1 research outputs found
Ultrasmall Pt Nanoclusters as Robust Peroxidase Mimics for Colorimetric Detection of Glucose in Human Serum
In this work, a new type of ultrasmall
Pt nanoclusters (Pt NCs)
was prepared via a facile one-pot approach by using yeast extract
as the reductant and stabilizer. Besides their excellent water solubility,
these yeast extract-stabilized Pt NCs also possess attractive peroxidase
mimicking property. They can efficiently catalyze the oxidation of
3,3,5,5-tetramethylbenzidine (TMB) in the coexistence of hydrogen
peroxide (H<sub>2</sub>O<sub>2</sub>). Catalytic mechanism analysis
suggested that the peroxidase mimicking activity of these Pt NCs might
originate from their characteristic of accelerating electron transfer
between TMB and H<sub>2</sub>O<sub>2</sub>, and their enzymatic kinetics
followed typical Michaelis–Menten theory. On the basis of these
findings, we developed a new highly sensitive colorimetric method
for glucose detection, and the limit of detection was calculated as
low as 0.28 μM (S/N = 3). Further application of the present
system for glucose detection in human serum has been successfully
demonstrated, suggesting its promising utilization as robust peroxidase
mimics in the clinical diagnosis, pharmaceutical, and environmental
chemistry fields