ECAY transcripts in stallion sperm.

<p>Agarose gel images showing RT-PCR amplicons of 7 ECAY genes and transcripts in stallion sperm.</p

Distribution and expression of mapped RNA sequence tags in the horse genome.

<p><b>Mb</b>–megabase-pair; <b>AC</b>–average coverage; <b>Mt</b>–mitochondrial genome; map information for horse chromosomes was retrieved from Ensemb (<a href="http://www.ensembl.org/index" target="_blank">http://www.ensembl.org/index</a>. html); * includes known and novel protein coding, miRNA, rRNA, snRNA, snoRNA and Misc RNA genes.</p

Summary statistics for mapped RNA sequence tags

<p>: (<b>a</b>) Comparison of mapped tags (AC≥1) between the two sperm samples; (<b>b</b>) Proportions of tags with very high (AC≥100), high (10</p

Comparison of RNA-seq data with current equine gene models:

<p>(a) <i>PKM2</i> showing 9 <i>in silico</i> prediction sites, of which two are positioned 5′ upstream to exon 1; (b) <i>CRISP3</i> with 3 <i>in silico</i> prediction sites, all located 5′ upstream to exon 1; (c) <i>PRM1</i> and <i>TNP2</i> cluster (the protamine cluster) with 12 <i>in silico</i> prediction sites of which only two align with <i>PRM1</i> and <i>TNP2</i> exons. Black boxes with numbers –exons in current gene models; blue boxes –very highly expressed tags (AC≥100); red boxes–highly expressed tags (10</p

Structural and functional annotations for mRNAs and ESTs with the highest AC values by RNA-seq.

<p>Structural and functional annotations for mRNAs and ESTs with the highest AC values by RNA-seq.</p

Venn diagram of transcripts detected in stallion sperm and testes by microarray analysis (SNR ≥2).

<p>Venn diagram of transcripts detected in stallion sperm and testes by microarray analysis (SNR ≥2).</p