Evolutionary history of Wolbachia infections in the fire ant Solenopsis invicta

BACKGROUND: Wolbachia are endosymbiotic bacteria that commonly infect numerous arthropods. Despite their broad taxonomic distribution, the transmission patterns of these bacteria within and among host species are not well understood. We sequenced a portion of the wsp gene from the Wolbachia genome infecting 138 individuals from eleven geographically distributed native populations of the fire ant Solenopsis invicta. We then compared these wsp sequence data to patterns of mitochondrial DNA (mtDNA) variation of both infected and uninfected host individuals to infer the transmission patterns of Wolbachia in S. invicta. RESULTS: Three different Wolbachia (wsp) variants occur within S. invicta, all of which are identical to previously described strains in fire ants. A comparison of the distribution of Wolbachia variants within S. invicta to a phylogeny of mtDNA haplotypes suggests S. invicta has acquired Wolbachia infections on at least three independent occasions. One common Wolbachia variant in S. invicta (wSinvictaB) is associated with two divergent mtDNA haplotype clades. Further, within each of these clades, Wolbachia-infected and uninfected individuals possess virtually identical subsets of mtDNA haplotypes, including both putative derived and ancestral mtDNA haplotypes. The same pattern also holds for wSinvictaA, where at least one and as many as three invasions into S. invicta have occurred. These data suggest that the initial invasions of Wolbachia into host ant populations may be relatively ancient and have been followed by multiple secondary losses of Wolbachia in different infected lineages over time. Finally, our data also provide additional insights into the factors responsible for previously reported variation in Wolbachia prevalence among S. invicta populations. CONCLUSION: The history of Wolbachia infections in S. invicta is rather complex and involves multiple invasions or horizontal transmission events of Wolbachia into this species. Although these Wolbachia infections apparently have been present for relatively long time periods, these data clearly indicate that Wolbachia infections frequently have been secondarily lost within different lineages. Importantly, the uncoupled transmission of the Wolbachia and mtDNA genomes suggests that the presumed effects of Wolbachia on mtDNA evolution within S. invicta are less severe than originally predicted. Thus, the common concern that use of mtDNA markers for studying the evolutionary history of insects is confounded by maternally inherited endosymbionts such as Wolbachia may be somewhat unwarranted in the case of S. invicta

Asteroid flux and impact cratering rate on Venus

By the end of 1990, 65 Venus-crossing asteroids were recognized; these represent 59 percent of the known Earth-crossing asteroids. Further studies, chiefly numerical integrations of orbit evolution, may reveal one or two more Venus crossers among the set of discovered asteroids. A Venus crosser was defined as an asteroid whose orbit can intersect the orbit of Venus as a result of secular (long range) perturbations. Venus crossers revolving on orbits that currently overlap the orbit of Venus are called Venapol asteroids, and those on orbit that don't overlap are called Venamor asteroids; 42 Venapols and 23 Venamors were recognized. Collision probabilities with Venus for 60 of the known Venus crossers were determined

Gravity survey of the Mt. Toondina impact structure, South Australia

The Mt. Toondina impact structure is located in northern South Australia, about 45 km south of the town of Oodnadatta. Only the central uplift is exposed. The outcrops at Mt. Toondina reveal a remarkable structural anomaly surrounded by a broad expanse of nearly flat-lying beds of the Bulldog Shale of Early Cretaceous age. A gravity survey was undertaken in 1989 to determine the diameter of the impact structure, define the form of the central uplift, and understand the local crustal structure. Data were collected along two orthogonal lines across the structure. In addition to the profiles, a significant number of measurements were made on and around the central uplift. The 1989 gravity data combined with 1963 gravity data and the seismic reflection data provide an excellent data base to interpret the subsurface structure of the Mt. Toondina feature

Earth-Approaching Asteroids as Targets for Exploration

A mission strategy focused on the earth approaching asteroids is presented. The opportunities for sample return and manned visits favor the closer Apollos and Amors over the main belt objects

Earth-approaching asteroids: Populations, origin, and compositional types

Origin, physical properties, and discovery history of smaller asteroids are reviewed. They appear to link the main belt objects, namely the comets and meteorites. Physical observations suggest that a wide variety of compositional types are represented among the near-earth asteroids; the apparent rarity of carbonaceous objects is stated

Impact cratering and the surface age of Venus: The Pre-Magellan controversy

The average surface age of a planet is a major indicator of the level of its geologic activity and thus of the dynamics of its interior. Radar images obtained by Venera 15/16 from the northern quarter of the Venus (lat 30 to 90 degs) reveal about 150 features that resemble impact craters, and they were so interpreted by Soviet investigators B. A. Ivanov, A. T. Basilevsky, and their colleagues. These features range in diameter from about 10 to 145 km. Their areal density is remarkably similar to the density of impact structures found on the American and European continental shields. The basic difference between the Soviet and American estimates of the average surface age of Venus's northern quarter is due to which crater-production rate is used for the Venusian environment. Cratering rates based on the lunar and terrestrial cratering records, as well as statistical calculations based on observed and predicted Venus-crossing asteroids and comets, have been used in both the Soviet and American calculations. The single largest uncertainty in estimating the actual cratering rates near Venus involves the shielding effect of the atmosphere

Study of flight management requirements during SST low visibility approach and landing operations Final summary report

Flight management operational problems and task requirements for low visibility approach and landing of supersonic transport

Study of flight management requirements during SST low visibility approach and landing operations. Volume 2 - Delineation of potential problems in supporting the performance of flight management tasks

Potential flight management problems during low visibility landing of supersonic transport

K/T boundary stratigraphy: Evidence for multiple impacts and a possible comet stream

A critical set of observations bearing on the K/T boundary events were obtained from several dozen sites in western North America. Thin strata at and adjacent to the K/T boundary are locally preserved in association with coal beds at these sites. The strata were laid down in local shallow basins that were either intermittently flooded or occupied by very shallow ponds. Detailed examination of the stratigraphy at numerous sites led to the recognition of two distinct strata at the boundary. From the time that the two strata were first recognized, E.M. Shoemaker has maintained that they record two impact events. We report some of the evidence that supports this conclusion

Spray Ejected from the Lunar Surface by Meteoroid Impact

Fragments ejected from lunar surface by meteoroid impact analyzed on basis of studies of hypervelocity impact in rock and san