The earthquake sequence, surface rupture characteristics and engineering effects of the MS6.9 Qinghai Menyuan earthquake on January 8, 2022

At 01:45 on January 8, 2022, an MS6.9 earthquake occurred in Menyuan County, Haibei Tibetan Autonomous Prefecture, Qinghai Province. In order to gain a deeper understanding of the activity characteristics of this earthquake, the author conducted a preliminary investigation and analysis of the surface rupture zone and traffic system damage in the Liuhuanggou based on the investigation of earthquake disaster losses, combined with various methods such as drone low altitude photogrammetry and aftershock data analysis. The fault activity was mainly characterized by left-lateral strike-slip motion, accompanied by a certain thrust component, with a maximum left lateral displacement of 3.1 m. At the same time, the earthquake caused serious damage to the Liuhuanggou bridge and the Daliang tunnel of the Lanxin high-speed railway, mainly manifested as beam overturning and sliding, track distortion and deformation, and internal structure displacement and deformation of the tunnel. Finally, this article provides opinions and suggestions on the seismic fortification measures for bridges in the region

Single cell atlas for 11 non-model mammals, reptiles and birds.

The availability of viral entry factors is a prerequisite for the cross-species transmission of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Large-scale single-cell screening of animal cells could reveal the expression patterns of viral entry genes in different hosts. However, such exploration for SARS-CoV-2 remains limited. Here, we perform single-nucleus RNA sequencing for 11 non-model species, including pets (cat, dog, hamster, and lizard), livestock (goat and rabbit), poultry (duck and pigeon), and wildlife (pangolin, tiger, and deer), and investigated the co-expression of ACE2 and TMPRSS2. Furthermore, cross-species analysis of the lung cell atlas of the studied mammals, reptiles, and birds reveals core developmental programs, critical connectomes, and conserved regulatory circuits among these evolutionarily distant species. Overall, our work provides a compendium of gene expression profiles for non-model animals, which could be employed to identify potential SARS-CoV-2 target cells and putative zoonotic reservoirs

Effect of Zinc Aluminum Magnesium Coating on Spot-Welding Joint Properties of HC340LAD + ZM Steel

Zn-Al-Mg (zinc, aluminum and magnesium)-coated steel is gradually replacing traditional hot-dip galvanized steel due to its excellent corrosion resistance, self-healing properties and good surface hardness. However, the effect of Zn-Al-Mg coating on the resistance spot-welding joint properties of HC340LAD + ZM steel plates is not clear, and there are few systematic studies on it. In this paper, L16 (43) orthogonal experiments were designed on Zn-Al-Mg-coated steel HC340LAD + ZM (thickness = 1 mm). In addition, taking the tensile shear force as the main evaluation standard, the optimal spot-welding process properties could be achieved when the welding current, the welding time and the electrode loading were 10 kA, 14 cycles and 2.6 kN, respectively. On this basis, the formation mechanism, microstructure and corrosion properties of two plates of steels, with or without zinc, aluminum and magnesium coating under different welding times, were studied. The presence of Zn-Al-Mg coating slightly affected the mechanical properties of welding joints. However, the corrosion current of the body material containing Zn-Al-Mg plating was 7.17 times that of the uncoated plate

Effect of Zinc Aluminum Magnesium Coating on Spot-Welding Joint Properties of HC340LAD + ZM Steel

Zn-Al-Mg (zinc, aluminum and magnesium)-coated steel is gradually replacing traditional hot-dip galvanized steel due to its excellent corrosion resistance, self-healing properties and good surface hardness. However, the effect of Zn-Al-Mg coating on the resistance spot-welding joint properties of HC340LAD + ZM steel plates is not clear, and there are few systematic studies on it. In this paper, L16 (43) orthogonal experiments were designed on Zn-Al-Mg-coated steel HC340LAD + ZM (thickness = 1 mm). In addition, taking the tensile shear force as the main evaluation standard, the optimal spot-welding process properties could be achieved when the welding current, the welding time and the electrode loading were 10 kA, 14 cycles and 2.6 kN, respectively. On this basis, the formation mechanism, microstructure and corrosion properties of two plates of steels, with or without zinc, aluminum and magnesium coating under different welding times, were studied. The presence of Zn-Al-Mg coating slightly affected the mechanical properties of welding joints. However, the corrosion current of the body material containing Zn-Al-Mg plating was 7.17 times that of the uncoated plate

Screen Anti-influenza Lead Compounds That Target the PA_C Subunit of H5N1 Viral RNA Polymerase

<div><p>The avian influenza (H5N1) viral RNA polymerase protein PA_C was used as a target to screen nine chlorogenic acid derivatives for their polymerase inhibitor activity. Among them, seven compounds were PA_C ligands, and four inhibited influenza RNA polymerase activity. These results aid in the design of anti-influenza agents based on caffeoylquinic acid.</p> </div

Binder screening by relaxation-edited NMR.

<p>Spectra of 1,5-dicaffeoylquinic acid (compound b) in the absence (plots <b>a</b>, <b>b</b>) and presence (plots <b>c</b>, <b>d</b>) of PA_C, with the CPMG spin-lock time labeled beside each spectrum. The concentration of the small molecule and PA_C was 1.0×10⁻³ mol/L and 4.4×10⁻⁶ mol/L, respectively. The water peak located at δ 4.8 and 1 mM of TSP was added to the sample as a reference (δ 0). Signals of the small molecule in the absence of PA_C attenuated slightly when 600 ms of CPMG spin-lock was applied (plot <b>a</b>). These signals were eliminated at the same spin-lock time in the presence of PA_C (plot <b>c</b>). This difference should be ascribed to the increase in transverse relaxation rate (R₂) of the small molecule upon its binding to PA_C. The ligand peaks that attenuated when applying CPMG spin-lock in the presence of PA_C were marked with “*” in plots <b>b</b> and <b>d</b>.</p

Binding affinities of candidate compounds toward PA_C evaluated by visual docking, NMR, and SPR.

[a]<p>Candidates <b>f</b> and <b>g</b> were identified to be PA_C ligands, but the binding affinities were too weak to be evaluated by NMR methods.</p

Results of ApG primer extension assay.

<p>(<b>a</b>) The effects of candidate compounds <b>a</b>, <b>b</b>, <b>c</b>, <b>d</b>, <b>e</b>, <b>f</b>, and <b>g</b> on polymerase activity in an ApG primer extension assay. The concentrations of the small molecules were 5 mM. NP stands for the negative reference with “no polymerase”, and ND for the positive reference with “no drug.” (<b>b</b>) The quantification of the results from (<b>a</b>) obtained by phosphorimaging analysis. The results are the average of two independent experiments and the derivations are shown.</p