Exploration of Ideological and Political Education in the Course of “Biopharmaceutical Testing and Testing Technology” under the Post Pandemic Situation

Motivated by curriculum reform and the traditional theoretical teaching content system of drug analysis and testing, we will strengthen experimental teaching in accordance with enterprise needs of vocational colleges. And then, under the direction of content and task driven, we will build an ACQUIN quality certification system for the Biopharmaceutical Testing and Testing Technology course to strengthen students’ independent participation, pique their interest in learning, and cultivate high-quality skilled talents who are capable of doing things and being good person, by thoroughly examining the ideological and political components of the course

Pharmacologic Stem Cell Based Intervention as a New Approach to Osteoporosis Treatment in Rodents

Background: Osteoporosis is the most prevalent skeletal disorder, characterized by a low bone mineral density (BMD) and bone structural deterioration, leading to bone fragility fractures. Accelerated bone resorption by osteoclasts has been established as a principal mechanism in osteoporosis. However, recent experimental evidences suggest that inappropriate apoptosis of osteoblasts/osteocytes accounts for, at least in part, the imbalance in bone remodeling as occurs in osteoporosis. The aim of this study is to examine whether aspirin, which has been reported as an effective drug improving bone mineral density in human epidemiology studies, regulates the balance between bone resorption and bone formation at stem cell levels. Methods and Findings: We found that T cell-mediated bone marrow mesenchymal stem cell (BMMSC) impairment plays a crucial role in ovariectomized-induced osteoporosis. Ex vivo mechanistic studied revealed that T cell-mediated BMMSC impairment was mainly attributed to the apoptosis of BMMSCs via the Fas/Fas ligand pathway. To explore potential of using pharmacologic stem cell based intervention as an approach for osteoporosis treatment, we selected ovariectomy (OVX)-induced osteoporosis mouse model to examine feasibility and mechanism of aspirin-mediated therapy for osteoporosis. We found that aspirin can inhibit T cell activation and Fas ligand induced BMMSC apoptosis in vitro. Further, we revealed that aspirin increases osteogenesis of BMMSCs by aiming at telomerase activity and inhibits osteoclast activity in OVX mice, leading to ameliorating bone density. Conclusion: Our findings have revealed a novel osteoporosis mechanism in which activated T cells induce BMMSC apoptosis via Fas/Fas ligand pathway and suggested that pharmacologic stem cell based intervention by aspirin may be a new alternative in osteoporosis treatment including activated osteoblasts and inhibited osteoclasts. © 2008 Yamaza et al

Genome-wide identification and analysis of heterotic loci in three maize hybrids

Heterosis, or hybrid vigour, is a predominant phenomenon in plant genetics, serving as the basis of crop hybrid breeding, but the causative loci and genes underlying heterosis remain unclear in many crops. Here, we present a large-scale genetic analysis using 5360 offsprings from three elite maize hybrids, which identifies 628 loci underlying 19 yield-related traits with relatively high mapping resolutions. Heterotic pattern investigations of the 628 loci show that numerous loci, mostly with complete–incomplete dominance (the major one) or overdominance effects (the secondary one) for heterozygous genotypes and nearly equal proportion of advantageous alleles from both parental lines, are the major causes of strong heterosis in these hybrids. Follow-up studies for 17 heterotic loci in an independent experiment using 2225 F2 individuals suggest most heterotic effects are roughly stable between environments with a small variation. Candidate gene analysis for one major heterotic locus (ub3) in maize implies that there may exist some common genes contributing to crop heterosis. These results provide a community resource for genetics studies in maize and new implications for heterosis in plants

Pharmacologic stem cell based intervention as a new approach to osteoporosis treatment in rodents

Background: Osteoporosis is the most prevalent skeletal disorder, characterized by a low bone mineral density (BMD) and bone structural deterioration, leading to bone fragility fractures. Accelerated bone resorption by osteoclasts has been established as a principal mechanism in osteoporosis. However, recent experimental evidences suggest that inappropriate apoptosis of osteoblasts/osteocytes accounts for, at least in part, the imbalance in bone remodeling as occurs in osteoporosis. The aim of this study is to examine whether aspirin, which has been reported as an effective drug improving bone mineral density in human epidemiology studies, regulates the balance between bone resorption and bone formation at stem cell levels. Methods and Findings: We found that T cell-mediated bone marrow mesenchymal stem cell (BMMSC) impairment plays a crucial role in ovariectomized-induced osteoporosis. Ex vivo mechanistic studies revealed that T cell-mediated BMMSC impairment was mainly attributed to the apoptosis of BMMSCs via the Fas/Fas ligand pathway. To explore potential of using pharmacologic stem cell based intervention as an approach for osteoporosis treatment, we selected ovariectomy (OVX)- induced ostoeporosis mouse model to examine feasibility and mechanism of aspirin-mediated therapy for osteoporosis. We found that aspirin can inhibit T cell activation and Fas ligand induced BMMSC apoptosis in vitro. Further, we revealed that aspirin increases osteogenesis of BMMSCs by aiming at telomerase activity and inhibits osteoclast activity in OVX mice, leading to ameliorating bone density. Conclusion: Our findings have revealed a novel osteoporosis mechanism in which activated T cells induce BMMSC apoptosis via Fas/Fas ligand pathway and suggested that pharmacologic stem cell based intervention by aspirin may be a new alternative in osteoporosis treatment including activated osteoblasts and inhibited osteoclasts.Takayoshi Yamaza, Yasuo Miura, Yanming Bi, Yongzhong Liu, Kentaro Akiyama, Wataru Sonoyama, Voymesh Patel, Silvio Gutkind, Marian Young, Stan Gronthos, Anh Le, Cun-Yu Wang, WanJun Chen and Songtao Sh

Hopf Bifurcation and Control of a Fractional-Order Delay Stage Structure Prey-Predator Model with Two Fear Effects and Prey Refuge

A generalized delay stage structure prey-predator model with fear effect and prey refuge is considered in this paper via introducing fractional-order and fear effect induced by immature predators. Hopf bifurcation and control of this system are investigated though regarding the delay as the parameter. Firstly, by using the method of linearization and Laplace transform, the roots of the characteristic equation of the linearized system of the original system are discussed, and the sufficient conditions for the system exhibits an unstable state of symmetrical periodic oscillation (Hopf bifurcation) are explored. Secondly, a linear delay feedback controller is added to the system to increase the stability domain successfully. Thirdly, numerical simulations are performed to validate the theoretical analysis, and the various impacts on the dynamical behavior of the system occurring by fear effects, prey refuge, and each fractional-order are illustrated, respectively. Furthermore, the influence of feedback gain on the bifurcation critical point is analyzed. Finally, an analysis based on the results and in-depth research about this system under the biological background is stated in the conclusion

Risk factors of post-traumatic stress disorder (PTSD) after Wenchuan earthquake: a case control study.

BACKGROUND: Few clues were found in the literature about the independent risk factors for PTSD among earthquake survivors in Sichuan province three years after the 2008 earthquake. Ours was the first case-control study with matching factors of age and distance from the epicenter among survivors age 16 years or older, three years after the catastrophe. OBJECTIVES: To identify independent risk factors for PTSD among earthquake survivors. METHODS: We performed a population-based matched case-control study. The cases were drawn from earthquake areas three years after the Wenchuan earthquake, including 113 cases who met positive criteria for PTSD symptoms according to the PCL-C (PTSD Checklist-Civilian Version) score and 452 controls who did not meet the criteria. Cases and controls were matched individually by birth year (+ three years) and the town they lived in when the earthquake occurred. RESULTS: Independent risk factors for PTSD symptoms included two-week disease prevalence (odds ratio [OR],1.92; 95% confidence interval [CI],1.18-3.13), witnessing someone being killed in the earthquake (OR, 2.04;95%CI, 1.17-3.58), having no regular income after the earthquake (OR, 0.52; 95%CI, 0.28-0.98), receiving mental health support only one time after the earthquake (OR, 2.43; 95%CI, 1.09-5.42) and lower social support (lower PSSS score) (OR, 0.95; 95%CI, 0.93-0.97). CONCLUSION: Earthquake experience, suffering from physical illnesses, lack of stable income, and lower social support were associated with PTSD symptoms

Study on Dynamic Failure Characteristics of Coal and Rock in Stope Induced by Dynamic Load of Steep Horizontal Sublevel Mining

In this paper, through a similar simulation experiment, the roof rock breaking situation of horizontal sublevel caving method in extremely thick and steep inclined coal seam is studied, and the response characteristics of coal and rock mass under different dynamic load strength are analyzed. The mechanical response mechanisms of different mining positions under the influence of dynamic load and the law characteristics of the surrounding rock from elastic deformation to impact failure are revealed. The study shows that with the increase in the horizontal sublevel mining depth, the roof is gradually broken from the suspended state, and the broken block fills the goaf. The thin rock strata form a granular structure, and the thick rock strata form a block splicing and occlusion structure. On the same side of the coal seam floor, the concentrated stress gradually increases, and the closer the distance from the goaf is, the more obvious the stress change in the coal body below is. With the increase in dynamic load energy, the dynamic instability and failure of the dynamic load side of the roadway occur, and the stress of the bottom coal in the intake roadway of the working face increases due to the influence of the lateral abutment pressure of the goaf, while the stress of the bottom coal in the middle of the working face and the return airway decreases due to the mining of the upper section, which reveals that the dynamic load-induced dynamic behavior requires the critical energy. Due to vibration waves, the dynamic load effect is short, and the reflective stretching is generated on the surface of the roadway, resulting in the failure of the roadway. The peak acceleration increases linearly with the increase in source energy, indicating that the stronger the dynamic load energy is, the higher the impact risk is. When the dynamic load intensity is constant, the peak acceleration decreases with the increase in propagation distance, indicating that the vibration wave has the dominant propagation direction, and there are certain directional differences in the effect of coal and rock. When the dynamic load is applied, the impact failure of the coal body has a critical displacement. When the displacement of the roadway surface is less than this critical displacement, the impact failure will not occur

Boron deficiency alters root growth and development and interacts with auxin metabolism by influencing the expression of auxin synthesis and transport genes

Boron (B) deficiency inhibits and disturbs root growth and development by interacting with auxin (indole-3-acetic acid, IAA). However, the underlying mechanism of this interaction is still poorly understood. This study found that plants in a long-term boron deprivation treatment (∼0.25 µg L−1) had inhibited elongation of trifoliate orange roots, enlarged root tips and severe necrosis as well as a significant decrease in soluble boron and IAA content in seedling root tips. The results of a short-term boron deprivation treatment showed that a significant decrease in soluble boron and IAA content occurred after 3 hours of treatment (HOT) and 1 day of treatment (DOT), respectively. Moreover, the expression of IAA synthetic genes (TAA1, TAR2, YUC3 and YUC8) was strongly induced as early as 3 HOT and was then significantly reduced. The expression of rootward IAA transport genes (AUX1, PIN1 and PIN4) decreased significantly in the boron deprivation treatment, but the expression levels of shootward IAA transport genes (LAX1, ABCB1 and PIN3) were significantly increased. Taken together, the increase in IAA content before 1 DOT may be due to increased IAA synthesis caused by the induction of TAA1, TAR2, YUC3 and YUC8 expression; the subsequent decrease and the significantly lower final IAA content compared to the +B treatment (0.25 mg L−1 B) may be due to the reduced expression of IAA synthetic genes and rootward IAA transport genes and the increased expression of shootward IAA transport genes. Abbreviations: DAT: days after treatment; DOT: day of treatment; FW: fresh weight; HOT: hour of treatment; isB: insoluble boron; LC: liquid chromatography; LSD: least-significant difference; MS: mass spectrometry; PAT: polar auxin transport; qRT-PCR: quantitative real-time polymerase chain reaction; sB: soluble boro