3 research outputs found
Iron-Doped MetalāZinc-Centered Organic Framework Mesoporous Carbon Derivatives for Single-Wavelength NIR-Activated Photothermal/Photodynamic Synergistic Therapy
Recently, single-wavelength synergetic photothermal/photodynamic
(PTT/PDT) therapy is beginning to make its mark in cancer treatment,
and the key to it is a photosensitizer. In this work, an iron-doped
metalāzinc-centered organic framework mesoporous carbon derivative
(denoted as Fex-Zn-NCT) with
a similar porphyrin property was successfully synthesized by a mild,
simple, and green aqueous reaction. The effects of different Fe contents
and pyrolysis temperatures on the morphology, structure, and PTT/PDT
of Fex-Zn-NCT were investigated.
Most importantly, we found that Fe50-Zn-NC900 exhibited excellent PTT/PDT performance under single-wavelength
near-infrared (808 nm) light irradiation in a hydrophilic environment.
The photothermal conversion efficiency (Ī·) was counted as ā¼81.3%,
and the singlet oxygen (1O2) quantum yield (Ī¦)
was compared with indocyanine green (ICG) as ā¼0.0041. Furthermore,
Fe50-Zn-NC900 is provided with a clear ability
for generating 1O2 in living tumor cells and
inducted massive necrosis/apoptosis of tumor cells with single-wavelength
near-infrared laser irradiation. All of these are clear to consider
that Fe50-Zn-NC900 displays great potential
as an excellent photosensitizer for single-wavelength dual-mode PTT/PDT
therapy
Constructing Higher-Order DNA Nanoarchitectures with Highly Purified DNA Nanocages
DNA nanostructures have attracted
great attention due to their precisely controllable geometry and great
potential in various areas including bottom-up self-assembly. However,
construction of higher-order DNA nanoarchitectures with individual
DNA nanostructures is often hampered with the purity and quantity
of these ābricksā. Here, we introduced size exclusion
chromatography (SEC) to prepare highly purified tetrahedral DNA nanocages
in large scale and demonstrated that precise quantification of DNA
nanocages was the key to the formation of higher-order DNA nanoarchitectures.
We successfully purified a series of DNA nanocages with different
sizes, including seven DNA tetrahedra with different edge lengths
(7, 10, 13, 17, 20, 26, 30 bp) and one trigonal bipyramid with a 20-bp
edge. These highly purified and aggregation-free DNA nanocages could
be self-assembled into higher-order DNA nanoarchitectures with extraordinarily
high yields (98% for dimer and 95% for trimer). As a comparison, unpurified
DNA nanocages resulted in low yield of 14% for dimer and 12% for trimer,
respectively. AFM images cleraly presented the characteristic structure
of monomer, dimer and trimer, impling the purified DNA nanocages well-formed
the designed nanoarchitectures. Therefore, we have demonstrated that
highly purified DNA nanocages are excellent ābricksā
for DNA nanotechnology and show great potential in various applications
of DNA nanomaterials
Preservation of DNA Nanostructure Carriers: Effects of FreezeāThawing and Ionic Strength during Lyophilization and Storage
DNA nanostructures have attracted
wide interest in biomedical applications,
especially as nanocarriers for drug delivery. Therefore, it is important
to ensure the structural integrity of DNA nanostructures under ambient
temperature storage. In this study, we examined lyophilization-based
preservation of DNA nanostructures by investigating the structural
integrity of different DNA nanostructures reconstituted from lyophilization.
We demonstrated that lyophilization under appropriate ionic strength
is amenable to the storage of DNA nanostructures. Compared with that
stored in liquid solution, DNA nanostructure carriers reconstituted
from lyophilization showed significantly better structural integrity
after an accelerated aging test equivalent to 100-day room-temperature
storage