Description of Campanella hanchuanensis p. nov. (Ciliophora, Peritrichida): general morphology and isozyme pattern

Living and stained specimens of Campanella hanchuanensis sp. nov., collected near Hanchuan Power Plant, Hubei Province, China, were studied under light microscopy. Infraciliature was stained using a protargol staining technique. There is no bridge between concentric silver lines. The aboral ciliary wreath consists of 6 lines of basal bodies, with the middle 4 lines compacted and one lateral line each side separated. The other characters are similar to or the same as that described in Campanella umbellaria. The esterase isozyme was analyzed by micro-isoelectrofocussing. Campanella hanchuanensis sp. nov. has three bands of esterase near the cathode.Article信州大学理学部附属諏訪臨湖実験所報告 9: 87-94(1995)departmental bulletin pape

How perceived personalities of earlier contributors influence the content generation on online knowledge-sharing platforms?

Recent years have witnessed the prevalence of online knowledge-sharing platforms, in which user management and content creation are the central issues to the governance. Interactions take place through users’ linguistics, which helps shape users’ perceived personalities in the eyes of others. To understand the impact of perceived personality on online discussions, a novel method that combines natural language processing method and unsupervised learning is developed to extract contributors’ perceived personalities based on the contents they generated on the platform, which is further validated by a lab experiment. An empirical analysis is then carried out to unpack the role of different personality dimensions on online discussions. Our results reveal that the first contributor’s perceived conscientiousness and openness exhibit a significant but contrary role on content generation. Our method and empirical analysis can provide insights into the governance of online knowledge-sharing platforms

Self-partitioning SlipChip for slip-induced droplet formation and human papillomavirus viral load quantification with digital LAMP

Human papillomavirus (HPV) is one of the most common sexually transmitted infections worldwide, and persistent HPV infection can cause warts and even cancer. Nucleic acid analysis of HPV viral DNA can be very informative for the diagnosis and monitoring of HPV. Digital nucleic acid analysis, such as digital PCR and digital isothermal amplification, can provide sensitive detection and precise quantification of target nucleic acids, and its utility has been demonstrated in many biological research and medical diagnostic applications. A variety of methods have been developed for the generation of a large number of individual reaction partitions, a key requirement for digital nucleic acid analysis. However, an easily assembled and operated device for robust droplet formation without preprocessing devices, auxiliary instrumentation or control systems is still highly desired. In this paper, we present a self-partitioning SlipChip (sp-SlipChip) microfluidic device for the slip-induced generation of droplets to perform digital loop-mediated isothermal amplification (LAMP) for the detection and quantification of HPV DNA. In contrast to traditional SlipChip methods, which require the precise alignment of microfeatures, this sp-SlipChip utilized a design of “chain-of-pearls” continuous microfluidic channel that is independent of the overlapping of microfeatures on different plates to establish the fluidic path for reagent loading. Initiated by a simple slipping step, the aqueous solution can robustly self-partition into individual droplets by capillary pressure-driven flow. This advantage makes the sp-SlipChip very appealing for the point-of-care quantitative analysis of viral load. As a proof of concept, we performed digital LAMP on an sp-SlipChip to quantify human papillomaviruses (HPVs) 16 and 18 and tested this method with fifteen anonymous clinical samples