Health management at the regional level: multivariable performance assessmen

This article provides an analysis of areas for improving the state policy bases in public health services. The purpose of the research is to substantiate and develop systematized regulatory support for the structural-functional care model for children in particular regions. The authors systematized the legislative acts on the health technologies introduction in the structural-functional mode

Alkyl Sulfatase of Cholera Vibrios

The aim of the work was to study the structure of the alkyl sulfatase (asu) gene in Vibrio cholerae strains of various serogroups, as well as to compare nucleotide and amino acid sequences of alkyl sulfatases using various methods of bioinformatic analysis.Materials and methods. 483 strains of V. cholerae O1, O139 and nonO1/nonO139 serogroups were employed in the work. The search for the gene, its recurrence, and localization was carried out applying the Blast software. The nucleotide and corresponding amino acid sequences of the gene, as well as its structure, were studied using bioinformatic analysis. Sequencing was performed on the MiSeq (Illumina) platform. The enzymatic activity was detected using a medium, confirming the presence/absence of the gene by PCR in vitro and in silico.Results and discussion. Bioinformatic analysis of the nucleotide and corresponding amino acid sequences of the asu gene has been carried out and its structure investigated. Four functional domains have been identified. In the beta-lactamase domain, a conservative amino acid sequence -HAHADH- has been found in all strains of cholera vibrios, which is part of the Zn2+ binding motif. It has been established that the alkyl sulfatase of cholera vibrios belongs to the family of Zn2+-dependent β-lactamases. Blast analysis has revealed the similarity of nucleotide and amino acid sequences of alkyl sulfatases in representatives of V. cholerae O1 and O139 serogroups (ctxAB+tcpA+) and representatives of the genera Aeromonas and Pseudomonas, which is in the line with the data of 3D modeling of the amino acid sequence structures of the alkyl sulfatase enzyme in these microorganisms. The bioinformatic analysis of nucleotide and amino acid sequences of alkyl sulfatases in cholera vibrios has showed the conservativeness of these sequences in toxigenic strains and the presence of a number of single mutations in the asu gene in atoxigenic ones. The presence or absence of the asu gene has been established by PCR in vitro and in silico and confirmed by the results obtained using the Blast program. It is demonstrated that the presence/absence of the asu gene correlates with the ability/inability of O139 strains to hydrolyze SDS on the medium. These results can be used in studying mechanisms of cholera vibrios adaptation, persistence and pathogenicity

ESTIMATED DETERMINATION OF PARAMETERS OF MOUNTING MASTS

When carrying out installation work at the enterprises of the branch, the use of universal lifting equipment is sometimes impossible or impractical. In such cases, resort to the use of mounting masts - relatively simple in design and quite efficient lifting equipment [1], the construction of which is largely based on the practical experience of foremen of assembly organizations or fore-men-riggers. Existing methods of calculation of mounting masts, developed many decades ago on the basis of sufficiently primitive considerations, without taking into account additional loads, give approximate results and should be revised and improved. An attempt at such work is reflected in this article. Since the main structural elements of the mounting mast are its riser and cables, the definition of the parameters of the mast is to calculate the strength of the riser and cables of the accepted dimensions under the loads provided by the initial data of a specific technical task. Evaluation of the strength of the riser should be performed both in the plane of torque and from the plane of torque. To find the value of the maximum stress in the mast riser and the maximum stress in the i-th cable, it is necessary to calculate the stress-strain state of the riser and cables using a mixed method, according to which some unknowns are displacement and part - effort. The mathematical model of the problem is described by four algebraic equations. The equation of continuity of riser deformations is based on the use of Tymoshenko's functions, which take into account the influence of longitudinal forces on the deflection of the mast riser in the plane of action of wind load and weight of cargo. Loads from the weight and tension of the cables are taken into account their possible ice and the force of pre-tension. The wind load of the riser and cables is determined using data on the standard speed pressure, N / m2, which for Ukraine is 0.38 kPa. Calculations of riser and cable parameters also take into account the temperature difference, which in the climatic conditions of Ukraine does not exceed the interval 040 st structures are recommended to rely on the combination of both permanent and temporary loads according to the data given in the article. All this together gives reason to hope that the considered method of calculation of parameters of assembly masts will be useful to experts of the assembly organizations and students studying questions of technics and technology of rigging works

SCIENTIFIC SUPPORT OF FOUNDRY-METALLURGICAL AND OTHER POWER-CONSUMING INDUSTRIES. RESULTS AND PERSPECTIVES

The domestic developments carried out for the last three years in the field of foundry-metallurgical, thermal, galvanic productions are described in detail

SCIENTIFIC SUPPORT OF METALLURGICAL AND OTHER POWER-INTENSIVE BRANCHES IS ONE OF THE MOST IMPORTANT TASKS OF SCIENCE OF THE COUNTRY

Investigations and developments in the field of creation of equipment and technologies of foundry-metallurgical production are given

HISTORY AND DEVELOPMENT DIRECTIONS OF INDUCTION HEATING HFC AT THE MINSK AUTOMOBILE PLANT

The data on the development of induction technology in the Republic of Belarus on the example of the Minsk Automobile Plant are given. Their development began at the end of the fifties at the Minsk Tractor Plant, and the highest development has been obtained at the Minsk Automobile Plant from 1983 to 2008. The short features of energy-efficient processes created at MAZ in collaboration with country’s institutions are given: surface hardening on the contour of gear wheels, a body-surface-hardened gears and satellites, the impulse hardening of complex configuration, the heat treatment of long items and bulk materials, etc.Литератур

PROGRAM “METALLURGY” IS IMPORTANT ELEMENT OF SCIENTIFIC SUPPORT OF PROGRAM OF TECHNICAL RETOOLING OF ENERGY-INTENSIVE PRODUCTIONS OF THE REPUBLIC OF BELARUS

The main directions and developments of Program “Metallurgy” for years 2007–2010 are given

PROGRAM «METALLURGY» - RESULTS AND PROSPECTS

The main results of carying out of state program of applied scientific research «Metallurgy» both in the field of theoretical and practical developments are given

INFLUENCE OF THE THERMAL PROCESS STAGE ON THE SIZE OF VALID GRAIN OF CONSTRUCTIONAL CARBURIZED STEEL GRADE 20CHN3A

Results of studies of the kinetics of changes in actual grains during technological process at different stages of the manufacture of parts with the use of a universal metallographic etchant to identify borders of valid grains in rolling and stamping in carburizing layers and core parts made of alloy structure steel grades are presented

TECHNIQUE OF QUALITY CONTROL OF STRUCTURE OF TOOTH GEARS OF THE MOTOR AND TRACTOR MACHINERY TRANSMISSIONS

The reactant for microstructure quality assurance of cemented layer and a core of transmissions gears of the motor and tractor technique, enabling to reveal and identify the structural components influencing working capacity of details, is offered