Investigating the Association between rs4986790 Polymorphism of TLR4 Gene and Chronic Periodontitis in an Iranian Population: TLR4 Gene Polymorphism and Chronic Periodontitis

Objectives Periodontitis is a multifactorial disease in terms of etiology, including microbial, environmental, systemic, and genetic factors, and the host's immune response causes periodontal destruction. The present study was done to investigate the association between rs4986790 polymorphism of the TLR4 gene with chronic periodontitis in an Iranian population. Method The present case-control study was conducted on 120 people, including two groups of control (n=66) and patient (n=54) in 2022. A periodontist evaluated the periodontal condition of the people, 5 milliliters of blood sample was taken from participants, gene sequence was determined using the PCR-RFLP method, and statistical analysis was performed using the chi-square test to determine the association between the disease and the mentioned polymorphism. Results 64 participants (53.33%) were male, and the rest were female. The frequency of the A allele was 89% in the patient group and 90% in the control group, and the frequency of the G allele was 11% in the patient group and 10% in the control group. Also, the frequency of AA and AG genotypes was 78% and 22% in patients, and 80% and 20% in the control group, respectively (P=0.65). There was no statistically significant association between the genders, the presence of a specific allele or genotype with the occurrence of the disease. The odds ratio (OR) of the developing periodontitis in the heterozygous group compared to the homozygous group was calculated to be 0.78% (P=0.65). Conclusion There was no association between TLR-4 gene polymorphism and chronic periodontitis in the studied population. Keywords TLR4 Receptor; Chronic periodontitis; Single nucleotide polymorphism

Investigating the Relationship between IL-6 Gene (Rs1800795) Polymorphism and Chronic Periodontitis in an Iranian Population: IL-6 Gene Polymorphism and Chronic Periodontitis

Objectives Chronic periodontitis is the most common type of periodontitis and a multifactorial disease in which a set of factors such as microbial plaque, environment, systemic conditions and genetics play a role in its development. Periodontal destruction occurs due to the host's immune response to microbial stimulation, which can lead to tooth loss. The purpose of the present study is to investigate the correlation between IL-6 gene polymorphism (rs1800795) and chronic periodontitis in an Iranian population. Method This case-control analytical study was conducted on 54 patients with chronic periodontitis and 66 healthy individuals referred to the dental clinics of Shahid Beheshti, Zanjan, and Mashhad universities of medical sciences. After clinical examination and classification of subjects, blood samples (10 cc) were taken. The genomic DNA was extracted using salting-out method. The desired variant was amplified using PCR-RFLP method. Reaction products were analyzed after electrophoresis with 1% agarose gel. Data were analyzed with Pearson's chi-square and Hardy-Weinberg equilibrium tests, and P<0.05 was considered as the significant level. Results The frequency of GG, GC, and CC genotypes was 41%, 44%, and 15%, in the patient group, and 36%, 42%, and 21%in the control group, respectively. There was no significant difference between the two groups in terms of the distribution of any of the inheritance patterns (P>0.05). Conclusion The present study showed no relationship between IL-6 gene polymorphism (rs1800795) and chronic periodontitis in the studied population