Distinct Frontal Ablation Processes Drive Heterogeneous Submarine Terminus Morphology

An edited version of this paper was published by AGU. Copyright 2019 American Geophysical Union.Calving and submarine melt drive frontal ablation and sculpt the ice face of marine‐terminating glaciers. However, there are sparse observations of submarine termini, which limit estimates of spatially varying submarine melt. Here we present a detailed survey of a west Greenland glacier to reveal heterogeneity in submarine terminus morphology. We find that the majority of the terminus (~77%) is undercut, driven by calving in the upper water column and submarine melting at depth. The remaining ~23% of the terminus is overcut, driven by calving alone. We use observations of six subglacial discharge outlets, combined with a plume model, to estimate spatially varying discharge fluxes. While small discharge fluxes (<43 m3/s) feed numerous, deeply undercut outlets with subsurface plumes, ~70% of the net subglacial flux emerges at the terminus center, producing a vigorous, surface‐reaching plume. This primary outlet drives large, localized seasonal retreat that exceeds calving rates at secondary outlets

Distributed subglacial discharge drives significant submarine melt at a Greenland tidewater glacier

Submarine melt can account for substantial mass loss at tidewater glacier termini. However, the processes controlling submarine melt are poorly understood due to limited observations of submarine termini. Here at a tidewater glacier in central West Greenland, we identify subglacial discharge outlets and infer submarine melt across the terminus using direct observations of the submarine terminus face. We find extensive melting associated with small discharge outlets. While the majority of discharge is routed to a single, large channel, outlets not fed by large tributaries drive submarine melt rates in excess of 3.0 m d−1 and account for 85% of total estimated melt across the terminus. Nearly the entire terminus is undercut, which may intersect surface crevasses and promote calving. Severe undercutting constricts buoyant outflow plumes and may amplify melt. The observed morphology and melt distribution motivate more realistic treatments of terminus shape and subglacial discharge in submarine melt models

Sigma viruses from three species of Drosophila form a major new clade in the rhabdovirus phylogeny

The sigma virus (DMelSV), which is a natural pathogen of Drosophila melanogaster, is the only Drosophila-specific rhabdovirus that has been described. We have discovered two new rhabdoviruses, D. obscura and D. affinis, which we have named DObsSV and DAffSV, respectively. We sequenced the complete genomes of DObsSV and DMelSV, and the L gene from DAffSV. Combining these data with sequences from a wide range of other rhabdoviruses, we found that the three sigma viruses form a distinct clade which is a sister group to the Dimarhabdovirus supergroup, and the high levels of divergence between these viruses suggest that they deserve to be recognized as a new genus. Furthermore, our analysis produced the most robustly supported phylogeny of the Rhabdoviridae to date, allowing us to reconstruct the major transitions that have occurred during the evolution of the family. Our data suggest that the bias towards research into plants and vertebrates means that much of the diversity of rhabdoviruses has been missed, and rhabdoviruses may be common pathogens of insects

Host-switching by a vertically transmitted rhabdovirus in Drosophila

A diverse range of endosymbionts are found within the cells of animals. As these endosymbionts are normally vertically transmitted, we might expect their evolutionary history to be dominated by host-fidelity and cospeciation with the host. However, studies of bacterial endosymbionts have shown that while this is true for some mutualists, parasites often move horizontally between host lineages over evolutionary timescales. For the first time, to our knowledge, we have investigated whether this is also the case for vertically transmitted viruses. Here, we describe four new sigma viruses, a group of vertically transmitted rhabdoviruses previously known in Drosophila. Using sequence data from these new viruses, and the previously described sigma viruses, we show that they have switched between hosts during their evolutionary history. Our results suggest that sigma virus infections may be short-lived in a given host lineage, so that their long-term persistence relies on rare horizontal transmission events between hosts

Submesoscale processes at shallow salinity fronts in the Bay of Bengal : observations during the winter monsoon

Author Posting. © American Meteorological Society, 2018. This article is posted here by permission of American Meteorological Society for personal use, not for redistribution. The definitive version was published in Journal of Physical Oceanography 48 (2018): 479-509, doi:10.1175/JPO-D-16-0283.1.Lateral submesoscale processes and their influence on vertical stratification at shallow salinity fronts in the central Bay of Bengal during the winter monsoon are explored using high-resolution data from a cruise in November 2013. The observations are from a radiator survey centered at a salinity-controlled density front, embedded in a zone of moderate mesoscale strain (0.15 times the Coriolis parameter) and forced by winds with a downfront orientation. Below a thin mixed layer, often ≤10 m, the analysis shows several dynamical signatures indicative of submesoscale processes: (i) negative Ertel potential vorticity (PV); (ii) low-PV anomalies with O(1–10) km lateral extent, where the vorticity estimated on isopycnals and the isopycnal thickness are tightly coupled, varying in lockstep to yield low PV; (iii) flow conditions susceptible to forced symmetric instability (FSI) or bearing the imprint of earlier FSI events; (iv) negative lateral gradients in the absolute momentum field (inertial instability); and (v) strong contribution from differential sheared advection at O(1) km scales to the growth rate of the depth-averaged stratification. The findings here show one-dimensional vertical processes alone cannot explain the vertical stratification and its lateral variability over O(1–10) km scales at the radiator survey.S. Ramachandran acknowledges support from the National Science Foundation through award OCE 1558849 and the U.S. Office of Naval Research, Grants N00014-13-1-0456 and N00014-17- 1-2355. A. Tandon acknowledges support from the U.S. Office of Naval Research, Grants N00014-13-1-0456 and N00014-17-1-2355. J. T. Farrar and R. A. Weller were supported by the U.S. Office of Naval Research, Grant N00014-13-1-0453, to collect the UCTD data and process theUCTD and shipboard meteorological data. J. Nash, J. Mackinnon, and A. F. Waterhouse acknowledge support from the U. S. Office of Naval Research, Grants N00014-13-1-0503 and N00014-14-1-0455. E. Shroyer acknowledges support from the U. S. Office of Naval Research, Grants N00014-14-10236 and N00014-15- 12634. A. Mahadevan acknowledges support fromthe U. S. Office of Naval Research, Grant N00014-13-10451. A. J. Lucas and R. Pinkel acknowledge support from the U. S. Office of Naval Research, Grant N00014-13-1-0489.2018-08-2

Establishment of Highly Tumorigenic Human Colorectal Cancer Cell Line (CR4) with Properties of Putative Cancer Stem Cells

BACKGROUND: Colorectal cancer (CRC) has the third highest mortality rates among the US population. According to the most recent concept of carcinogenesis, human tumors are organized hierarchically, and the top of it is occupied by malignant stem cells (cancer stem cells, CSCs, or cancer-initiating cells, CICs), which possess unlimited self-renewal and tumor-initiating capacities and high resistance to conventional therapies. To reflect the complexity and diversity of human tumors and to provide clinically and physiologically relevant cancer models, large banks of characterized patient-derived low-passage cell lines, and especially CIC-enriched cell lines, are urgently needed. PRINCIPAL FINDINGS: Here we report the establishment of a novel CIC-enriched, highly tumorigenic and clonogenic colon cancer cell line, CR4, derived from liver metastasis. This stable cell line was established by combining 3D culturing and 2D culturing in stem cell media, subcloning of cells with particular morphology, co-culture with carcinoma associated fibroblasts (CAFs) and serial transplantation to NOD/SCID mice. Using RNA-Seq complete transcriptome profiling of the tumorigenic fraction of the CR4 cells in comparison to the bulk tumor cells, we have identified about 360 differentially expressed transcripts, many of which represent stemness, pluripotency and resistance to treatment. Majority of the established CR4 cells express common markers of stemness, including CD133, CD44, CD166, EpCAM, CD24 and Lgr5. Using immunocytochemical, FACS and western blot analyses, we have shown that a significant ratio of the CR4 cells express key markers of pluripotency markers, including Sox-2, Oct3/4 and c-Myc. Constitutive overactivation of ABC transporters and NF-kB and absence of tumor suppressors p53 and p21 may partially explain exceptional drug resistance of the CR4 cells. CONCLUSIONS: The highly tumorigenic and clonogenic CIC-enriched CR4 cell line may provide an important new tool to support the discovery of novel diagnostic and/or prognostic biomarkers as well as the development of more effective therapeutic strategies

The impact of glacier geometry on meltwater plume structure and submarine melt in Greenland fjords

Meltwater from the Greenland Ice Sheet often drains subglacially into fjords, driving upwelling plumes at glacier termini. Ocean models and observations of submarine termini suggest that plumes enhance melt and undercutting, leading to calving and potential glacier destabilization. Here we systematically evaluate how simulated plume structure and submarine melt during summer months depends on realistic ranges of subglacial discharge, glacier depth, and ocean stratification from 12 Greenland fjords. Our results show that grounding line depth is a strong control on plume-induced submarine melt: deep glaciers produce warm, salty subsurface plumes that undercut termini, and shallow glaciers produce cold, fresh surface-trapped plumes that can overcut termini. Due to sustained upwelling velocities, plumes in cold, shallow fjords can induce equivalent depth-averaged melt rates compared to warm, deep fjords. These results detail a direct ocean-ice feedback that can affect the Greenland Ice Sheet

Mixing to monsoons: Air-sea interactions in the bay of Bengal

More than 1 billion people depend on rainfall from the South Asian monsoon for their livelihoods. Summertime monsoonal precipitation is highly variable on intraseasonal time scales, with alternating "active" and "break" periods. These intraseasonal oscillations in large-scale atmospheric convection and winds are closely tied to 1°C-2°C variations of sea surface temperature in the Bay of Bengal

Distributed subglacial discharge drives significant submarine melt at a Greenland tidewater glacier

Submarine melt can account for substantial mass loss at tidewater glacier termini. However, the processes controlling submarine melt are poorly understood due to limited observations of submarine termini. Here at a tidewater glacier in central West Greenland, we identify subglacial discharge outlets and infer submarine melt across the terminus using direct observations of the submarine terminus face. We find extensive melting associated with small discharge outlets. While the majority of discharge is routed to a single, large channel, outlets not fed by large tributaries drive submarine melt rates in excess of 3.0 m d⁻¹ and account for 85% of total estimated melt across the terminus. Nearly the entire terminus is undercut, which may intersect surface crevasses and promote calving. Severe undercutting constricts buoyant outflow plumes and may amplify melt. The observed morphology and melt distribution motivate more realistic treatments of terminus shape and subglacial discharge in submarine melt models.This is the publisher’s final pdf. The article is copyrighted by American Geophysical Union and published by John Wiley & Sons, Inc. It can be found at: http://agupubs.onlinelibrary.wiley.com/agu/journal/10.1002/%28ISSN%291944-8007

Near-glacier surveying of a subglacial discharge plume: Implications for plume parameterizations

At tidewater glaciers, plume dynamics affect submarine melting, fjord circulation, and the mixing of meltwater. Models often rely on buoyant plume theory to parameterize plumes and submarine melting; however, these parameterizations are largely untested due to a dearth of near‐glacier measurements. Here we present a high‐resolution ocean survey by ship and remotely operated boat near the terminus of Kangerlussuup Sermia in west Greenland. These novel observations reveal the 3‐D structure and transport of a near‐surface plume, originating at a large undercut conduit in the glacier terminus, that is inconsistent with axisymmetric plume theory, the most common representation of plumes in ocean‐glacier models. Instead, the observations suggest a wider upwelling plume—a “truncated” line plume of ∼200 m width—with higher entrainment and plume‐driven melt compared to the typical axisymmetric representation. Our results highlight the importance of a subglacial outlet's geometry in controlling plume dynamics, with implications for parameterizing the exchange flow and submarine melt in glacial fjord models.NNX12AP50