Phosphorus and calcium balance and net absorption for control and HP diets.

<p>Data represent means ± SE (<i>n</i> = 5).</p><p>*<i>p</i><0.05 compared to the control group.</p><p>Net absorption and retention were calculated as follows:</p><p>Net absorption (mg/day) = intake – fecal excretion.</p><p>Retention (mg/day) = net absorption – urinary excretion.</p

GO terms that were significantly enriched (<i>p</i><0.05) in the top 1056 genes upregulated in response to a HP diet.

<p>*GO term with no <i>p</i>-value means not significant.</p><p>**FDR-corrected <i>p</i>-values of the GO terms appearing in the deepest hierarchy are represented by bold style.</p

Expression of NaPi-IIb in the kidney in control and HP rats.

<p>Kidney sections from control (A, B) and HP (C, D) rats were analyzed by <i>in situ</i> hybridization using a NaPi-IIb-specific probe. Sections represent the cortex (A, C) and medulla (B, D). Scale bar = 200 µm.</p

Genes related to water, phosphorus and calcium transport whose expression was altered in response to a HP diet.

<p>Genes related to water, phosphorus and calcium transport whose expression was altered in response to a HP diet.</p

Histological analysis of kidney tissue sections from rats fed a control or HP diet.

<p>Kidney sections from control rats (A, B, C) and HP rats (D, E, F) were stained with H&E and visualized by microscopy. Sections correspond to cortex (A, D), the corticomedullary junction (B, E) and the medullary region (C, F). Arrowheads indicate representative cyst-like areas; dotted circles indicate representative fibrosis-like areas. Scale bar = 100 µm.</p

GO terms that were significantly enriched (<i>p</i><0.05) in the top 712 genes downregulated in response to a HP diet.

<p>*GO term with no <i>p</i>-value means not significant.</p><p>**FDR-corrected <i>p</i>-values of the GO terms appearing in the deepest hierarchy are represented by bold style.</p

Composition of the experimental diets.

<p>Composition of the experimental diets.</p

Body weight, weight gain and food intake in control and HP rats.

<p>Data represent means ± SE (<i>n</i> = 5).</p

Effect of a HP diet on fractional excretion of calcium and phosphate in the kidney.

<p>Data represent means ± SE (<i>n</i> = 6 or 7). *<i>p</i><0.05 compared to the control group.</p

GO terms associated with the genes that were down-regulated in the iron-deficient group.

<p>FDR-corrected <i>P</i>-values were defined by the modified Fisher's exact test with the Benjamini and Hochberg FDR correction. FDR-corrected <i>P</i>-values<0.05 are shaded in gray.</p