Chicken Embryos as a Potential New Model for Early Onset Type I Diabetes

Diabetic retinopathy (DR) is the leading cause of blindness among the American working population. The purpose of this study is to establish a new diabetic animal model using a cone-dominant avian species to address the distorted color vision and altered cone pathway responses in prediabetic and early diabetic patients. Chicken embryos were injected with either streptozotocin (STZ), high concentration of glucose (high-glucose), or vehicle at embryonic day 11. Cataracts occurred in varying degrees in both STZ- and high glucose-induced diabetic chick embryos at E18. Streptozotocin-diabetic chicken embryos had decreased levels of blood insulin, glucose transporter 4 (Glut4), and phosphorylated protein kinase B (pAKT). In STZ-injected E20 embryos, the ERG amplitudes of both a- and b-waves were significantly decreased, the implicit time of the a-wave was delayed, while that of the b-wave was significantly increased. Photoreceptors cultured from STZ-injected E18 embryos had a significant decrease in L-type voltage-gated calcium channel (L-VGCC) currents, which was reflected in the decreased level of L-VGCCα1D subunit in the STZ-diabetic retinas. Through these independent lines of evidence, STZ-injection was able to induce pathological conditions in the chicken embryonic retina, and it is promising to use chickens as a potential new animal model for type I diabetes

Chicken Embryos as a Potential New Model for Early Onset Type I Diabetes

Diabetic retinopathy (DR) is the leading cause of blindness among the American working population. The purpose of this study is to establish a new diabetic animal model using a cone-dominant avian species to address the distorted color vision and altered cone pathway responses in prediabetic and early diabetic patients. Chicken embryos were injected with either streptozotocin (STZ), high concentration of glucose (high-glucose), or vehicle at embryonic day 11. Cataracts occurred in varying degrees in both STZ- and high glucose-induced diabetic chick embryos at E18. Streptozotocin-diabetic chicken embryos had decreased levels of blood insulin, glucose transporter 4 (Glut4), and phosphorylated protein kinase B (pAKT). In STZ-injected E20 embryos, the ERG amplitudes of both a- and b-waves were significantly decreased, the implicit time of the a-wave was delayed, while that of the b-wave was significantly increased. Photoreceptors cultured from STZ-injected E18 embryos had a significant decrease in L-type voltage-gated calcium channel (L-VGCC) currents, which was reflected in the decreased level of L-VGCCα1D subunit in the STZ-diabetic retinas. Through these independent lines of evidence, STZ-injection was able to induce pathological conditions in the chicken embryonic retina, and it is promising to use chickens as a potential new animal model for type I diabetes

Deletion of miR-150 Exacerbates Retinal Vascular Overgrowth in High-Fat-Diet Induced Diabetic Mice

Diabetic retinopathy (DR) is the leading cause of blindness among American adults above 40 years old. The vascular complication in DR is a major cause of visual impairment, making finding therapeutic targets to block pathological angiogenesis a primary goal for developing DR treatments. MicroRNAs (miRs) have been proposed as diagnostic biomarkers and potential therapeutic targets for various ocular diseases including DR. In diabetic animals, the expression levels of several miRs, including miR-150, are altered. The expression of miR-150 is significantly suppressed in pathological neovascularization in mice with hyperoxia-induced retinopathy. The purpose of this study was to investigate the functional role of miR-150 in the development of retinal microvasculature complications in high-fat-diet (HFD) induced type 2 diabetic mice. Wild type (WT) and miR-150 null mutant (miR-150-/-) male mice were given a HFD (59% fat calories) or normal chow diet. Chronic HFD caused a decrease of serum miR-150 in WT mice. Mice on HFD for 7 months (both WT and miR-150-/-) had significant decreases in retinal light responses measured by electroretinograms (ERGs). The retinal neovascularization in miR-150-/--HFD mice was significantly higher compared to their age matched WT-HFD mice, which indicates that miR-150 null mutation exacerbates chronic HFD-induced neovascularization in the retina. Overexpression of miR-150 in cultured endothelial cells caused a significant reduction of vascular endothelial growth factor receptor 2 (VEGFR2) protein levels. Hence, deletion of miR-150 significantly increased the retinal pathological angiogenesis in HFD induced type 2 diabetic mice, which was in part through VEGFR2

Micro-displacement reconstruction using a laser self-mixing grating interferometer with multiple-diffraction

In this paper, we demonstrated an improved laser self-mixing grating interferometer (SMGI) with auto-collimation design which can avoid the disturbance from the light feedback of the zero-order diffraction beam. In order to obtain higher optical subdivision, SMGI with multiple-diffraction is implemented. Both theoretical analysis and experimental work show that the proposed system for displacement measurement can achieve high sensitivity and low measurement uncertainty. Using the proposed system, different forms of micro-displacement signals applied on the target (grating) have been reconstructed with accuracy of a few nanometers. The work presented in this paper provides a good way to achieve robust and high precision measurement with compact system configuration

Fault reconstruction for Markovian jump systems with iterative adaptive observer

This paper investigates the fault observer design problem for Markovian jump systems with simultaneous time-varying actuator efficiency factors, additive actuator and sensor faults. Two types of adaptive observer methods are developed to solve the investigated design problem. The first one refers an adaptive fault observer, which can reconstruct the states and faults through the online adaptive mechanism. The second one is an iterative adaptive observer, where the iterative mean estimations can approximate to the states, actuator efficiency factors, additive actuator and sensor faults simultaneously. In both two methods, the sliding surface switching problem for jumping systems in sliding mode observer approaches is avoided. Finally, an F-404 aircraft engine system is exploited to demonstrate the effectiveness of the proposed new design techniques.Liheng Chen, Peng Shi, Ming Li

Experimental and Numerical Studies on Vertical Properties of a New Multi-Dimensional Earthquake Isolation and Mitigation Device

When designing critical structures such as long-span structures and high-rise buildings, earthquake excitation in the vertical direction, in addition to the horizontal direction, should also be taken into consideration. Study on new devices that can mitigate and isolate multi-dimensional (including both horizontal and vertical) earthquake actions has a remarkable significance. A new kind of multi-dimensional earthquake isolation and mitigation device was recently developed, and experimental study on vertical performances of the device under different excitation frequencies and amplitudes has been carried out in this paper. The characteristics of the vertical properties including the initial stiffness, the energy dissipation stiffness, the energy dissipation per cycle and the vertical damping ratio changing with excitation frequency and amplitude were studied, and the formulas describing the characteristics were proposed. It can be concluded that the initial stiffness and the energy dissipation stiffness increase slightly with increasing frequency, while the energy dissipation per cycle and the damping ratio decrease slightly with increasing frequency, the initial stiffness, the energy dissipation stiffness and the damping ratio will decrease slightly with increasing excitation amplitudes, and the proposed formulas can describe the vertical properties of the multi-dimensional earthquake isolation and mitigation device changing with excitation frequency and amplitude