RUNX3 Mediates Suppression of Tumor Growth and Metastasis of Human CCRCC by Regulating Cyclin Related Proteins and TIMP-1

Here we presented that the expression of RUNX3 was significantly decreased in 75 cases of clear cell renal cell carcinoma (CCRCC) tissues (p<0.05). Enforced RUNX3 expression mediated 786-O cells to exhibit inhibition of growth, G1 cell-cycle arrest and metastasis in vitro, and to lost tumorigenicity in nude mouse model in vivo. RUNX3-induced growth suppression was found partially to regulate various proteins, including inhibition of cyclinD1, cyclinE, cdk2, cdk4 and p-Rb, but increase of p27Kip1, Rb and TIMP-1. Therefore, RUNX3 had the function of inhibiting the proliferative and metastatic abilities of CCRCC cells by regulating cyclins and TIMP1

MiR-218 Inhibits Invasion and Metastasis of Gastric Cancer by Targeting the Robo1 Receptor

MicroRNAs play key roles in tumor metastasis. Here, we describe the regulation and function of miR-218 in gastric cancer (GC) metastasis. miR-218 expression is decreased along with the expression of one of its host genes, Slit3 in metastatic GC. However, Robo1, one of several Slit receptors, is negatively regulated by miR-218, thus establishing a negative feedback loop. Decreased miR-218 levels eliminate Robo1 repression, which activates the Slit-Robo1 pathway through the interaction between Robo1 and Slit2, thus triggering tumor metastasis. The restoration of miR-218 suppresses Robo1 expression and inhibits tumor cell invasion and metastasis in vitro and in vivo. Taken together, our results describe a Slit-miR-218-Robo1 regulatory circuit whose disruption may contribute to GC metastasis. Targeting miR-218 may provide a strategy for blocking tumor metastasis

MAGNETIC PROPERTIES I : ELECTRONIC T2 AND T1 IN UNDOPED (CH)x AND (CD)x

Nous avons mesuré la grandeur et la dépendance de la température de T2e et T1e dans le polyacétylène pur. On donne un résumé des mesures de T2e et aussi des valeurs des vitesses de diffusion des solitons qui sont déduites de ces résultats. On présente quelques mesures de T1e de (CD)x et (CH)x à 9,5 GHz et 16,7 GHz. Ces résultats sont utilisés pour calculer les temps de relaxation nucléaire, T1p, par le mécanisme de la diffusion des spins nucléaires aux impuretés paramagnétiques immobiles. Nous démontrons que les résultats de ces calculs sont en accord avec les mesures par RMN certainement aussi bien que le modèle de diffusion des solitons.We have measured the magnitude and temperature dependences of T2e and T1e in undoped polyacetylene. The T2e measurements and the determination of soliton diffusion rates from them are summarised. New T1e measurements on (CD)x and (CH)x at 9.5 GHz and 16.7 GHz are presented. These measurements are utilised to compute nuclear spin relaxation times, T1p, via the mechanism of nuclear spin diffusion to fixed paramagnetic impurities. It is shown that the results of this calculation agree with published NMR measurements at least as well as does the soliton diffusion model

MAGNETIC PROPERTIES II : ENDOR OF UNDOPED CIS-(CH)x

Nous avons étudié la spectroscopie de double résonance électronique et nucléaire (ENDOR) du cis-polyacétylène enrichi à 99% en 13C à des températures entre 77 K et 300 K. Les spectres sont caractérisés par deux, et deux seulement, tenseurs hyperfins du 1H et du 13C. Les amplitudes relatives et la symétrie des éléments du tenseur établissent que l'électron paramagnétique correspond à une orbitale délocalisée. Les résultats expérimentaux indiquent que l'électron délocalisé se déplace dans un puits de potentiel de ~ 100 Å de largeur.Pristine 99% 13C-enriched cis-polyacetylene has been investigated by electron-nuclear double-resonance (ENDOR) spectroscopy at temperatures between 77 and 300 K. Spectra are characterized by two and only two 1H and 13C hyperfine tensors. The relative magnitudes and symmetry of the tensor elements establish that the paramagnetic electron resides in a delocalized orbital. Experimental results indicate that the delocalized electron is constrained to a potential well of approximately 100 Å in width

Self-assembly of periodic nanoclusters of Si and Ge along atomically straight steps of a vicinal Si(111)

The very initial stage of the molecular beam epitaxy of Si and Ge on Si(111)-7x7 substrates with atomically straight steps has been studied by scanning tunneling microscopy and spectroscopy. The atomically straight steps have been prepared on a miscut Si(111) substrate by annealing at 830 degrees C with kink-up direct current. The length of the steps can be maximized by selecting a proper annealing time. The steps have a well-defined U(2, 0) step-edge structure. The growth of both Si and Ge at temperatures between 250 and 400 degrees C starts with formation of a single-adatom-row nanowire (0.67 nm in width) along the lower edge of each U(2, 0) step. Subsequent growth of Si and Ge at temperatures between 250 and 300 degrees C results in formation of one-dimensional arrays of nanoclusters (less than 2.0 nm in width) in the unfaulted halves of the 7x7 structure along the upper step edges. Scanning tunneling spectroscopy reveals localized electronic states of the nanoclusters. Differences between the growth of Si and Ge nanoclusters are discussed