17,781 research outputs found
Beyond cultural and national identities : current re-evaluation of the Kominka literature from Taiwan\u27s Japanese period
This paper is an offshoot of a larger, ongoing project that intends to deal with the relationship between various artistic formations and the dominant culture in Taiwan\u27s post-1949 era. Though the lifting of martial law in 1987 has demarcated this era into two drastically different periods and a clearer contour of the new period seems to be just beginning to emerge in the mid-1990s, various cultural forces are still busily negotiating with each other. Nonetheless, there seems to be a general consensus as to what constitutes a core of the new dominant culture: the spirit of pen-t\u27u, or a nativist imperative that obliges one to treat Taiwan as the center in one\u27s cultural mapping. The primary driving force for this recent reconstitution of Taiwan\u27s dominant culture undoubtedly came from the momentous changes in the political arena in the post-martial law period. This rather crude factor, however, should not obscure our vision of the longer, more far-reaching evolutionary process of cultural change in contemporary Taiwan. Simply put, since the early 1980s, the older cultural hegemony has been seriously contested by forces coming from the Taiwanese cultural nationalism advocated in a vibrant pen-t\u27u (nativization) trend on the one hand, and from various radical cultural formations on the other. Limited by space, this paper will only deal with specific aspects of the nativization trend, with the main paradigm taken from the literary field. The paper will begin with a brief overview of the indigenous literary discourse in Taiwan’s post-1949 era, followed by analyses of recent scholarly re- evaluations of the Kominka literature from Taiwan’s Japanese period. Through this investigation, I hope to reach a better understanding of some important issues pertaining to contemporary cultural transformation in Taiwan, such as the role of cultural nationalism, the problem of identity construction, and efforts toward institutionalizing Taiwanese literary studies as an academic discipline
The asymptotic global power comparisons of the GMM overidentifying restrictions tests
In this paper, the asymptotic power comparisons of two versions of GMM overidentifying restrictions tests are conducted globally through the concept of approximate slopes. It is found that the GMM overidentifying restrictions test with the consistent mean deviation variance-covariance matrix estimator is more powerful than the test with the conventional non-mean deviation one. The results shed new light on the findings of Chang (2005) and Hall (2000).Approximate Slopes Overidentifying Restrictions Test.
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Effective genome editing and identification of a regiospecific gallic acid 4-O-glycosyltransferase in pomegranate (Punica granatum L.).
Pomegranate (Punica granatum L.) trees are woody perennials that bear colorful and nutritious fruits rich in phenolic metabolites, e.g., hydrolyzable tannins (HTs) and flavonoids. We here report genome editing and gene discovery in pomegranate hairy roots using Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/CRISPR-associated protein 9 (Cas9) (CRISPR/Cas9), coupled with transcriptome and biochemical analyses. Single guide RNAs (sgRNAs) were designed to target two UDP-dependent glycosyltransferases (UGTs), PgUGT84A23 and PgUGT84A24, which possess overlapping activities in β-glucogallin (a galloylglucose ester; biosynthetic precursor of HTs) biosynthesis. A unique accumulation of gallic acid 3-O- and 4-O-glucosides (galloylglucose ethers) was observed in the PgUGT84A23 and PgUGT84A24 dual CRISPR/Cas9-edited lines (i.e., ugt84a23 ugt84a24) but not the control (empty vector) or PgUGT84A23/PgUGT84A24 single edited lines (ugt84a23 or ugt84a24). Transcriptome and real-time qPCR analyses identified 11 UGTs with increased expression in the ugt84a23 ugt84a24 hairy roots compared to the controls. Of the 11 candidate UGTs, only PgUGT72BD1 used gallic acid as substrate and produced a regiospecific product gallic acid 4-O-glucoside. This work demonstrates that the CRISPR/Cas9 method can facilitate functional genomics studies in pomegranate and shows promise for capitalizing on the metabolic potential of pomegranate for germplasm improvement
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