Additional file 1 of Hemodynamics combined with inflammatory indicators exploring relationships between ischemic stroke and symptomatic middle cerebral artery atherosclerotic stenosis

Additional file 1: Supplementary Materials

Direct Synthesis and Practical Bandgap Estimation of Multilayer Arsenene Nanoribbons

Direct Synthesis and Practical Bandgap Estimation of Multilayer Arsenene Nanoribbon

PGCLCs derived from hc-iPSCs.

<p>Immunofluorescent staining of (A) BLIMP1 (green color) and (B) OCT4 (green color) in 4-day-old EBs cultured with PGC induction medium on hc-iPSC-1, -2, -5 and -6 (third to fifth column), PBMC derived iPSC0102 and iPSC0207 (right two column). H9 hESCs (left column) adapted in 4i medium was also detected for BLIMP1 and OCT4 antibodies. Noted that there was no BLIMP1 signals detected in undifferentiated H9 in 4i condition. All samples were doubly stained with SOX17 antibody (red color) sequentially. DAPI was used for counterstain (blue). Scale bar = 50μm. (C) Quantification of immunofluoresent staining of PGCLCs. BLIMP1/SOX17 and OCT4/SOX17 double positive cells were counted for PGCLCs and normalized with the number of DAPI. Percentage of PGCLCs per EB of all lines was presented and significance was compared with control hESC H9. ** indicates P<0.005. *** indicates P<0.001.</p

Summary of patient information.

<p>Summary of patient information.</p

<i>In vitro</i> differentiation and teratoma assay.

<p>(A) The germlayer specific markers SOX17 (endoderm, red color), TUJ1 (ectoderm, green color), and the BRYCHURY (mesoderm, red color) were detected after <i>in vitro</i> differentiation in hc-iPSCs. DNA was stained by DAPI (blue). (B) Expression of germlayer specific genes (<i>SOX17</i> for endoderm, <i>PAX6</i> for ectoderm, and <i>HAND1</i> for mesoderm) were all upregulated in day 10 EBs compared with hc-iPSCs. (C) Ciliated epithelium (endoderm), neuronal-like (ectoderm) and cartilage (mesoderm) cells were present in hc-iPSC derived teratomas (stained with H&E). Arrows indicate corresponding cell types.</p

<i>In vitro</i> characterizations of hc-iPSCs.

<p>(A) The growth curve of hc-iPSCs (hc-iPSC-1, 2, 5, 6), and hESC H9 (3 replicates per line). (B) Population doubling time of hc-iPSC lines and hESC H9. (C) Alkaline phosphatase activity was detected in hc-iPSC-1, -2, -5 and -6 (Blue color, scale bar = 1 mm). Immunofluorescent with antibodies against OCT4, SOX2, SSEA4, TRA-1-60 and TRA-1-81 were also detected in hc-iPSC lines. Scale bar = 100μm. (D) Flow cytometry confirmed the percentage of SSEA4 and TRA-1-60 positive cells in hc-iPSC lines (red color), as the numbers on graphs indicated, respectively. Isotype controls were shown in white color. (E) Semiquantitative PCR for expression of pluripotent genes. Exogeneous <i>NANOG</i> and <i>POU5F1</i> (<i>Exo-NANOG</i>, <i>Exo-POU5F1</i>) genes were undetectable and endogeneous genes (<i>Endo-NANOG</i>, <i>Endo-POU5F1</i>) were expressed in hc-iPSC lines and hESC H9. cDNA of cumulus cells (CCs) at passage 3 was from mixture of patients. (+): template plasmids which containing <i>POU5F1</i> or <i>NANOG</i>. An unexpected band was amplified by endogeneous NANOG primer in <i>NANOG</i> plasmid with incorrect size of PCR product, which may due to sequence similarity. (-): negative control without template. <i>GAPDH</i> was used for internal control. (F) X chromosome inactivating transcripts <i>XIST</i> were detected in mixture of CCs at passage 0 (P0 CCs), and passage 3 (P3 CCs), but nearly undetectable in hc-iPSCs. (G) hc-iPSCs showed normal female karyotypes without chromosome translocation, deletion or replication.</p

Derivation of hc-iPSCs.

<p>(A) Human cumulus-oocyte-complex (COC). (B) COCs treated with hyaluronidase for 1 minute. (C) Denudated oocyte. (D) Completely dissociated CCs. (E) Morphology of attached human CCs under phase contrast microscope. (F) Cultured CCs of spindle-like shape after passage. (G) Primarily formed iPSC colony after 25 days of induction. (H) hc-iPSCs after manually pick-up. Scale bar: 100 μm.</p

Thermally Strained Band Gap Engineering of Transition-Metal Dichalcogenide Bilayers with Enhanced Light–Matter Interaction toward Excellent Photodetectors

Integration of strain engineering of two-dimensional (2D) materials in order to enhance device performance is still a challenge. Here, we successfully demonstrated the thermally strained band gap engineering of transition-metal dichalcogenide bilayers by different thermal expansion coefficients between 2D materials and patterned sapphire structures, where MoS₂ bilayers were chosen as the demonstrated materials. In particular, a blue shift in the band gap of the MoS₂ bilayers can be tunable, displaying an extraordinary capability to drive electrons toward the electrode under the smaller driven bias, and the results were confirmed by simulation. A model to explain the thermal strain in the MoS₂ bilayers during the synthesis was proposed, which enables us to precisely predict the band gap-shifted behaviors on patterned sapphire structures with different angles. Furthermore, photodetectors with enhancement of 286% and 897% based on the strained MoS₂ on cone- and pyramid-patterned sapphire substrates were demonstrated, respectively