Se.C.R.E.T.S.: A stability experiment on the role of segregated copper in Nb/sub 3/Sn cable-in-conduit conductors

In Se.C.R.E.T.S. (Segregated Copper Ratio Transient Stability), the stability performance under transverse field transient is compared for two Nb/sub 3/Sn cable-in-conduit conductors which differ only because of the different distribution of the stabilizing copper, either included in the Nb/sub 3/Sn strand cross section or segregated as bundled copper wires. If the segregated copper is found to be effective for stability purposes, the copper fraction in the Nb/sub 3/Sn strands can be substantially reduced, with dramatic cost advantage for the high field fusion magnets. The experiment is assembled in the SULTAN facility at CRPP, with 11 T background field and superimposed transverse pulsed field to generate the transient disturbance. The layout of the conductor, the winding sample and the assembly procedure are described. The instrumentation and the test program are planned to provide results to be easily extrapolated to the ITER conductors

Development of the Multiplex Real-Time PCR for Marburg, Ebola, and Lassa Viruses Identification

Presented are the data on the development and approbation of the method of Marburg, Ebola, and Lassa viruses identification based on real-time multiplex PCR with hybridization-fluorescent detection. This method is meant for the differential diagnostics of hemorrhagic fevers caused by these viruses. Displayed are the results of determination of multiplex PCR analytical sensitivity and specific activity

Studies of Sensitivity to Avian Flu Virus A/H5N1 in Chickens

) appear to be highly virulent for chickens. The chance of AFV infection of chickens in case of intranasal challenge is 20 times as great as in the case of peroral one, and 300 times as great as in the case of intragastral one, which bears evidence to higher sensitivity to AFV of the tissues of avian respiratory organs, in comparison with the tissues of gastro-intestinal tract. Therewith, primary target organ for virus in intranasal infected birds is their respiratory channel (mucous membrane of the nasal cavity in particular). Registered is the possibility of existence of fecal-nasal AFV transfer mechanism in chickens

Development of DNA-Biochip for Identification of Influenza A Virus Subtypes

Developed was the DNA-biochip to identify subtypes of influenza A virus, pathogenic for humans. Microchip was capable of detecting H1, H3, H5-subtypes of hemagglutinin (including H1-subtype of pandemic A/H1N1(2009) influenza virus ) and neuraminidase subtypes N1,N2 of influenza virus. This microchip was successfully tested on the strains of A/H5N1 highly pathogenic avian influenza virus, A/H1N1(2009) pandemic influenza virus, A/H1N1 and A/H3N2 seasonal influenza viruses

Dissemination of Influenza A/H5N1 Virus after Intranasal Inoculation of Chickens

Studied was dissemination of avian influenza virus (AIV) in the organism of chickens after intranasal challenge with 10-100 LD50. The primary organ of accumulation of AIV A/H5N1 (A/Chicken/Kurgan/05/2005strain) is the respiratory tract (nasal mucosa), where the virus is registered in 18 hours after challenge. The accumulation of pathogen is observed in many organs and serum of chicken in 30-32 hours after challenge. The animals die in 54 hours, the concentration of virus reaches critical value in all studied samples. The highest AIV loads (7 lg of chicken embryo infective dose - EID50/g or ml) are registered in lungs, blood serum and kidneys of chicken. The results of AIV loads measuring using titration and real time RT-PCR show high degree of correlation (r=0.89)

Anti-Helicobacter pylori activity and immunostimulatory effect of extracts from Byrsonima crassa Nied. (Malpighiaceae)

<p>Abstract</p> <p>Background</p> <p>Several <it>in vitro </it>studies have looked at the effect of medicinal plant extracts against <it>Helicobacter pylori </it>(<it>H. pylori</it>). Regardless of the popular use of <it>Byrsonima crassa </it>(<it>B. crassa</it>) as antiemetic, diuretic, febrifuge, to treat diarrhea, gastritis and ulcers, there is no data on its effects against <it>H. pylori</it>. In this study, we evaluated the anti-<it>H. pylori </it>of <it>B. crassa </it>leaves extracts and its effects on reactive oxygen/nitrogen intermediates induction by murine peritoneal macrophages.</p> <p>Methods</p> <p>The minimal inhibitory concentration (MIC) was determined by broth microdilution method and the production of hydrogen peroxide (H₂O₂) and nitric oxide (NO) by the horseradish peroxidase-dependent oxidation of phenol red and Griess reaction, respectively.</p> <p>Results</p> <p>The methanolic (MeOH) and chloroformic (CHCl₃) extracts inhibit, <it>in vitro</it>, the growth of <it>H. pylori </it>with MIC value of 1024 μg/ml. The MeOH extract induced the production H₂O₂and NO, but CHCl₃extract only NO.</p> <p>Conclusion</p> <p>Based in our results, <it>B. crassa </it>can be considered a source of compounds with anti-<it>H. pylori </it>activity, but its use should be done with caution in treatment of the gastritis and peptic ulcers, since the reactive oxygen/nitrogen intermediates are involved in the pathogenesis of gastric mucosal injury induced by ulcerogenic agents and <it>H. pylori </it>infections.</p