Metformin effects on EMT markers in two glucose levels.

<p>(A) The cells were treated with metformin for 72 hours in two glucose concentrations and the desired genes were quantified by real-time qPCR. For normalization, GAPDH was amplified in each sample. Each column shows the mean and SD of three independent experiments, performed in triplicate. (B) Western blot analysis of cells treated with 10 mM metformin for 24 to 72 hours in two different glucose concentrations. The GAPDH band, which is considered as control, confirms the integrity and equal loading of protein.</p

Glucose effects on metformin-inhibited cell invasion.

<p><b>(</b>A & B) AGS cells were treated with metformin for 24 hours and the cell invasion was measured in the Culturex 96 well BME invasion assay kit by counting the number of cells invading underside of BME (*<i>p</i> ≤ 0.01, **<i>p</i> ≤ 0.001 and ***<i>p</i> ≤ 0.0001). (C) Effect of high- and low-glucose media on cell invasion analyzed by JMP and levels not connected by same letter are significantly different (<i>p</i> ≤ 0.05).</p