bridge coordinated methylenes was the key step of disproportionation. formate species and methoxy groups would be formed and further turned into carbon monoxide and methyl chloride. anatase (001) played an important role for disproportionation in that water could be dissociated into surface hydroxyl groups on such structure. as a result, the consumed hydroxyl groups would be replenished. in addition, there was another competitive oxidation route governed by free hydroxyl radicals. in this route, chloromethoxy groups would be oxidized into formate species by hydroxyl radicals transfering from the surface of tio2. the latter route would be more favorable at higher temperature. (c) higher education press and springer-verlag gmbh germany, part of springer nature 201

mechanismofdichloromethanedisproportionationovermesoporoustio2underlowtemperature

Mesoporous TiO2 was synthesized via nonhydrolytic template-mediated sol-gel route.Catalytic degradation performance upon dichloromethane over as-prepared mesoporous TiO2,pure anatase and rutile were investigated respectively.Disproportionation took place over as-made mesoporous TiO2 and pure anatase under the presence of water.The mechanism of disproportionation was studied by in situ FTIR.The interaction between chloromethoxy species and bridge coordinated methylenes was the key step of disproportionation.Formate species and methoxy groups would be formed and further turned into carbon monoxide and methyl chloride.Anatase(001)played an important role for disproportionation in that water could be dissociated into surface hydroxyl groups on such structure.As a result,the consumed hydroxyl groups would be replenished.In addition,there was another competitive oxidation route governed by free hydroxyl radicals.In this route,chloromethoxy groups would be oxidized into formate species by hydroxyl radicals transfering from the surface of TiO2.The latter route would be more favorable at higher temperature

Promoted VOC oxidation over homogeneous porous CoxNiAlO composite oxides derived from hydrotalcites: effect of preparation method and doping

Homogeneous porous and curve plated CoxNiAlO composite metal oxide catalysts are obtained from the thermal decomposition of CoxNiAl-layered double hydroxide (LDH) precursors, which are prepared by urea co-precipitation with surfactant, followed by a hydrothermal treatment. The as-prepared samples were characterized by XRD, BET, SEM, TEM, H-2-TPR and XPS. The Co3AlO sample shows 90% benzene conversion (T-90) at 236 degrees C at a high space velocity (SV = 60 000 mL g(-1) h(-1)), and possesses much higher activity than Co3AlO prepared with NaOH co-precipitation without surfactant, with T-90 = 288 degrees C. This is mainly correlated with the narrower pore size (2.9 vs. 17.2 nm) and lower temperature reducibility (319 vs. 360 degrees C). The Co2NiAlO sample exhibits enhanced activity at T-90 = 227 degrees C with the low activation energy of 39.0 kJ mol(-1), and its lower temperature reducibility is ascribed to the larger amount of surface accessible Co3+. The Co2NiAlO sample owns good reproducibility and superior reversibility and long stability with prolonged time on benzene stream in the presence of 3.5% water vapor. Moreover, a monolithic Co2NiAlO film catalyst is fabricated by the thermal decomposition of an LDH film precursor through an in situ growth methodology, with a high reaction rate of 1.21 mmol g(-1) h(-1) under T-90 = 275 degrees C

Mechanism of dichloromethane disproportionation over mesoporous TiO2 under low temperature

Mesoporous TiO2 was synthesized via nonhydrolytic template-mediated sol-gel route. Catalytic degradation performance upon dichloromethane over as-prepared mesoporous TiO2, pure anatase and rutile were investigated respectively. Disproportionation took place over as-made mesoporous TiO2 and pure anatase under the presence of water. The mechanism of disproportionation was studied by in situ FTIR. The interaction between chloromethoxy species and bridge coordinated methylenes was the key step of disproportionation. Formate species and methoxy groups would be formed and further turned into carbon monoxide and methyl chloride. Anatase (001) played an important role for disproportionation in that water could be dissociated into surface hydroxyl groups on such structure. As a result, the consumed hydroxyl groups would be replenished. In addition, there was another competitive oxidation route governed by free hydroxyl radicals. In this route, chloromethoxy groups would be oxidized into formate species by hydroxyl radicals transfering from the surface of TiO2. The latter route would be more favorable at higher temperature. (c) Higher Education Press and Springer-Verlag GmbH Germany, part of Springer Nature 201

Mater. Lett.

A low-temperature sol-gel process associated with different surfactants in ethanol solvent was applied to prepare ultrafine MnO2 nanowires and nanorods. The surfactants used in the synthesis procedure are crucial for the structure of products. Highly dispersed MnO2 nanowires was formed by assistance of cetyltrimethyl ammonium bromide while hierarchical particles assembled with nanowires were synthesized by usage of pluronic P123 triblock copolymer or Polyvinyl pyrrolidone. The free-standing sheet like structure aggregated with nanorods was obtained by using sodium dodecyl sulfate as a surfactant. All samples were characterized by SEM, TEM, XRD and the results indicated these MnO2 ultrafine nanostructures could be expediently produced by this facile method. (C) 2014 Elsevier B.V. All rights reserved.A low-temperature sol-gel process associated with different surfactants in ethanol solvent was applied to prepare ultrafine MnO2 nanowires and nanorods. The surfactants used in the synthesis procedure are crucial for the structure of products. Highly dispersed MnO2 nanowires was formed by assistance of cetyltrimethyl ammonium bromide while hierarchical particles assembled with nanowires were synthesized by usage of pluronic P123 triblock copolymer or Polyvinyl pyrrolidone. The free-standing sheet like structure aggregated with nanorods was obtained by using sodium dodecyl sulfate as a surfactant. All samples were characterized by SEM, TEM, XRD and the results indicated these MnO2 ultrafine nanostructures could be expediently produced by this facile method. (C) 2014 Elsevier B.V. All rights reserved

Fabrication of silica supported Mn-Ce benzene oxidation catalyst by a simple and environment-friendly oxalate approach

A series of silica supported Mn-Ce composite oxides with different Mn/Ce molar ratios were obtained by a simple and environment-friendly oxalate route. The physical and chemical properties were characterized by TG, BET, SEM, TEM, XPS and TPR analysis. All catalysts showed excellent activity towards deep oxidation of benzene. The effects of Mn/Ce ratio, calcination temperature on the structure and catalytic activity of catalysts were investigated. Catalyst from nitrate precursor was also characterized to compare the influence of different precursors. The 6Mn4Ce sample from oxalate route sintered at 400 A degrees C showed the maximum reaction rate of 0.50 mmol gcat(-1)h(-1); T90 of the catalyst is 216 A degrees C. The catalytic activity is related to surface area, pore size distribution, surface elemental species, particle size distribution and low temperature reducibility which may derived from synergistic effect between manganese and cerium oxide. Compared with nitrate precursors, catalyst from oxalate route can be more finely dispersed on the pores of silica without damaging the pore structure of support. The role of silica is not only a support, but also an in situ reaction site for precursor's decomposition, which ensure the finely distribution of active components. In addition, the best catalyst showed good stability with prolonged time on stream