Much Ado About the First Folio at Boise State

In the afternoon of Sat, Aug 20, the Ron and Linda Yanke Family Research Park building was alive with the First Folio in Idaho Grand Opening Carnival

Le Miroir des sports : publication hebdomadaire illustrée

19 février 19351935/02/19 (N812)-1935/02/19

Fraction of CV TRCs labelled with nAChR antibodies.

<p>Fraction of CV TRCs labelled with nAChR antibodies.</p

Immunostaining of α4 nAChR in WT mouse CV and FF taste bud cells.

<p><b>(A)</b> Secondary antibody fluorescence (Alexa Fluor® 488; FITC), <b>(B)</b> DIC image, <b>(C)</b> DAPI, and <b>(D)</b> Merged images of DIC, DAPI and FITC. Panel <b>(P1)</b> shows a low magnification image of a WT mouse CV section. Only a subset of CV taste bud cells demonstrated specific binding to nAChR α4 antibody. Panels <b>(P2)</b> and <b>(P3)</b> show high magnification images of a WT mouse FF taste papillae section. Again, only a subset of FF taste bud cells showed binding to nAChR α4 antibody mainly in the basolateral/intracellular region of TRCs. Panels <b>(P4)</b> and <b>(P5)</b> show that no antibody binding was observed when the primary antibody step was omitted (NC) or in tissue sections from α4 KO mouse (KO), respectively. Horizontal bars = 10 μm.</p

Relative distribution of nAChRs in TRPM5-GFP CV taste bud cells.

<p>Shows the percent distribution of nAChRs in TRPM5-GFP cells in various CV taste papillae sections as a Venn diagram.</p

Detection of qRT-PCR α7 product by gel electrophoresis.

<p>Following qRT-PCR, the final qRT-PCR product was loaded onto 2% agarose gel to detect the presence of the amplified product.</p

Immunostaining of α3 nAChR in WT mouse CV taste bud cells.

<p>The images are overlay of DIC image and secondary antibody fluorescence (Alexa Fluor® 488; FITC). Only a subset of CV taste bud cells showed the binding of nAChR α3 antibody (<b>A</b> and <b>B</b>). No antibody binding was observed when the primary antibody step was omitted (<b>C</b>; NC).</p

Le Miroir des sports : publication hebdomadaire illustrée

31 décembre 19291929/12/31 (A19,N855,SER519)-1929/12/31

Immunostaining of β2 nAChR in WT mouse CV taste bud cells.

<p>The images are merged images of DIC, DAPI, and secondary antibody fluorescence (Alexa Fluor® 488). <b>(A)</b> A low magnification image of mouse CV taste papilla section that shows preferential binding of the nAChR β2 antibody to the apical pole of CV taste bud cells (red arrows). <b>(B)</b> and <b>(C)</b> High resolution images of the CV taste bud cells showing preferential binding of the antibody to the apical pole of the TRCs. Some binding was also observed in the intracellular/basal compartment of TRCs. <b>(D)</b> No antibody binding was observed when the primary antibody step was omitted (NC). Horizontal bars = 10 μm.</p

Immunostaining of α3 nAChR in enteroendocrine cells in the Trpm5-GFP mouse gut.

<p>Shows DIC image, DAPI, Trpm5-GFP, α3 nAChR antibody binding (secondary Donkey/Rabbit-590 antibody), and the merged images of DAPI, GFP and 590 red-fluorescent dye. In each of the 8 slides from 3 different gut sections examined 1 to 4 enteroendocrine cells were positive for α3 nAChR in the crypts (<b>P1, P2, and P3;</b> red). The α3 nAChR-positive cells were also positive for Trpm5-GFP (green). Negative control (NC) without primary antibody <b>(P4)</b>.</p