研究者養成型大学院カリキュラムの日米比較研究

平成15～17年度科学研究費補助金基盤研究(C) 報告書課題番号:155304

N-(Fluoren-9-ylmethoxy­carbon­yl)-l-aspartic acid 4-tert-butyl ester

The bond distances and bond angles of the title compound, C23H25NO6, are consistent with values typically found for fluoren-9-ylmethoxy­carbonyl-protected amino acids. The conformations of the backbone and the side chain are slightly different from those of l-aspartic acid. The crystal structure exhibits two inter­molecular hydrogen bonds, forming a two-dimensional sheet structure parallel to the ab plane

A novel approach to the synthesis of oligodeoxyribonucleotide boranophosphates

Deoxyribonucleoside 3′-boranophosphate derivatives including adenine, cytosine, guanine, and thymine bases were synthesized in good yields by the use of a new boranophosphorylation reaction. The reaction was found to be effective for the formation of internucleotidic boranophosphate linkages

N-(Fluoren-9-ylmethoxy­carbon­yl)-l-leucine

The title compound [systematic name: fluoren-9-yl N-(1-carb­oxy-3-methyl­butyl)carbamate], C21H23NO4, exhibits torsion angles that vary from the typical values found in other Fmoc-protected amino acids, viz. the orientations of the fluorene and carboxyl groups [C—O—C—C = 93.8 (2) and N—C—C=O = −23.6 (2)°]. The crystal structure exhibits two inter­molecular hydrogen bonds (O—H⋯O and N—H⋯O) that link the mol­ecules into two-dimensional sheets parallel to the ab plane

Partial liquid ventilation does not affect BALF TNF-, MIP-2, CINC-1 concentrations, or CD11b cell surface expression, but does increase macrophage proportion among BALF cells in the acute phase of rat LPS-induced lung injury.

To elucidate the mechanism of anti-inflammatory effect of partial liquid ventilation (PLV), cytokine concentration, surface CD11b, and macrophage expression were investigated in BALF. The 30-minutes group was treated with gas ventilation (GV) for 30 minutes after intratracheal LPS administration. The GV group was prepared in the same manner as the 30-minutes group, then the GV was continued for the following 2 hours. The PLV group was treated in the same manner as the 30-minutes group, and then received PLV with perflubron for the following 2 hours. Animals were euthanized to receive BAL. The PLV group showed a tendency to have a higher concentration than the GV group of TNF-alpha, MIP-2, and CINC-1 as measured by ELISA, although there were no significant differences. The ratio of expressions of CD11b and macrophages to total leukocytes were determined by flow-cytometry. There were no significant differences in the ratio of CD11b-positive expression to acquired cells (GV: 63.6 +/- 8.4%, PLV: 60.5+/-5.4%, P=0.73). However, the proportion of macrophages was significantly increased (GV: 5.6 +/-1.5, PLV: 14.0+/-1.3, P=0.006). These results suggest that the anti-inflammatory effect of PLV is not caused by the change in CD11b expression, and that PLV affects the proportion of macrophage among BALF cells.</p