Hyperglycaemia Stress-Induced Renal Injury is Caused by Extensive Mitochondrial Fragmentation, Attenuated MKP1 Signalling, and Activated JNK-CaMKII-Fis1 Biological Axis

Background/Aims: Hyperglycaemia stress-induced renal injury is closely associated with mitochondrial dysfunction through poorly understood mechanisms. The aim of our study is to explore the upstream trigger and the downstream effector driving diabetic nephropathy via modulating mitochondrial homeostasis. Methods: A diabetic nephropathy model was generated in wild-type (WT) mice and MAP Kinase phosphatase 1 transgenic (MKP1-TG) mice using STZ injection. Cell experiments were conducted via high-glucose treatment in the human renal mesangial cell line (HRMC). MKP1 overexpression assay was carried out via adenovirus transfection. Renal function was evaluated via ELISA, western blotting, histopathological staining, and immunofluorescence. Mitochondrial function was determined via mitochondrial potential analysis, ROS detection, ATP measurement, mitochondrial permeability transition pore (mPTP) opening evaluation, and immunofluorescence for mitochondrial pro-apoptotic factors. Loss- and gain-of-function assays for mitochondrial fragmentation were performed using a pharmacological agonist and blocker. Western blotting and the pathway blocker were used to establish the signalling pathway in response to MKP1 overexpression in the presence of hyperglycaemia stress. Results: MKP1 was downregulated in the presence of chronic high-glucose stress in vivo and in vitro. However, MKP1 overexpression improved the metabolic parameters, enhanced glucose control, sustained renal function, attenuated kidney oxidative stress, inhibited the renal inflammation response, alleviated HRMC apoptosis, and repressed tubulointerstitial fibrosis. Molecular investigation found that MKP1 overexpression enhanced the resistance of HRMC to the hyperglycaemic injury by abolishing mitochondrial fragmentation. Hyperglycaemia-triggered mitochondrial fragmentation promoted mitochondrial dysfunction, as evidenced by decreased mitochondrial potential, elevated mitochondrial ROS production, increased pro-apoptotic factor leakage, augmented mPTP opening and activated caspase-9 apoptotic pathway. Interestingly, MKP1 overexpression strongly abrogated mitochondrial fragmentation and sustained mitochondrial homeostasis via inhibiting the JNK-CaMKII-Fis1 pathway. After re-activation of the JNK-CaMKII-Fis1 pathway, the beneficial effects of MKP1 overexpression on mitochondrial protection disappeared. Conclusion: Taken together, our data identified the protective role played by MKP1 in regulating diabetic renal injury via repressing mitochondrial fragmentation and inactivating the JNK-CaMKII-Fis1 pathway, which may pave the road to new therapeutic modalities for the treatment of diabetic nephropathy

Revisiting the concentration observations and source apportionment of atmospheric ammonia

While China’s Air Pollution Prevention and Control Action Plan on particulate matter since 2013 has reduced sulfate significantly, aerosol ammonium nitrate remains high in East China. As the high nitrate abundances are strongly linked with ammonia, reducing ammonia emissions is becoming increasingly important to improve the air quality of China. Although satellite data provide evidence of substantial increases in atmospheric ammonia concentrations over major agricultural regions, long-term surface observation of ammonia concentrations are sparse. In addition, there is still no consensus on whether agricultural or non-agricultural emissions dominate the urban ammonia budget. Identifying the ammonia source by nitrogen isotope helps in designing a mitigation strategy for policymakers, but existing methods have not been well validated. Revisiting the concentration measurements and identifying source apportionment of atmospheric ammonia is thus an essential step towards reducing ammonia emissions

Metabolic risk factors of cognitive impairment in young women with major psychiatric disorder

BackgroundCognitive performance improves clinical outcomes of patients with major psychiatric disorder (MPD), but is impaired by hyperglycemia. Psychotropic agents often induce metabolism syndrome (MetS). The identification of modifiable metabolic risk factors of cognitive impairment may enable targeted improvements of patient care.ObjectiveTo investigate the relationship between MetS and cognitive impairment in young women with MPD, and to explore risk factors.MethodsWe retrospectively studied women of 18–34 years of age receiving psychotropic medications for first-onset schizophrenia (SCH), bipolar disorder (BP), or major depressive disorder (MDD). Data were obtained at four time points: presentation but before psychotropic medication; 4–8 and 8–12 weeks of psychotropic therapy; and enrollment. MATRICS Consensus Cognitive Battery, (MCCB)—based Global Deficit Scores were used to assess cognitive impairment. Multiple logistic analysis was used to calculate risk factors. Multivariate models were used to investigate factors associated with cognitive impairment.ResultsWe evaluated 2,864 participants. Cognitive impairment was observed in 61.94% of study participants, and was most prevalent among patients with BP (69.38%). HbA1c within the 8–12 week-treatment interval was the most significant risk factor and highest in BP. Factors in SCH included pre-treatment waist circumference and elevated triglycerides during the 8–12 weeks treatment interval. Cumulative dosages of antipsychotics, antidepressants, and valproate were associated with cognitive impairment in all MPD subgroups, although lithium demonstrated a protect effect (all P < 0.001).ConclusionsCognitive impairment was associated with elevated HbA1c and cumulative medication dosages. Pre-treatment waist circumference and triglyceride level at 8–12 weeks were risk factors in SCH. Monitoring these indices may inform treatment revisions to improve clinical outcomes

Quantifying the Climate Impact of the US Policy Choices Using an Economic and Earth System Model

Climate policy plays an important role in keeping global temperature rises below the target of 1.5–2 °C above pre-industrial levels, and technological innovations are key to determining the effectiveness of climate policy. In this study, we investigated the climate impact of the USA’s policy choices using the enforced multi-factor regional climate and economy system (EMRICES,) and the Earth system model from Beijing Normal University (BNU-ESM). Three emission scenarios were designed based on the assumption of whether or not the US follows its proposed nationally determined contribution (NDC) and makes use of technological innovations. The results showed that if the US does not implement the NDC and had no technological progress, there would be an extra 176.7 Gt of cumulative carbon emissions by the end of the 21st century compared to that of all the countries that follow their NDC. The additional emissions would lead to an increase of 62 ppm in CO2 concentration and a 0.4 °C increase in global warming by 2100. It would also lead to a 2% loss for the US and Chinese economies, compared to the NDC scenario. The Earth system model results also show that even if all the countries follow the DNC, it would be difficult to keep the temperature from increasing less than 1.5 °C. This study implies that the US withdrawal from the Paris Agreement and their refusal to adopt technological progress is not conducive to achieving the 1.5 °C goal, and more stringent emission reduction targets or technology innovations would be required for the world to control global warming to a level below 1.5 °C

A chlorination roasting process to extract rubidium from distinctive kaolin ore with alternative chlorinating reagent

A chlorination roasting process was proposed to recover rubidium from the distinctive rubidium-containing kaolin ore. The rubidium ions which existed in the alumina octahedron of the phyllosilicates structure could be extracted effectively by using CaCl2 as chlorinating reagent due to formation of Ca(Al2Si2O8) phase in the chlorination process of kaolin ore, and the maximum extraction yield reached 96.95%. Additionally, the kinetics results of chlorination roasting of the kaolin ore showed that the extraction rate of rubidium was controlled by the chemical reaction, and the activation energy was found to be 40.13 kJ mol(-1) in the roasting temperature range of this study. (C) 2016 Elsevier B.V. All rights reserved.</p

Section Location Technology Based on Minkowski Distance of Three-phase Asymmetric Current Components

Fault components in the fault path and the non-fault path differ due to the unbalanced distribution of positive, negative and zero-sequence components in the line in the case of a single-phase earth fault in the power distribution network. Therefore, this paper proposes an improved fault section location algorithm based on phase current, which obtains the fault component current by the subtraction of currents before and after the fault and the fundamental wave amplitude of the corresponding current through Fast Fourier Transform (FFT). Meanwhile, considering that the fault section location cannot be completed accurately with the amplitude relationship alone, the Minkowski distance calculation formula is introduced to strengthen the difference between the fault path and the non-fault path, and the relationship between the calculation results of different sections and the threshold is compared by threshold setting to complete the section location. This paper proposes the specific fault section location process and criteria and verifies the feasibility of the algorithm through simulation experiment

Determination of 31 Polycyclic Aromatic Hydrocarbons in Plant Leaves Using Internal Standard Method with Ultrasonic Extraction&ndash;Gas Chromatography&ndash;Mass Spectrometry

The method for the determination of 16 priority polycyclic aromatic hydrocarbons (PAHs) in plant leaves has been studied extensively, yet the quantitativemethod for measuring non-priority PAHs in plant leaves is limited. A method for the simultaneous determination of 31 polycyclic aromatic hydrocarbons (PAHs) in plant leaves was established using an ultrasonic extraction&ndash;gas chromatography&ndash;mass spectrometry&ndash;internal standard method. The samples of plant leaves were extracted with ultrasonic extraction and purified with solid-phase extraction columns. The PAHs were separated by using gas chromatography&ndash;mass spectrometry equipped with a DB-EUPAH capillary column (20 m &times; 0.18 mm &times; 0.14 &mu;m) with a selective ion monitoring (SIM) detection mode, and quantified with an internal standard. The method had good linearity in the range of 0.005~1.0 &mu;g/mL with correlation coefficients greater than 0.99, and the method detection limit and maximum quantitative detection limit were in the ranges of 0.2~0.7 &mu;g/kg and 0.8~2.8 &mu;g/kg, respectively. The method was verified with spiked recovery experiments. The average spiked recovery ranged from 71.0% to 97.6% and relative standard deviations (n = 6) were less than 14%. Herein, we established a quantitativemethod for the simultaneous determination of priority and non-priority PAHs in plant leaves using GC&ndash;MS. The method is highly sensitive and qualitatively accurate, and it is suitable for the determination of PAHs in plant leaves

Near-diffraction-limited Bragg reflection waveguide lasers

We report a near-diffraction-limited tapered Bragg reflection waveguide laser (BRL) with a 10 μm ridge width, which is significantly larger than the conventional design. The large mode expansion in the vertical waveguide enables a scalable increase in the ridge width for single lateral mode operation. The role of the taper angle in the performance of tapered BRLs with the intrinsic characteristics of a thick vertical waveguide was investigated. The results indicate that the BRL with a taper angle of 3° shows the best far-field performance. An extremely low vertical divergence angle of 14.5° and a lateral divergence as low as 2.8° for 95% power inclusion were realized. A continuous-wave power exceeding 1 W was demonstrated. Over the entire operating current range, the vertical beam is almost unchanged with an excellent beam quality (M2) of about 1.3. Lateral beam width increases slightly at higher currents due to the increasing contribution of side lobes, but it still remains nearly diffraction-limited with a lateral M2 of less than 2. Narrow beam divergence and high beam quality of the lasers allow simple and inexpensive collimation and coupling.National Natural Science Foundation of China (NSFC) (61474119, 61761136009, 61774153, 61774156); External Cooperation Program of Chinese Academy of Sciences (CAS) (181722KYSB20160005); Natural Science Foundation of Jilin Province (20160101243JC, 20180519024JH); China Scholarship Council (CSC) (201704910183)