Antioxidation of NAC in cell death induced by T-2 toxin.

<p><b>A</b>. Antioxidation of NAC in the cell death process of Hela cells. <b>B</b>. Endogenous GSH level of Hela under protection of NAC. Data was presented as mean ± SD. *p<0.05, **p<0.01, ***p<0.001.</p

The effect of up-regulated JunD on cells.

<p><b>A</b>. The expression levels of JunD induced by T-2 toxin. <b>B</b>. The expression of JunD after transfection of JunD over-expression vector in three cell strains. wt: wild type. +: cell strains transfected with over-expression vector. <b>C</b>. The effect of over-expressed JunD on cell survival rate. T-2: wild-type cells under T-2 toxin stress. JunD (+/+): JunD over-expressied cell lines under T-2 toxin stress. Data was presented as mean ± SD. *p<0.05, **p<0.01, ***p<0.001.</p

Western-blot analysis on activated Caspase-8 and Caspase-9 level.

<p><b>A</b>. Activated Caspase-8 and Caspase-9 level in Hela cells when treated with T-2 toxin at the concentration of LC50 for 8, 16, and 24 h respectively. <b>B</b>. Activated Caspase-8 and Caspase-9 level in Changliver cells were treated with T-2 toxin. <b>C</b>. Bel -7402 cells were treated with T-2 toxin.</p

Activation of Caspase-3 induced by T-2 toxin.

<p><b>A</b>. Detection of Caspase-3 hydrolase activity under T-2 toxin stress. <b>B</b>. Western blot result of Caspase-3 activated fragments in Hela cells when treated with T-2 at the concentration of LC50 for 8, 16, and 24 h respectively. <b>C</b>. Western blot result of Caspase-3 activated fragments in Changliver cells. <b>D</b>. Caspase-3 activity in Bel -7402 cell. Data was presented as mean ± SD. *p<0.05, **p<0.01, ***p<0.001.</p

Intracellular redox level under T-2 toxin.

<p>A. Level of endogenous GSH under T-2 toxin stress. B. Levels of endogenous MDA under T-2 toxin stress. Data was presented as mean ± SD. *p<0.05, **p<0.01, ***p<0.001.</p

The inhibition effect of T-2 toxin on three cell strains.

<p><b>A</b>. Dose-dependent inhibition ration of T-2 toxin at 24 h. <b>B</b>. Time-dependent effect of T-2 toxin to cells. Data was presented as mean ± SD. *p<0.05, **p<0.01, ***p<0.001.</p

Percentage of positive PBMC after stimulation with PHA in the absence or presence of 3 types of treated MSCs.

<p>After reduction of LLL treated MSCs, CD25+ cells decreased (**P < 0.01 vs. PHA) and CD69+ cells were also reduced compared to control groups (**P < 0.01 vs. PHA).</p

LLL decrease nuclear translocation of NF-κB.

<p>The images show the cytoplasmic localization of NF-κB in the control cells (upper panel), the nuclear translocation of NF-κB in cells treated with LPS (middle panel) and LLL treatment blocked the nuclear translocation of NF-κB caused by LPS stimulation (lower panel).</p

Primers used in study for PCR.

<p>Primers used in study for PCR.</p

LLL suppress ROS and NO promotion induced by LPS in MSCs.

<p>MSCs were incubated with LPS (10ng/mL) and simultaneous treated with LLL for 1 h. Error bars represent the mean±SD (n = 3 per group). Values of **P<0.01 vs. LPS and **P<0.01 vs. control were considered statistically significant.</p