145 research outputs found
Bounded-Distortion Metric Learning
Metric learning aims to embed one metric space into another to benefit tasks
like classification and clustering. Although a greatly distorted metric space
has a high degree of freedom to fit training data, it is prone to overfitting
and numerical inaccuracy. This paper presents {\it bounded-distortion metric
learning} (BDML), a new metric learning framework which amounts to finding an
optimal Mahalanobis metric space with a bounded-distortion constraint. An
efficient solver based on the multiplicative weights update method is proposed.
Moreover, we generalize BDML to pseudo-metric learning and devise the
semidefinite relaxation and a randomized algorithm to approximately solve it.
We further provide theoretical analysis to show that distortion is a key
ingredient for stability and generalization ability of our BDML algorithm.
Extensive experiments on several benchmark datasets yield promising results
Current insights in the preclinical study of palatal wound healing and oronasal fistula after cleft palate repair
Poor palatal wound healing after cleft palate repair could lead to unfavorable prognosis such as oronasal fistula (ONF), which might affect the patient’s velopharyngeal function as well as their quality of life. Thus, restoring poor palatal wound healing for avoiding the occurrence of ONF should be considered the key to postoperative care after cleft palate repair. This review provided current insights in the preclinical study of poor palatal wound healing after cleft palate repair. This review comprehensively introduced the animal model establishment for palatal wound healing and related ONF, including the models by mice, rats, piglets, and dogs, and then demonstrated the aspects for investigating poor palatal wound healing and related treatments, including possible signaling pathways that could be involved in the formation of poor palatal wound healing, the related microbiota changes because of the deformity of palatal structure, and the studies for potential therapeutic strategies for palatal wound healing and ONF. The purpose of this review was to show the state of the art in preclinical studies about palatal wound healing after cleft palate repair and to show the promising aspects for better management of palatal wound healing
ChatGPT for Shaping the Future of Dentistry: The Potential of Multi-Modal Large Language Model
The ChatGPT, a lite and conversational variant of Generative Pretrained
Transformer 4 (GPT-4) developed by OpenAI, is one of the milestone Large
Language Models (LLMs) with billions of parameters. LLMs have stirred up much
interest among researchers and practitioners in their impressive skills in
natural language processing tasks, which profoundly impact various fields. This
paper mainly discusses the future applications of LLMs in dentistry. We
introduce two primary LLM deployment methods in dentistry, including automated
dental diagnosis and cross-modal dental diagnosis, and examine their potential
applications. Especially, equipped with a cross-modal encoder, a single LLM can
manage multi-source data and conduct advanced natural language reasoning to
perform complex clinical operations. We also present cases to demonstrate the
potential of a fully automatic Multi-Modal LLM AI system for dentistry clinical
application. While LLMs offer significant potential benefits, the challenges,
such as data privacy, data quality, and model bias, need further study.
Overall, LLMs have the potential to revolutionize dental diagnosis and
treatment, which indicates a promising avenue for clinical application and
research in dentistry
Mutations in TUBB8 and Human Oocyte Meiotic Arrest
BACKGROUND Human reproduction depends on the fusion of a mature oocyte with a sperm cell to form a fertilized egg. The genetic events that lead to the arrest of human oocyte maturation are unknown.
METHODS We sequenced the exomes of five members of a four-generation family, three of whom had infertility due to oocyte meiosis I arrest. We performed Sanger sequencing of a candidate gene, TUBB8, in DNA samples from these members, additional family members, and members of 23 other affected families. The expression of TUBB8 and all other β-tubulin isotypes was assessed in human oocytes, early embryos, sperm cells, and several somatic tissues by means of a quantitative reverse- transcriptase–polymerase-chain-reaction assay. We evaluated the effect of the TUBB8 mutations on the assembly of the heterodimer consisting of one α-tubulin polypeptide and one β-tubulin polypeptide (α/β-tubulin heterodimer) in vitro, on microtubule architecture in HeLa cells, on microtubule dynamics in yeast cells, and on spindle assembly in mouse and human oocytes.
RESULTS We identified seven mutations in the primate-specific gene TUBB8 that were responsible for oocyte meiosis I arrest in 7 of the 24 families. TUBB8 expression is unique to oocytes and the early embryo, in which this gene accounts for almost all the expressed β-tubulin. The mutations affect chaperone-dependent folding and assembly of the α/β-tubulin heterodimer, disrupt microtubule behavior on expression in cultured cells, alter microtubule dynamics in vivo, and cause catastrophic spindle-assembly defects and maturation arrest on expression in mouse and human oocytes.
CONCLUSIONS TUBB8 mutations have dominant-negative effects that disrupt microtubule behavior and oocyte meiotic spindle assembly and maturation, causing female infertility. (Funded by the National Basic Research Program of China and others.
Enhancement of stress tolerance in transgenic tobacco plants constitutively expressing AtIpk2β, an inositol polyphosphate 6-/3-kinase from Arabidopsis thaliana
Inositol phosphates (IPs) and their turnover products have been implicated to play important roles in stress signaling in eukaryotic cells. In higher plants genes encoding inositol polyphosphate kinases have been identified previously, but their physiological functions have not been fully resolved. Here we expressed Arabidopsis inositol polyphosphate 6-/3-kinase (AtIpk2β) in two heterologous systems, i.e. the yeast Saccharomycescerevisiae and in tobacco (Nicotiana tabacum), and tested the effect on abiotic stress tolerance. Expression of AtIpk2β rescued the salt-, osmotic- and temperature-sensitive growth defects of a yeast mutant strain (arg82Δ) that lacks inositol polyphosphate multikinase activity encoded by the ARG82/IPK2 gene. Transgenic tobacco plants constitutively expressing AtIpk2β under the control of the Cauliflower Mosaic Virus 35S promoter were generated and found to exhibit improved tolerance to diverse abiotic stresses when compared to wild type plants. Expression patterns of various stress responsive genes were enhanced, and the activities of anti-oxidative enzymes were elevated in transgenic plants, suggesting a possible involvement of AtIpk2β in plant stress responses
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