Pro-fibrinolysis mediators and antimicrobial peptide in epithelial cell supernatant.

<p>Pro-fibrinolysis mediators and antimicrobial peptide in epithelial cell supernatant.</p

Schematic experimental flow of platelet activation and aggregation, and epithelial cell adhesion assays.

<p>Schematic of experimental flow of platelet activation and aggregation, and epithelial cell adhesion assays.</p

Cytokines in the supernatant of PRP cultured on titanium surfaces.

<p>Cytokines in the supernatant of PRP cultured on titanium surfaces.</p

SEM ultrastructure of PRP and whole blood aggregation on the titanium surfaces.

<p>PRP (a,b) and whole blood (c,d) were incubated for 60 minutes on the titanium surfaces with untreated control (a, c) and PAR4-AP coating (b,d). SEM micrographs were taken at the magnification of 10,000x. Supplemental data is published in <a href="http://dx.doi.org/10.7910/DVN/FDMPZY" target="_blank">http://dx.doi.org/10.7910/DVN/FDMPZY</a>.</p

SEM ultrastructure of OBA9 epithelial cells on the PRP pre-incubated titanium surfaces.

<p>Representative SEM micrographs of OBA9 epithelial cells on the untreated control (a) and PAR4-AP coated titanium (b,c) surfaces at the magnifications 500x (a,b) and 5000x (c). Supplemental data is published in <a href="http://dx.doi.org/10.7910/DVN/FDMPZY" target="_blank">http://dx.doi.org/10.7910/DVN/FDMPZY</a>.</p

Adhesion of OBA9 epithelial cells to PRP pre-incubated titanium surfaces.

<p>Adhered OBA9 cells were counted by nuclear DNA staining with Hoechst 33342. Each data point represents mean ± SD of three independent cultures. Kinetic adhesion was determined in the titanium that was pre-incubated with PRP from one representative donor (a). The adhesion assay was triplicated at 60 minutes with PRP from different donors (b). ** indicates a significant difference than control at the corresponding time point and in the corresponding donors (**<i>p < 0</i>.<i>01</i>). Supplemental data is published in <a href="http://dx.doi.org/10.7910/DVN/FDMPZY" target="_blank">http://dx.doi.org/10.7910/DVN/FDMPZY</a>.</p

Pro-fibrinolysis mediators and antimicrobial peptide in epithelial cell supernatant.

<p>Pro-fibrinolysis mediators and antimicrobial peptide in epithelial cell supernatant.</p

Quantitation of PRP and whole blood aggregation on the titanium surfaces.

<p>PRP and whole blood aggregation was quantified by total cellular ATP in a time course at 1, 5, 15, 30 and 60 minutes (a,b) and at a plateaued time point of 60 min (c,d). Values are mean ± SD of three independent cultures of PRP and whole blood from one representative donor (a,b) and three independent donors (c,d). ** indicates a significant difference with control at the corresponding time point (**, <i>p < 0</i>.<i>01</i>). Supplemental data is published in <a href="http://dx.doi.org/10.7910/DVN/FDMPZY" target="_blank">http://dx.doi.org/10.7910/DVN/FDMPZY</a>.</p