Surface Albedo Variation and Its Influencing Factors over Dongkemadi Glacier, Central Tibetan Plateau

Glacier albedo plays a critical role in surface-atmosphere energy exchange, the variability of which influences glacier mass balance as well as water resources. Dongkemadi glacier in central Tibetan Plateau was selected as study area; this research used field measurements to verify Landsat TM-derived albedo and MOD10A1 albedo product and then analyzed the spatiotemporal variability of albedo over the glacier according to them, as well as its influence factors and the relationship with glacier mass balance. The spatial distribution of glacier albedo in winter did not vary with altitude and was determined by terrain shield, whereas, in summer, albedo increased with altitude and was only influenced by terrain shield at accumulation zone. During 2000–2009, albedo in summer decreased at a rate of 0.0052 per year and was influenced by air temperature and precipitation levels, whereas albedo in winter increased at a rate of 0.0045 per year, influenced by the level and frequency of precipitation. The annual variation of albedo in summer during 2000–2012 has the high relative to that of glacier mass balance measurement, which indicates that glacier albedo in the ablation period can be considered as a proxy for glacier mass balance

Regulation of Plant Growth and Development: A Review From a Chromatin Remodeling Perspective

In eukaryotes, genetic material is packaged into a dynamic but stable nucleoprotein structure called chromatin. Post-translational modification of chromatin domains affects the expression of underlying genes and subsequently the identity of cells by conveying epigenetic information from mother to daughter cells. SWI/SNF chromatin remodelers are ATP-dependent complexes that modulate core histone protein polypeptides, incorporate variant histone species and modify nucleotides in DNA strands within the nucleosome. The present review discusses the SWI/SNF chromatin remodeler family, its classification and recent advancements. We also address the involvement of SWI/SNF remodelers in regulating vital plant growth and development processes such as meristem establishment and maintenance, cell differentiation, organ initiation, flower morphogenesis and flowering time regulation. Moreover, the role of chromatin remodelers in key phytohormone signaling pathways is also reviewed. The information provided in this review may prompt further debate and investigations aimed at understanding plant-specific epigenetic regulation mediated by chromatin remodeling under continuously varying plant growth conditions and global climate change

Characterization of monoclonal antibodies that specifically differentiate field isolates from vaccine strains of classical swine fever virus

Classical swine fever virus (CSFV) is a major animal pathogen threatening the global pork industry. To date, numerous anti-CSFV monoclonal antibodies (mAbs) and their recognizing epitopes have been reported. However, few mAbs were systematically characterized for the capacity to differentiate field CSFV isolates from CSF vaccine strains, and the molecular basis associated with antigenic differences between vaccines and field isolates is still largely unknown. In the present study, recombinant CSFV structural glycoproteins E2 of both virulent and vaccine strains and Erns of vaccine strain were expressed using eukaryotic cells and murine mAbs generated against E2 and Erns. After serial screening and cloning of the hybridomas, the viral spectra of mAbs were respectively determined by indirect fluorescent antibody assay (IFA) using 108 CSFVs, followed by Western blot analysis using expressed glycoproteins of all CSFV sub-genotypes including vaccine strains. The antigenic structures recognized by these mAbs were characterized by epitope mapping using truncated, chimeric, and site-directed mutated E2 and Erns proteins. We have identified two vaccine-specific, one field isolate-specific, and two universal CSFV-specific mAbs and five novel conformational epitopes with critical amino acid (aa) motifs that are associated with these five mAbs: 213EPD215, 271RXGP274, and 37LXLNDG42 on E2 and 38CKGVP42, W81, and D100/V107 on Erns. Particularly, E213 of E2 is field isolate-specific, while N40 of E2 and D100/V107 of Erns are vaccine strain-specific. Results from our study further indicate that N40D of E2 mutation in field strains was likely produced under positive selection associated with long-term mass vaccination, leading to CSFV evasion of host immune response. Taking together, this study provides new insights into the antigenic structure of CSFV E2 and Erns and the differentiating mAbs will contribute to the development of a diagnostic strategy to differentiate C-strain vaccination from natural infection (DIVA) of CSFV in terms of elimination of CSF in China

Consistency of Suspended Particulate Matter Concentration in Turbid Water Retrieved from Sentinel-2 MSI and Landsat-8 OLI Sensors

Research on the consistency of suspended particulate matter (SPM) concentration retrieved from multisource satellite sensors can serve as long-time monitoring of water quality. To explore the influence of the atmospheric correction (AC) algorithm and the retrieval model on the consistency of the SPM concentration values, Landsat 8 Operational Land Imager (OLI) and Sentinel 2 MultiSpectral Imager (MSI) images acquired on the same day are used to compare the remote sensing reflectance (Rrs) SPM retrieval values in two high-turbidity lakes. An SPM retrieval model for Shengjin Lake is established based on field measurements and applied to OLI and MSI images: two SPM concentration products are highly consistent (R2 = 0.93, Root Mean Squared Error (RMSE) = 20.67 mg/L, Mean Absolute Percentage Error (MAPE) = 6.59%), and the desired results are also obtained in Chaohu Lake. Among the four AC algorithms (Management Unit of the North Seas Mathematical Models (MUMM), Atmospheric Correction for OLI’lite’(ACOLITE), Second Simulation of Satellite Signal in the Solar Spectrum (6S), Landsat 8 Surface Reflectance Code & Sen2cor (LaSRC & Sen2cor)), the two Rrs products, as well as the final SPM concentration products retrieved from OLI and MSI images, have the best consistency when using the MUMM algorithm in SeaWIFS Data Analyst System (SeaDAS) software. The consistency of SPM concentration values retrieved from OLI and MSI images using the same model or same form of models is significantly better than that retrieved by applying the optimal models with different forms

Genome-Wide Identification and Expression Profiling of CBL-CIPK Gene Family in Pineapple (Ananas comosus) and the Role of AcCBL1 in Abiotic and Biotic Stress Response

Ca2+ serves as a ubiquitous second messenger regulating several aspects of plant growth and development. A group of unique calcium sensor proteins, calcineurin B-like (CBL), interact with CBL-interacting protein kinases (CIPKs) to decode the Ca2+ signature inside the cell. Although CBL-CIPK signaling toolkit has been shown to play significant roles in the responses to numerous stresses in different plants, the information about pineapple CBL-CIPK remains obscure. In the present study, a total of eight AcCBL and 21 AcCIPK genes were identified genome-wide in pineapple. The identified genes were renamed on the basis of gene ID in ascending order and phylogenetic analysis divided into five groups. Transcriptomic data analysis showed that AcCBL and AcCIPK genes were expressed differentially in different tissues. Further, the expression analysis of AcCBL1 in different tissues showed significant changes under various abiotic stimuli. Additionally, the ectopic expression of AcCBL1 in Arabidopsis resulted in enhanced tolerance to salinity, osmotic, and fungal stress. The present study revealed the crucial contribution of the CBL-CIPK gene in various biological and physiological processes in pineapple

A Cone-Beam Computed Tomographic Study on Mandibular First Molars in a Chinese Subpopulation.

The purpose of this study was to conduct a cone-beam computed tomographic (CBCT) investigation on the root and canal configuration of the mandibular first molars, especially the morphology of the disto-lingual (DL) root, in a Chinese subpopulation. A total of 910 CBCT images of the mandibular first molars were collected from 455 patients who underwent CBCT examinations as a preoperative assessment for implants or orthodontic treatment. The following information was analyzed and evaluated: tooth position, gender, root and root canal number per tooth, root canal type of the mesial root(s) and distal root(s), angle of the DL root canal curvature, distance between two distal canal orifices in the teeth with DL root, and angle of disto-buccal canal orifice-disto-lingual canal orifice-mesio-lingual canal orifice (DB-DL-ML). Most of the mandibular first molars (64.9%, n = 591) had two roots with three root canals, and most of the mesial root canals (87.7%, n = 798) were type VI. The prevalence of the DL root was 22.1% (n = 201). The right side had a higher prevalence of DL root than the left side (p<0.05). Additionally, the curvature of the DL root canal were greater in the bucco-lingual (BL) orientation (30.10°±14.02°) than in the mesio-distal (MD) orientation (14.03°± 8.56°) (p<0.05). Overall there was a high prevalence of DL root in the mandibular first molars, and most of the DL roots were curved in different degrees. This study provided detailed information about the root canal morphology of the mandibular first molars in a Chinese subpopulation

The angle of the DB-DL-ML.

<p>The three pictures showed the angles of different degrees. D, distal side; M, mesial side; L, lingual side; B, buccal side.</p