Additional file 1: Figure S1. of RNA virus interference via CRISPR/Cas13a system in plants

Confirmation of pCas13a expression in planta. Figure S2. pCas13a-mediated interference with TuMV-GFP in planta. Figure S3. GFP quantification of TuMV-GFP interference in transgenic pCas13a-OE plants. (PDF 1393 kb

Additional file 3: Table S1: of RNA virus interference via CRISPR/Cas13a system in plants

TuMV genome description. Table S2: Primers used in this study. (DOCX 21 kb

Additional file 2: Sequence S1: of RNA virus interference via CRISPR/Cas13a system in plants

pCas13a amino acid sequence (3xHA-pCas13a-nls). Sequence S2: pCas13a full-length plant codon optimized DNA sequence (3x-HA-pCas13a-nls) Map S1: pCas13a sequence in pK2GW7-pCas13a (pK2GW7-3xHA-pCas13a-nls). Sequence S3: TuMV-GFP full-length sequence with target sequences in different colors. Map S2: Map of TuMV-GFP. Sequence S4: Cas13a-repeat-cRNA-TuMV-GFP-GFP-target 1 sequence (A), map (B) and its complex with targeting region in TuMV GFP genomic region (C). Sequence S5: Cas13a-repeat-cRNA-TuMV-GFP-GFP-T2 sequence (A), map (B), and its complex with targeting area in TuMV GFP genomic region (C). Sequence S6: Cas13a-repeat-cRNA-TuMV-GFP-HC-Pro-T1 sequence (A), map (B), and its complex with HC-pro targeting region in TuMV (C). Sequence S7: Cas13a-repeat-cRNA-TuMV-GFP-Cp-Pro-T1 sequence (A), map (B), and its complex with Capsid protein targeting region in TuMV. (DOCX 414 kb