4 research outputs found
Additional file 3: Figure S2. of microRNA-mediated resistance to hypoglycemia in the HepG2 human hepatoma cell line
No full textc-Myc expression after incubation of cells with various concentrations of glucose. Cells were cultured with 200, 900, and 1800Â mg/L of glucose for 1Â week and the expression of c-Myc protein was examined by Western blotting. The graph under Western blotting shows the result of densitometry of bands. No significant change in this gene expression was detected in different glucose concentrations. (PPTX 89 kb
Additional file 2: Table S1. of microRNA-mediated resistance to hypoglycemia in the HepG2 human hepatoma cell line
No full textChange in the expression of the indicated genes between the hyperglycemic and normoglycemic conditions. Genes whose expression was greatly altered after the incubation of cells with two different concentrations of glucose, 900 mg/dL and 1800 mg/dL, are shown. The 10 genes showing the greatest increase and the 10 genes showing the greatest decrease from 900 mg/L to 1800 mg/L glucose are shown. The difference in the gene expression in HepG2 cells is shown in Table 1a, whereas that of HepaRG cells ® is shown in Table 1b. (DOCX 120 kb
Additional file 1: Figure S1. of microRNA-mediated resistance to hypoglycemia in the HepG2 human hepatoma cell line
No full textDNA microarray analysis after cells were cultured with various concentrations of glucose. HepG2 and HepaRG® cells were cultured with glucose concentrations of 200, 900, and 1800 mg/L for 1 week and the RNA extracted was analyzed by DNA array (3D-Gene®, Toray; http://333.3d-gene.com/ ). Plots of mRNA expressions in HepG2 and HepaRG® cells cultured with 900 mg/L (vertical axis) and 200 mg/L (horizontal axis) glucose (a and c, respectively). MicroRNA expressions in HepG2 and HepaRG® cultured with 1800 mg/L (vertical axis) and 900 mg/L (horizontal axis) glucose are plotted in b and d, respectively. (PPTX 1966 kb
