Project Management in State Authorities of Subjects of The Russian Federation: Development Factors and Resources

The article describes the Russian experience in the implementation and development of the project approach in the public administration system at the federal and regional levels. The authors characterize the two-level institutional design of the project management system in the Russian Federation in the form of strategic design and project management. At the same time, the authors note that the greatest variability and riskiness is represented by the project management subsystem, which at the level of the constituent entities of the Russian Federation has a poly-variate character. Based on the results of the empirical study, the authors have identified the factors of an institutional and subjective nature that affect the success of the implementation of project management in the constituent entities of the Russian Federation. The authors also describe a set of resources for the development of a project approach in regional authorities and local self-governments of the Russian Federation: political and administrative, socio-economic, communication-network, personnel

Alternation rate in perceptual bistability is maximal at and symmetric around equi-dominance

When an ambiguous stimulus is viewed for a prolonged time, perception alternates between the different possible interpretations of the stimulus. The alternations seem haphazard, but closer inspection of their dynamics reveals systematic properties in many bistable phenomena. Parametric manipulations result in gradual changes in the fraction of time a given interpretation dominates perception, often over the entire possible range of zero to one. The mean dominance durations of the competing interpretations can also vary over wide ranges (from less than a second to dozens of seconds or more), but finding systematic relations in how they vary has proven difficult. Following the pioneering work of W. J. M. Levelt (1968) in binocular rivalry, previous studies have sought to formulate a relation in terms of the effect of physical parameters of the stimulus, such as image contrast in binocular rivalry. However, the link between external parameters and “stimulus strength” is not as obvious for other bistable phenomena. Here we show that systematic relations readily emerge when the mean dominance durations are examined instead as a function of “percept strength,” as measured by the fraction of dominance time, and provide theoretical rationale for this observation. For three different bistable phenomena, plotting the mean dominance durations of the two percepts against the fraction of dominance time resulted in complementary curves with near-perfect symmetry around equi-dominance (the point where each percept dominates half the time). As a consequence, the alternation rate reaches a maximum at equi-dominance. We next show that the observed behavior arises naturally in simple double-well energy models and in neural competition models with cross-inhibition and input normalization. Finally, we discuss the possibility that bistable perceptual switches reflect a perceptual “exploratory” strategy, akin to foraging behavior, which leads naturally to maximal alternation rate at equi-dominance if perceptual switches come with a cost

Characterization of VPS34-IN1, a selective inhibitor of Vps34, reveals that the phosphatidylinositol 3-phosphate-binding SGK3 protein kinase is a downstream target of class III phosphoinositide 3-kinase

The Vps34 (vacuolar protein sorting 34) class III PI3K (phosphoinositide 3-kinase) phosphorylates PtdIns (phosphatidylinositol) at endosomal membranes to generate PtdIns(3)P that regulates membrane trafficking processes via its ability to recruit a subset of proteins possessing PtdIns(3)P-binding PX (phox homology) and FYVE domains. In the present study, we describe a highly selective and potent inhibitor of Vps34, termed VPS34-IN1, that inhibits Vps34 with 25 nM ICin vitro, but does not significantly inhibit the activity of 340 protein kinases or 25 lipid kinases tested that include all isoforms of class I as well as class II PI3Ks. Administration of VPS34-IN1 to cells induces a rapid dose-dependent dispersal of a specific PtdIns(3)P-binding probe from endosome membranes, within 1 min, without affecting the ability of class I PI3K to regulate Akt. Moreover, we explored whether SGK3 (serum- and glucocorticoid-regulated kinase-3), the only protein kinase known to interact specifically with PtdIns(3)P via its N-terminal PX domain, might be controlled by Vps34. Mutations disrupting PtdIns(3)P binding ablated SGK3 kinase activity by suppressing phosphorylation of the T-loop [PDK1 (phosphoinositide-dependent kinase 1) site] and hydrophobic motif (mammalian target of rapamycin site) residues. VPS34-IN1 induced a rapid ~50-60% loss of SGK3 phosphorylation within 1 min. VPS34-IN1 did not inhibit activity of the SGK2 isoform that does not possess a PtdIns(3)P-binding PX domain. Furthermore, class I PI3K inhibitors (GDC-0941 and BKM120) that do not inhibit Vps34 suppressed SGK3 activity by ~40%. Combining VPS34-IN1 and GDC-0941 reduced SGK3 activity ~80-90%. These data suggest SGK3 phosphorylation and hence activity is controlled by two pools of PtdIns(3)P. The first is produced through phosphorylation of PtdIns by Vps34 at the endosome. The second is due to the conversion of class I PI3K product, PtdIns(3,4,5)P3 into PtdIns(3)P, via the sequential actions of the PtdIns 5-phosphatases [SHIP1/2 (Src homology 2-domain-containing inositol phosphatase 1/2)] and PtdIns 4-phosphatase [INPP4B (inositol polyphosphate 4-phosphatase type II)]. VPS34-IN1 will be a useful probe to delineate physiological roles of the Vps34. Monitoring SGK3 phosphorylation and activity could be employed as a biomarker of Vps34 activity, in an analogous manner by which Akt is used to probe cellular class I PI3K activity. Combining class I (GDC-0941) and class III (VPS34-IN1) PI3K inhibitors could be used as a strategy to better analyse the roles and regulation of the elusive class II PI3K

A self-consistent treatment of non-equilibrium spin torques in magnetic multilayers

It is known that the transfer of spin angular momenta between current carriers and local moments occurs near the interface of magnetic layers when their moments are non-collinear. However, to determine the magnitude of the transfer, one should calculate the spin transport properties far beyond the interface regions. Based on the spin diffusion equation, we present a self-consistent approach to evaluate the spin torque for a number of layered structures. One of the salient features is that the longitudinal and transverse components of spin accumulations are inter-twined from one layer to the next, and thus, the spin torque could be significantly amplified with respect to treatments which concentrate solely on the transport at the interface due to the presence of the much longer longitudinal spin diffusion length. We conclude that bare spin currents do not properly estimate the spin angular momentum transferred between to the magnetic background; the spin transfer that occurs at interfaces should be self-consistently determined by embedding it in our globally diffuse transport calculations.Comment: 21 pages, 6 figure