Ploidy analysis and phenotypic characterization of autopolyploid.

<p>A: Ploidy analysis of 2<i>x</i> (A1), 3<i>x</i> (A2), 4<i>x</i> (A3) woad by flow cytometer. B: Mitotic metaphase of 4<i>x</i> plant with 28 chromosomes (B1) and 2<i>x</i> plant with 14 chromosomes (B2). C-G: Morphological differences between diploid and autotetraploid, C-G: plantlets, inflorescence, flowers, pollen grains and siliques of autotetraploid (left) and diploid (right). Scale bars: B, F = 10 μm; C = 10 cm; D, E, G = 1 cm.</p

Analysis of differently expressed genes via pairwise contrasts of different ploidy with | log2Ratio | ≥ 1.0 and FDR ≤ 0.001.

<p>Analysis of differently expressed genes via pairwise contrasts of different ploidy with | log2Ratio | ≥ 1.0 and FDR ≤ 0.001.</p

Unigenes annotatation and characteristics of homology search of unigenes against the nr database.

<p>A: Venn diagram of number of unigenes annotated by BLASTx with an E-value threshold of 10^-5 against the 5 databases. B: E-value distribution of the top BLAST hits against the nr database for each unique sequence. C: Similarity distribution of the top BLAST hits against the nr database for each unique sequence. D: Species distribution of unigenes in the nr database.</p

Functional classification of some differentially expressed genes (DEGs) in diploid vs tetraploid.

<p>Functional classification of some differentially expressed genes (DEGs) in diploid vs tetraploid.</p

Photosynthetic rate of woad with different ploidy.

<p>A, B, C revealed that the photosynthetic rate were significantly different (P<0.01) among 2<i>x</i>, 3<i>x</i> and 4<i>x</i> plants.</p

Classification of GO annotations.

<p>The x-axis indicates the sub-categories; the left y-axis indicates the percentage of a sub-category of genes in that category and the right y-axis indicates the number of unigenes in a sub-category.</p