1 research outputs found
Red and Near-Infrared Light-Directed Cytosolic Delivery of Two Different RNAs Using Photosensitive RNA Carriers
Many
cellular events are thought to be controlled by the temporal
upregulation of multiple RNAs; the timing of the upregulation of these
RNAs is not always the same. In this study, we first show that our
light-directed intracellular RNA delivery method induced high concentrations
of RNA in a short period. This effect was beneficial for the temporal
control of cellular events by functional RNAs. Next, we stimulated
the short-term upregulation of two different RNAs at different time
points. Cytosolic delivery of a first RNA was induced by red light;
thereafter, cytosolic delivery of a second RNA was induced by near-infrared
light. The time difference between the introduction of the first and
second RNA can be short (0.5–4 h) or long (>8 h). This strategy
shows the potential for future applications of the deliberate control
of time-dependent RNA concentration to guide various cellular functions
by multiple RNAs