The Research on the Model of the Multi-Interval Fracturing of Horizontal Wells

Multi-interval fracturing technology in horizontal well are used more and more widely in the exploitation of unconventional hydrocarbon resources, to accurately determine the initiation pressure having an important significance to improve the fracturing effect. On the basis of the homogeneous and isotropic two-dimensional plane strain model, established the geometric model of two-dimensional vertical fractures induced stress field; Based on rock mechanics and elastic mechanics theory, taking multi-stage fracturing induced stress into consideration, established the calculation model of the initiation pressure of the multi-stage fracturing of horizontal wells’ fracture. The calculation results showed that taking induced stress into consideration, the calculation result is consistent with the actual situation, compared with the actual construction pressure, and the average accuracy rate is 95.15%. It proved that this model can be used for calculating the initiation pressure of the multi-interval fracturing of horizontal wells’ fracture.Key words: Horizontal well; Multistage fracture; Initiation pressure; Induced stres

Experimental investigation of rotordynamic coefficients for the labyrinth seals with and without shunt injection

Shunt injection serves an important role in the labyrinth seal static and rotordynamic characteristics which are important in the prediction of turbomachinery stability. This paper analyzed how the shunt injection affects the seal rotordynamic characteristics, and presented an improved impedance method based on unbalanced synchronous excitation to identify the rotordynamic coefficients of labyrinth seals on a rotor test rig. The influences of the rotational speed and the inlet/outlet pressure ratio on the rotordynamic characteristics of shunt injection seals with and without shunt injection were identified and analyzed. The experimental results reveal that all the seal rotordynamic coefficients increase with the rotational speed, and the inlet/outlet pressure ratio. The shunt injection contributes to decreasing the seal cross-coupled stiffness, and increasing the direct damping. The shunt injection plays an important role in decreasing the effective stiffness coefficient, and increasing the effective damping coefficient. The shunt injection can effectively improve the rotor stability. The experimental results lay the foundation for designing the annular seals with shunt injection

Mutagenesis of the fusion peptide-like domain of hepatitis C virus E1 glycoprotein: involvement in cell fusion and virus entry

<p>Abstract</p> <p>Background</p> <p>Envelope (E) glycoprotein E2 of the hepatitis C virus (HCV) mediates binding of the virus to target cell receptors. Nevertheless, the precise role of E1 in viral entry remains elusive.</p> <p>Methods</p> <p>To understand the involvement of the fusion peptide-like domain positioned at residues 264 to 290 within envelope glycoprotein E1 in HCV infection, mutants with Ala and Asn substitutions for residues conserved between HCV and E proteins of flaviviruses or the fusion proteins of paramyxoviruses were constructed by site-directed mutagenesis and their effects on membrane fusion and viral infectivity were examined.</p> <p>Results</p> <p>None of these mutations affected the synthesis or cell surface expression of envelope proteins, nor did they alter the formation of a non-covalent E1-E2 heterodimer or E2 binding to the large extracellular loop of CD81. The Cys residues located at positions 272 and 281 were unlikely involved in intra- or intermolecular disulfide bond formation. With the exception of the G267A mutant, which showed increased cell fusion, other mutants displayed reduced or marginally inhibited cell fusion capacities compared to the wild-type (WT) E1E2. The G267A mutant was also an exception in human immunodeficiency virus type 1 (HIV-1)/HCV E1E2 pseudotyping analyses, in that it showed higher one-cycle infectivity; all other mutants exhibited greatly or partially reduced viral entry versus the WT pseudotype. All but the G278A and D279N mutants showed a WT-like profile of E1E2 incorporation into HIV-1 particles. Since C272A, C281A, G282A, and G288A pseudotypes bound to Huh7 cells as effectively as did the WT pseudotype, the reduced infectivity of these pseudotypes was due to their ability to inhibit cell fusion.</p> <p>Conclusion</p> <p>Our results indicate that specific residues, but not the structure, of this fusion peptide-like domain are required for mediating cell fusion and viral entry.</p

Synchronization of General Complex Networks with Hybrid Couplings and Unknown Perturbations

The issue of synchronization for a class of hybrid coupled complex networks with mixed delays (discrete delays and distributed delays) and unknown nonstochastic external perturbations is studied. The perturbations do not disappear even after all the dynamical nodes have reached synchronization. To overcome the bad effects of such perturbations, a simple but all-powerful robust adaptive controller is designed to synchronize the complex networks even without knowing a priori the functions and bounds of the perturbations. Based on Lyapunov stability theory, integral inequality Barbalat lemma, and Schur Complement lemma, rigorous proofs are given for synchronization of the complex networks. Numerical simulations verify the effectiveness of the new robust adaptive controller

Formation of meso, N-diphenylprotoporphyrin IX by an aerobic reaction of phenylhydrazine with oxyhemoglobins.

Administration of phenylhydrazine to rabbits resulted in the denaturation of hemoglobins in erythrocytes, causing the formation of intracellular precipitates known as Heinz bodies, severe hemolytic anemia, and reticulocytosis. To elucidate the molecular mechanism of the destabilization, we allowed human oxyhemoglobins to react aerobically with phenylhydrazine. After treatment with acetic acid/HCl and H2SO4/methanol, the chloroform extract contained blue-green pigments of major products accompanied by different minor products. Each product was isolated by column chromatography. By fast-atom-bombardment mass spectrometry (FAB-MS) and proton nuclear magnetic resonance (1H-NMR) spectrometry, dimethyl esters of N-phenylprotoporphyrin IX and meso, N-diphenylprotoporphyrin IX were determined. Other major products also were determined to be dimethyl esters of triphenyl-and tetraphenyl-substituted protoporphyrins by FAB-MS. The formation of meso, N-diphenylprotoporphyrin indicated that the addition of a phenyl radical to the meso-carbon atom of the protoporphyrin ring occurred. Triphenyl and tetraphenyl adducts also indicated the formation of phenyl radicals in the aerobic reaction of phenylhydrazine with oxyhemoglobins. From these results, we suggest that the formation of phenyl radicals and the replacement of heme with phenyl-substituted protoporphyrins cause the destabilization of hemoglobins to induce Heinz bodies and hemolytic anemia with phenylhydrazine.</p

The research of pipeline lifting model in horizontal directional drilling

In recent years, Horizontal Directional Drilling has been the first choice in trenchless engineering of pipeline crossing for its strong environmental adaptability, high efficiency and low cost. In order to reduce the resistance force and avoid pipeline damage in construction, the angle formed by the pipeline axis and hole axis should be lessened when the pipeline is lifted to a height. However, the stress status of the pipeline is very complex during the lifting process. Hence the research of pipeline lifting process is great importance for pipeline safety. In this paper, a finite element model is established to uncover the stress variety law of pipeline during lifting process. And then its reliability was verified by the experimental method. At last, the results of engineering experiment show that the finite element model which is credible can be used to reveal the stress variety law of the pipeline during the lifting process