Phylogeny of the genus Morus (Urticales: Moraceae) inferred from ITS and trnL-F sequences

Both nuclear ribosomal ITS and chloroplast trnL-F sequences were acquired from 13 mulberry genotypes belonging to nine species and three varieties, and one paper mulberry. The later belongs to genus B. papyrifera, designed as outgroup, and were analyzed. Within the genus Morus, the sequence diversity of ITS was much higher than that of trnL-F. The results of phylogenetic analyses based on these data (separately or combined) show that the genus Morus is monophyletic group. Strict consensus tree obtained through the Neighbor-joining method can be divided into five major clades in the genus Morus, according to combined sequence data. M. bombycis, M. alba var. venose formed clades A and B, respectively. Clade C comprises of 5 species; M. rotundiloba, M. atropurpurea, M. mongolica, M. australi, and M. mongolica var. diabolica. Clade D comprises of 3 species; M. wittiorum, M. laevigata, and M. alba. Clade E comprises of 2 species; M. multicaulis, and M.alba var. macrophylla. The results from cluster analysis were basically in agreement with the existing morphologic classification.African Journal of Biotechnology Vol. 4 (6), pp. 563-569, 200

Insect-Specific microRNA Involved in the Development of the Silkworm Bombyx mori

MicroRNAs (miRNAs) are endogenous non-coding genes that participate in post-transcription regulation by either degrading mRNA or blocking its translation. It is considered to be very important in regulating insect development and metamorphosis. We conducted a large-scale screening for miRNA genes in the silkworm Bombyx mori using sequence-by-synthesis (SBS) deep sequencing of mixed RNAs from egg, larval, pupal, and adult stages. Of 2,227,930 SBS tags, 1,144,485 ranged from 17 to 25 nt, corresponding to 256,604 unique tags. Among these non-redundant tags, 95,184 were matched to the silkworm genome. We identified 3,750 miRNA candidate genes using a computational pipeline combining RNAfold and TripletSVM algorithms. We confirmed 354 miRNA genes using miRNA microarrays and then performed expression profile analysis on these miRNAs for all developmental stages. While 106 miRNAs were expressed in all stages, 248 miRNAs were egg- and pupa-specific, suggesting that insect miRNAs play a significant role in embryogenesis and metamorphosis. We selected eight miRNAs for quantitative RT-PCR analysis; six of these were consistent with our microarray results. In addition, we searched for orthologous miRNA genes in mammals, a nematode, and other insects and found that most silkworm miRNAs are conserved in insects, whereas only a small number of silkworm miRNAs has orthologs in mammals and the nematode. These results suggest that there are many miRNAs unique to insects

Retrieval of Soil Moisture Content Based on Multisatellite Dual-Frequency Combination Multipath Errors

Global navigation satellite system interferometric reflectometry (GNSS-IR) is a new type of microwave remote sensing technology that can measure soil moisture content (SMC). GNSS-IR soil moisture retrieval methods based on the satellite signal-to-noise ratio (SNR) and triple-frequency signal combination have the following shortcomings: SNR does not always exist in the original GNSS file, and the number of triple-frequency signal observation satellites is small, resulting in GNSS-IR soil moisture observation time resolution being low. Based on the above problems, in this study, we constructed a soil moisture inversion method based on multisatellite dual-frequency combined multipath error is proposed: the multipath error calculation model of dual-frequency carrier phase (L4 Ionosphere Free, L4_IF) and dual-frequency pseudorange (DFP) without ionospheric effect is constructed. We selected the data of the five epochs before and after the time point of the effective satellite period to construct the multipath error model and error equation, and we solved the delay phase for soil moisture retrieval. We verified the method using Plate Boundary Observatory (PBO) P041 site data. The results showed that the Pearson correlation coefficients (R) of L4_IF and DFP methods at P041 station are 0.97 and 0.91, respectively. To better verify the results’ reliability and the proposed method’s effectiveness, the soil moisture data of the MFLE station about 210 m away from P041 station are used as the verification data in this paper. The results showed that the delay phase solved by multipath error and soil moisture strongly correlate. Pearson correlation coefficients (R) of L4_IF and DFP methods at MFLE station are 0.93 and 0.86, respectively. In order to improve the inversion accuracy of GNSS-IR soil moisture, this paper constructs the prediction model of soil moisture by using the linear regression (ULR), back propagation neural network (BPNN) and radial basis function neural network (RBFNN), and evaluates the accuracy of each model. The results showed that the soil moisture retrieval method based on multisatellite dual-frequency combined multipath error can replace the traditional retrieval method and effectively improve the time resolution of GNSS-IR soil moisture estimation. To perform highly dynamic monitoring of soil moisture, higher retrieval accuracy can only be obtained with a small epoch multipath error

Balance of Macrophage Activation by a Complex Coacervate-Based Adhesive Drug Carrier Facilitates Diabetic Wound Healing

Uncontrolled and sustained inflammation disrupts the wound-healing process and produces excessive reactive oxygen species, resulting in chronic or impaired wound closure. Natural antioxidants such as plant-based extracts and natural polysaccharides have a long history in wound care. However, they are hard to apply to wound beds due to high levels of exudate or anatomical sites to which securing a dressing is difficult. Therefore, we developed a complex coacervate-based drug carrier with underwater adhesive properties that circumvents these challenges by enabling wet adhesion and controlling inflammatory responses. This resulted in significantly accelerated wound healing through balancing the pro- and anti-inflammatory responses in macrophages. In brief, we designed a complex coacervate-based drug carrier (ADC) comprising oligochitosan and inositol hexaphosphate to entrap and release antioxidant proanthocyanins (PA) in a sustained way. The results from in vitro experiments demonstrated that ADC is able to reduce LPS-stimulated pro-inflammatory responses in macrophages. The ability of ADC to reduce LPS-stimulated pro-inflammatory responses in macrophages is even more promising when ADC is encapsulated with PA (ADC-PA). Our results indicate that ADC-PA is able to polarize macrophages into an M2 tissue-healing phenotype via up-regulation of anti-inflammatory and resolution of inflammatory responses. Treatment with ADC-PA around the wound beds fine-tunes the balance between the numbers of inducible nitric oxide synthase-positive (iNOS+) and mannose receptor-negative (CD206-) M1 and iNOS-CD206+ M2 macrophages in the wound microenvironment compared to controls. Achieving such a balance between the numbers of iNOS+CD206- M1 and iNOS-CD206+ M2 macrophages in the wound microenvironment has led to significantly improved wound closure in mouse models of diabetes, which exhibit severe impairments in wound healing. Together, our results demonstrate for the first time the use of a complex coacervate-based drug delivery system to promote timely resolution of the inflammatory responses for diabetic wound healing by fine-tuning the functions of macrophages