Revista complutense de educación

Resumen basado en el de la publicaciónSe lleva a cabo una revisión general del procedimiento cloze, procedimiento que es ampliamente conocido y utilizado como instrumento de evaluación de la lectura en los países de habla inglesa pero que apenas es conocido y empleado en España. Dicha revisión hace referencia tanto a los aspectos metodológicos relacionados con dicho procedimiento como a los distintos usos para los que puede emplearse en el campo de la evaluación de la lectura.ES

KSP-positive cells formed tubular structures in Matrigel.

<p>(a) Light microscopy of KSP-positive and KSP-negative cells under the conditions indicated in the upper left corner of the pictures on the day after flow cytometry. A micron scale is shown at the lower right corner of the pictures. (b) Electron microscopy of a cross section of the tubular structures formed by KSP-positive cells. The asterisks indicate the lumens, the arrowheads point to microvilli-like structures, and an arrow indicates a tight junction. Magnification is shown at the lower right corner.</p

Differentiation of KSP-positive cells into each segment of renal tubular cells.

<p>(a) PCR showing the expression of segment-specific genes of renal tubular cells. The samples were KSP-positive cells examined right after cell purification with flow cytometry, KSP-positive cells forming tubular structures co-cultured with NIH3T3-Wnt4, and NIH3T3-Wnt4 alone. The bands were quantified using ImageJ, and graphs normalized against GAPDH were shown. After sorting: a sample of cells collected right after cell sorting of KSP-positive cells. Tubular structures: a sample of KSP-positive cells that form tubular structures and feeder cells of NIH3T3-Wnt4. Feeder alone: a sample of feeder cells, NIH3T3-Wnt4. (b) Immunofluorescence showing Megalin, AQP2 and Podocalyxin in tubular structures formed by KSP-positive cells co-cultured with NIH3T3-Wnt4. A micron scale is shown at the lower right corner.</p

KSP-positive cells were incorporated with renal tubular cells of mouse embryonic kidneys.

<p>KSP-positive and KSP-negative cells were injected into mouse embryonic kidneys. An immunofluorescent analysis was performed on day 3 after the injection. A micron scale is shown at the lower right corner. (a) KSP-positive cells. The arrows indicate the ES cells that incorporated with the renal tubular cells, and a high magnification picture is also shown. (b) KSP-negative cells.</p

Wnt4 enhanced the tubular formation of KSP-positive cells.

<p>(a) Fluorescence microscopy of tubular structures formed by KSP-positive cells derived from CAG-GFP EB3. The upper lane shows pictures of tubular structures of KSP-positive cells co-cultured with NIH3T3-Wnt4, and the lower lane shows those co-cultured with NIH3T3-Mock. Scale bar, 200 µm. (b) Bar graph indicating the numbers of tubular structures under the indicated conditions. One tubular structure from a branching to a branching is counted as one. (c) Immunofluorescence of KSP-positive or KSP-negative cells showing KSP and human-specific mitochondria as a negative control. Scale bar, 400 µm. (d) PCR showing the expression of Osr1, KSP and GAPDH of KSP-positive and -negative cells after three days of 3D culture in Matrigel with NIH3T3-Wnt4.</p

KSP-positive cells derived from mouse ES cells showed the characteristics of developing kidneys.

<p>(a) Flow cytometry for KSP in CAG-GFP EB3 on day 18 of the differentiation. Cells were gated using side scatter and forward scatter, and dead cells were excluded using propidium iodide. Negative control: Cells incubated with no antibody. 2nd antibody: Cells incubated with only streptavidin-Alexa Fluor 647. 1st/2nd antibody: Cells incubated with anti-KSP antibody and streptavidin-Alexa Fluor 647. (b) Heat map of kidney development (GO:0001822) for KSP-positive and KSP-negative cells derived from CAG-GFP EB3. The microarray data was deposited in the Gene Expression Omnibus (GSE40694, <a href="http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE40694" target="_blank">http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE40694</a>).</p

HGF and IGF-1 enhanced the expression of KSP combined with Activin.

<p>(a) Induction method for evaluating the effects of HGF and IGF-1 on mouse ES cells. (b) Expression of KSP in conditions with HGF or IGF-1 determined using real-time PCR at day 18. The values were normalized against GAPDH. The asterisk indicates <i>P</i><0.05 versus Activin (10 ng/mL) alone. (c) Flow cytometry for KSP in CAG-GFP EB3 at day 18 of the differentiation with Activin and IGF-1.</p

Additional file 2: of Yields and chondrogenic potential of primary synovial mesenchymal stem cells are comparable between rheumatoid arthritis and osteoarthritis patients

Representative raw data for surface markers. Flow cytometric analyses of digested cells before plating (day 0) and expanded cells cultured for 14 days. Donor number of RA and OA patients shown. (DOCX 16 kb