A comprehensive review of anticancer mechanisms of action of Alantolactone

Cancer is considered as the main challenge of human communities, and it annually imposes a significant economic burden on society. Natural products have been used for treatment of many diseases including inflammation, infections, neurological disorders, atherosclerosis, asthma and cancer for many years. Sesquiterpene lactones (STLs) refers to a group of natural products with different biological activities. A type of STL that has recently attracted much attention is Alantolactone (ALT). In recent years, many studies have investigated the molecular mechanism of this compound affecting cancer cells and results suggest that this compound exerts its anticancer effects by providing free radicals and inhibiting some of the signaling pathways that are effective in progression of cancer cells. The present study is aimed to introduce the latest molecular mechanisms of ALT proposed by researchers in recent years

Relative expression of Oct4 and NANOG at early and late passages.

<p>Expression level of Oct4 and NANOG were high at P5 (early passage) compared with P7 (late passage) (P< 0.05).</p

Isolation, Characterization, Cryopreservation of Human Amniotic Stem Cells and Differentiation to Osteogenic and Adipogenic Cells - Fig 4

<p>A and D) total population of cells are presented and highlighted population are transferred and tested for different markers, B) CD90-EP positive cells, C) CD44-FITC positive cells, and E) CD45 negative cells and F) C31 negative cells population.</p

Primers used for RT-PCR.

<p>Primers used for RT-PCR.</p

Regulators which can regulate both NANOG, Oct-4, adipogenesis and osteogenesis.

<p>The network was constructed based on Common Regulators algorithm and the relationships were extracted by text mining using Pathway Studio 11.0.5 (Elsevier).</p

The summarized Common Targets network, keeping only entities which have interactions with POUSF1 and NANOG as well as ossification, and osteogenesis.

<p>The summarized Common Targets network, keeping only entities which have interactions with POUSF1 and NANOG as well as ossification, and osteogenesis.</p

The cellular location of the summarized Common Targets network, keeping only entities which have interactions with POUSF1 and NANOG as well as ossification, and osteogenesis.

<p>The cellular location of the summarized Common Targets network, keeping only entities which have interactions with POUSF1 and NANOG as well as ossification, and osteogenesis.</p

The growth curve of HAFCs in different passages.

<p>As shown here, by increasing the time of cell culture, the cell number increased. The growth rate of P4 is faster than P7 and P10. Except the p-value of 0 h vs 24 h, other p-vales were significant.</p