Design, synthesis and antifungal activity of novel 1,4-benzoxazin-3-one derivatives containing an acylhydrazone moiety

A series of 1,4-benzoxazin-3-one derivatives containing an acylhydrazone moiety were designed, synthesized and evaluated for their in vitro antifungal activities against Gibberella zeae, Pellicularia sasakii, Phytophthora infestans, Capsicum wilt, and Phytophthora capsica. The structures of target compounds were characterized by 1H NMR, 13H NMR, 19F NMR and HRMS. The preliminary antifungal evaluation of all target compounds showed that some target compounds possessed moderate to good activities against G. zeae, P. sasakii, P. infestans and C. wilt. Among them, compounds 5L and 5o exhibited noticeable inhibition effects against G. zeae with the EC50 values (effective concentration for 50% activity) of 20.06 and 23.17 μg/ml, respectively, which were even nearly double effective than that of hymexazol (40.51 μg/ml). Meanwhile, compound 5q displayed a notable inhibitory effect toward P. sasakii, with the EC50 value of 26.66 μg/ml, which was better than that of hymexazol (32.77 μg/ml). In addition, compound 5r yielded the EC50 value of 15.37 μg/ml against P. infestans, which was less than those of hymexazol (18.35 μg/ml) and carbendazim (34.41 μg/ml). Eventually, compound 5p showed higher inhibitory effect against C. wilt, with EC50 value of 26.76 μg/ml, which was better than that of hymexazol (>50 μg/ml)

In Situ Carbon Coated LiNi 0.5

Carbon coated spinel LiNi0.5Mn1.5O4 were prepared by spray-drying using prepolymer of melamine formaldehyde resin (PMF) as carbon source of carbon coating layer. The PMF carbon coated LiNi0.5Mn1.5O4 was characterized by XRD, SEM, and other electrochemical measurements. The as-prepared lithium nickel manganese oxide has the cubic face-centered spinel structure with a space group of Fd3m. It showed good electrochemical performance as a cathode material for lithium ion battery. After 100 discharge and charge cycles at 0.5 C rate, the specific discharge capacity of carbon coated LiNi0.5Mn1.5O4 was 130 mAh·g−1, and the corresponding capacity retention was 98.8%. The 100th cycle specific discharge capacity at 10 C rate of carbon coated LiNi0.5Mn1.5O4 was 105.4 mAh·g−1, and even the corresponding capacity retention was 95.2%

Establishment of a Gene Detection System for Hotspot Mutations of Hearing Loss

Hearing loss is an etiologically heterogeneous trait with a high incidence in China. Though conventional newborn hearing screening program has been widely adopted, gene detection can significantly improve the means of early discovering genetic risk factors. Thus, simple and efficient methods with higher sensitivity and lower cost for detecting hotspot mutations of hearing loss are urgently requested. Here we established a mutation detection system based on multiple fluorescent probe technique, which can detect and genotype nine hotspot mutations of four prominent hearing loss-related genes in two reactions on a four-channel real-time PCR instrument, including GJB2 (rs750188782, rs80338943, rs1110333204, and rs80338939), GJB3 (rs74315319), SLC26A4 (rs111033313 and rs121908362), and mtDNA 12S rRNA (rs267606617 and rs267606619). This system is with high sensitivity that enables detecting as low as 10 DNA copies samples per reaction. A comparison study in 268 clinical samples showed that the detection system had 100% concordance to Sanger sequencing. Besides, blood and saliva samples can be directly detected without DNA extraction process, which greatly simplifies the manipulation. The new system with high sensitivity, accuracy, and specimen type compatibility can be expectedly a reliable tool in clinical application

Adaptive changes of gastro-fntero-pancreatic system endocrine cells in the black-spotted pond frog Rana nigromaculata after fasting

Changes in distribution density, morphology and secretory content of endocrine cells in the gastro-entero-pancreatic system of black-spotted frogs Rana nigromaculata before and after fasting were investigated using immunohistochemistry and antisera to six gut hormones. Six types of endocrine cells were detected in the digestive tract of Rana nigromaculata, including 5-hydroxytryptamine (5-HT), gastrin (GAS), somatostatin (SOM), glucagon (GLU), pancreatic polypeptide (PP) and vasoactive intestinal polypeptide (VIP) cells. After fasting, the density of 5-HT cells in the esophagus, cardia and fundus, GAS cells in fundus and pylorus, PP cells in the fundus decreased significantly(P 0.05), while SOM, GAS, GLU and PP cells increased significantly (P <0.01). Furthermore, the secretory content of GLU cells increased significantly. Considering their functionalities, our results indicate that the changes of GEP endocrine cells in Rana nigromaculata responded adaptively to starvation-induced stress [Current Zoology 55 (4):301–308, 2009]

High-Quality Epitaxial N Doped Graphene on SiC with Tunable Interfacial Interactions via Electron/Ion Bridges for Stable Lithium-Ion Storage

Highlights The intimate NG@SiC heterostructure has been constructed via a direct thermal decomposition method. The NG@SiC heterostructure anode delivers enhanced capacity and cycling stability both in the half-cell and in the full cell. DFT analysis reveals that this NG@SiC anode possesses lower lithium-ion adsorption energy and higher charge and discharge rates

Enhanced electrochemical performance of Li-rich layered oxide, Li1.2Mn0.54Co0.13Ni0.13O2, by surface modification derived from a MOF-assisted treatment

We developed a promising cathode material prepared by effective surface modification of Li-rich layered oxide (LLO) materials, using a Zr-based MOF (UIO-66-F4), as a precursor to produce in-situ MOF-derived ZrO2 (MDZ) coatings. A new method for F doping on LLO was also proposed. The MOF-assisted treatment renders a uniform nanoscale coating of ZrO2 and a porous structure of Li1.2Mn0.54Co0.13Ni0.13O2 hierarchical secondary microsphere. The rate capability, cycling stability, and first coulombic efficiency of LLO were significantly improved by the MOF-assisted treatment. The discharge capacity of the MOF-derived ZrO2 coated LLO (MDZ@LLO) material was 279 and 110.0 mAh g−1 at 0.1 C and 5 C, respectively. The capacity retention increased from 71.1% to 83.8% after 200 cycles at 1 C while the coulombic efficiency increased from 62% to 72% during the first cycle. Keywords: Lithium-ion battery, Lithium-rich layered oxide material, Surface modification, Fluorine dopin

Flower-like Heterogeneous Phosphorus-Doped Co₃S₄@Ni₃S₄ Nanoparticles as a Binder-free Electrode for Asymmetric All-Solid-State Supercapacitors

Through the hydrothermal method and the gas-phase phosphating method, the flower-like heterogeneous phosphorus-doped Co3S4@Ni3S4 was synthesized in situ on a nickel foam substrate as the binder-free electrode material for supercapacitors. Phosphorus-doped Co3S4@Ni3S4 electrode material combines the merits of transition metal sulfides and 3D porous network heterostructure, showing the excellent theoretical specific capacitance and the high specific surface area. The introduction of phosphorus atoms with an atomic radius larger than sulfur atoms can optimize the internal electronic structure and cause structural distortion. Therefore, the specific capacitance/specific capacity of this electrode can reach 3614 F g–1 (451 mAh g–1) at 1 A g–1 and still maintain the initial specific capacitance of 73% after 3000 cycles. The assembled P–Co3S4@Ni3S4-175//AC ASC device exhibits an ultra-high energy density of 72 Wh kg–1 at a power density of 800 W kg–1. Meanwhile, it can show extraordinary cyclic stability, with a retention rate of 91% after 5000 cycles. This work provides a feasible synthesis method to prepare the composite electrode materials for supercapacitors

The application of nanotechnology in kidney transplantation - supplementary material

Table S1 Nanotechnology for kidney transplantation</p

Effects of aeration rate on maturity and gaseous emissions during sewage sludge composting

This study investigated effects of aeration rate (AR) on maturity and gaseous emissions during sewage sludge composting, sewage sludge and corn stalks as the bulking agent were co-composted at different ARs (0.1, 0.2, 0.3 L·kg−1 dry matter (DM)·min−1). The thermophilic phase for the low and moderate AR treatments was able meet sanitation requirements, but too short to meet sanitation requirements in the high AR treatment. The high AR treatment was significantly different from the other treatments, and had the lowest electrical conductivity and highest E4/E6(absorbance ratio of wavelength 465 and 665 nm). The AR influences the nitrogen variations; high AR compost had the highest NH4+-N content and lowest NOx−-N content. The AR was the main factor influencing compost stability, but the AR had little impact on pH and the germination index. The moderate AR treatment had the highest NH3 emissions during composting, while the low AR treatment had the highest CH4 and N2O emissions. Based on our comprehensive investigation, the recommended AR for sludge composting is 0.2 L·kg−1 DM·min−1

DataSheet1_Design, synthesis and antifungal activity of novel 1,4-benzoxazin-3-one derivatives containing an acylhydrazone moiety.doc

A series of 1,4-benzoxazin-3-one derivatives containing an acylhydrazone moiety were designed, synthesized and evaluated for their in vitro antifungal activities against Gibberella zeae, Pellicularia sasakii, Phytophthora infestans, Capsicum wilt, and Phytophthora capsica. The structures of target compounds were characterized by 1H NMR, 13H NMR, 19F NMR and HRMS. The preliminary antifungal evaluation of all target compounds showed that some target compounds possessed moderate to good activities against G. zeae, P. sasakii, P. infestans and C. wilt. Among them, compounds 5L and 5o exhibited noticeable inhibition effects against G. zeae with the EC50 values (effective concentration for 50% activity) of 20.06 and 23.17 μg/ml, respectively, which were even nearly double effective than that of hymexazol (40.51 μg/ml). Meanwhile, compound 5q displayed a notable inhibitory effect toward P. sasakii, with the EC50 value of 26.66 μg/ml, which was better than that of hymexazol (32.77 μg/ml). In addition, compound 5r yielded the EC50 value of 15.37 μg/ml against P. infestans, which was less than those of hymexazol (18.35 μg/ml) and carbendazim (34.41 μg/ml). Eventually, compound 5p showed higher inhibitory effect against C. wilt, with EC50 value of 26.76 μg/ml, which was better than that of hymexazol (>50 μg/ml).</p