Expression analysis of <i>S-ELF3</i> transcripts.

<p>cDNA prepared from roots, stems, leaves, pistils, stamens and pollen was used for RT-PCR analysis. <i>ACTIN</i> was amplified as a loading control. DBF: one day before flowering. DF: the day of flowering.</p

Expression of genes selected by in <i>silico</i> subtraction as determined by RT-PCR.

<p>The expression of genes corresponding to the 15 contigs selected by <i>in silico</i> subtraction was examined by RT-PCR using cDNA from long styles (LS) and short styles (SS) as templates. See <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0031264#pone.0031264.s007" target="_blank">Table S1</a> for RT-PCR primers.</p

PCR survey of <i>S-ELF3</i> (<i>SSG3</i>) in 47 buckwheat landraces and modern cultivars.

<p>The numbering of individual plants corresponds to that shown in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0031264#pone.0031264.s008" target="_blank">Table S2</a>. L, long-styled plant. S, short-styled plant. N, negative control. M, 1-kb DNA ladder (GenDireX).</p

Dimorphic flowers of buckwheat and schematic presentation of the intra-morph incompatibility response in buckwheat.

<p>Short-styled flowers of buckwheat have long stamens and vice versa. A pollen grain from a long-styled plant germinates and the pollen tube successfully elongates to reach the ovary in the pistil of a short-styled plant, whereas it germinates but fails to elongate in the style of long-styled flower.</p

<i>S-ELF3</i> in <i>Fagopyrum</i> species.

<p>The gene structure and phylogeny of <i>S-ELF3</i> in five <i>Fagopyrum</i> species, including the SC Kyushu PL4 line, which contains the <i>S^h</i> allele of <i>F. homotropicum</i>, are shown. Species in blue and red font exhibit heteromorphic SI and homomorphic SC, respectively. Dark brown boxes and lines represent 5′- and 3′-untranslated regions and introns, respectively. Coding regions are colored blue. Red boxes and line indicate large insertions (>400 bp) and nonsense mutation, respectively. The phylogenetic tree in the inset was obtained by the Neighbor-joining method. The <i>S-ELF3</i> sequence from <i>F. urophyllum</i> was used as an outgroup. The bootstrap numbers (500 replicates) are shown next to the branches. The scale bar corresponds to 0.02 substitution per nucleotide site.</p

Quantitative analysis of ossification by micro-computed tomography (CT).

<p>A, Micro-computed tomography of the cervical spine of <i>Enpp1^ttw/ttw</i> mice at 12 weeks of age with or without Runx2 haploinsufficiency. 3D reconstructed images.Ectopically calcified region is shown in yellow. B, Quantitative analysis of the ossification of the cruciform ligament at the atlanto-occipital area using micro-CT in <i>Enpp1^ttw/ttw</i> mice at 8 weeks of age with or without Runx2 haploinsufficiency. Note a significant decrease in calcified region in <i>ENPP1^ttw/ttw</i>/<i>Runx2^+/−</i> mice. C,D Micro-CT analysis of the ossification of the cruciform ligament at the atlanto-occipital area in <i>Enpp1^ttw/ttw</i> mice at 12 weeks of age with or without Runx2 haploinsufficiency. Bone mineral content (C) Bone mineral density (D).</p

Runx2 haploinsufficiency ameliorates the development of OPLL.

<p>A–C, Histological (A and B) and immunohistochemical (C) analyses of the cruciform ligament at the atlanto-occipital area in <i>Enpp1^ttw/ttw</i> mice at 8 weeks (A and B) or 4 weeks (C) of age with (right) or without (left) Runx2 haploinsufficiency. (A: H&E staining; B: von Kossa staining.) Note a decrease in calcified region (B) and Runx2 immunoreactivity (C) in <i>ENPP1^ttw/ttw</i>/<i>Runx2^+/−</i> mice. Bottom panels are higher magnification images.(A and C).</p

GenBank sequences used for Figure 1.

<p>GenBank sequences used for <a href="http://www.plosntds.org/article/info:doi/10.1371/journal.pntd.0001843#pntd-0001843-g001" target="_blank">Figure 1</a>.</p

Similarity between dog strain F059 and closest <i>Bartonella</i> species for <i>glt</i>A, ITS and <i>rpo</i>B^*.

*<p>Percentage and GenBank accession numbers.</p

Univariate analysis^* of factors associated with <i>Bartonella</i> PCR detection in Iraqi canids.

*<p>Via logistic regression, with proportion PCR positive, odds ratios, 95% confidence intervals, and chi-square p-values.</p