1 research outputs found
Platinum(II)-Oligonucleotide Coordination Based Aptasensor for Simple and Selective Detection of Platinum Compounds
Wide
use of platinum-based chemotherapeutic regimens for the treatment
for carcinoma calls for a simple and selective detection of platinum
compound in biological samples. On the basis of the platinumÂ(II)-base
pair coordination, a novel type of aptameric platform for platinum
detection has been introduced. This chemiluminescence (CL) aptasensor
consists of a designed streptavidin (SA) aptamer sequence in which
several base pairs were replaced by G-G mismatches. Only in the presence
of platinum, coordination occurs between the platinum and G-G base
pairs as opposed to the hydrogen-bonded G-C base pairs, which leads
to SA aptamer sequence activation, resulting in their binding to SA
coated magnetic beads. These Pt-DNA coordination events were monitored
by a simple and direct luminol-peroxide CL reaction through horseradish
peroxidase (HRP) catalysis with a strong chemiluminescence emission.
The validated ranges of quantification were 0.12–240 μM
with a limit of detection of 60 nM and selectivity over other metal
ions. This assay was also successfully used in urine sample determination.
It will be a promising candidate for the detection of platinum in
biomedical and environmental samples