Phylogenetic analysis.

<p>E1A, fiber and hexon genes, as well as whole genome sequences of HAdV, are analyzed with respect to their phylogenetic relationships. Genes from the three recent HAdV-B14p1 strains are closely related to each other and to the prototype HAdV-B14p genome. It is remarkable that HAdV-B14p1 has a high level of sequence similarity to the prototype genome after approximately 50 years. Phylogenetic analysis was performed using the software MEGA v4.0 (Molecular Genetic Analysis Software; <a href="http://www.megasoftware.net" target="_blank">http://www.megasoftware.net</a>), specifically applying a maximum-composite-likelihood method that generated neighbor-joining and bootstrapped trees of phylogeny with 1,000 replicates; all other parameters were set by default.</p

Genome organization of HAdV-B14p1 coding sequences.

<p>The genome is represented by a central black horizontal line marked at 5-kbp intervals. Protein encoding regions are shown as arrows indicating transcriptional orientation. Forward arrows (above the horizontal black line) show coding regions in the 5’ to 3’ direction and arrows pointing to the left (below the horizontal black line) show the coding regions encoded on the complementary strand. Spliced regions are indicated by a black line joining the coding sequences. Colors are added for contrast between the groups and not indicative of other relationships other than grouping the genes to their transcript, for example, the two red genes of “L1” have no relationships to the eight red genes of “E3”.</p

Percent identities of select HAdV-14p1 strain BJ430 proteins with representative HAdVs from all species and including all species B viruses.

<p>The proteins span the entire genome.</p

Comparison of non-coding sequence motifs between HAdV-B14p1 strains BJ430 and 303600.

<p>Nucleotide signatures and putative functions are indicated, along with locations noted for the genome.</p

Annotation of coding sequences from the genome of HAdV-B14p1 strain BJ430.

<p>Nucleotide locations indicate the start and stop codons, with coding sequences transcribed from the complementary strand designated by “(-)”, e.g., “5596–5608 (-)”. Coding sequences with spliced regions are indicated by double entries in the location column.</p