1 research outputs found
Cytotoxicity Evaluation of Iranian Propolis and Calcium Hydroxide on Dental Pulp Fibroblasts
Abstract
Background and aims. Since intracanal medicaments can affect the cell viability in periapical tissues, the aim of this
study was to evaluate the effect of calcium hydroxide and propolis on pulp fibroblasts.
Materials and methods. Two healthy third molars were used as a source to obtain fibroblasts. The fibroblasts were
cultured and subjected to 1 mg/mL of propolis and calcium hydroxide. This experiment was performed in six replicates and
cell viability was evaluated with MTT assay. Statistical analysis was performed by t-test.
Results. Comparison of cell viability with the use of 1 mg/mL of calcium hydroxide and propolis showed that cells subjected
to propolis were more viable when compared to calcium hydroxide (P < 0.05).
Conclusion. In this study, calcium hydroxide reduced fibroblast viability, significantly more than Iranian propolis. Other
properties should be evaluated before Iranian propolis could be indicated for use as intracanal medicament